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Effect of Icariin on Engineered 3D-Printed Porous Scaffolds for Cartilage Repair

In recent times, cartilage defects have been the most common athletic injuries, often leading to dreadful consequences such as osteoarthritis, pain, joint deformities, and other symptoms. It is also evident that damage to articular cartilage is often difficult to recover or self-heal because of poor...

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Autores principales: Kankala, Ranjith Kumar, Lu, Feng-Jun, Liu, Chen-Guang, Zhang, Shan-Shan, Chen, Ai-Zheng, Wang, Shi-Bin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6119946/
https://www.ncbi.nlm.nih.gov/pubmed/30096899
http://dx.doi.org/10.3390/ma11081390
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author Kankala, Ranjith Kumar
Lu, Feng-Jun
Liu, Chen-Guang
Zhang, Shan-Shan
Chen, Ai-Zheng
Wang, Shi-Bin
author_facet Kankala, Ranjith Kumar
Lu, Feng-Jun
Liu, Chen-Guang
Zhang, Shan-Shan
Chen, Ai-Zheng
Wang, Shi-Bin
author_sort Kankala, Ranjith Kumar
collection PubMed
description In recent times, cartilage defects have been the most common athletic injuries, often leading to dreadful consequences such as osteoarthritis, pain, joint deformities, and other symptoms. It is also evident that damage to articular cartilage is often difficult to recover or self-heal because of poor vascular, nervous, and lymphatic supplies. Moreover, cartilage cells have poor regeneration ability and high maturity. Inspired by these facts and the rapid advances in the field of tissue engineering (TE), we fabricated highly porous three-dimensional (3D) scaffold architectures based on cell-responsive polymeric inks, i.e., sodium alginate and gelatin (SA-Gel, 1:3 ratio), by a novel 3D printing method. Moreover, the effect of various processing parameters was systematically investigated. The printed scaffolds of polymer composites gels with excellent transparency, moderate viscosity, and excellent fluid properties showed good surface morphology, better thermal stability and swelling effect, and unique interconnected porous architectures at the optimized operating parameters. In vitro cell proliferation experiments of these cytocompatible scaffolds showed the excellent adhesion rate and growth behavior of chondrocytes. In addition, the porous architectures facilitated the efficient distribution of cells with only a few remaining on the surface, which was confirmed by confocal laser scanning microscopic (CLSM) observations. Icariin (ICA) addition at a concentration of 10 μg/mL further significantly enhanced the proliferation of chondrocytes. We envision that these cell-responsive polymeric inks in the presence of growth regulators like ICA may have potential in engineering complex tissue constructs toward diverse applications in TE.
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spelling pubmed-61199462018-09-05 Effect of Icariin on Engineered 3D-Printed Porous Scaffolds for Cartilage Repair Kankala, Ranjith Kumar Lu, Feng-Jun Liu, Chen-Guang Zhang, Shan-Shan Chen, Ai-Zheng Wang, Shi-Bin Materials (Basel) Article In recent times, cartilage defects have been the most common athletic injuries, often leading to dreadful consequences such as osteoarthritis, pain, joint deformities, and other symptoms. It is also evident that damage to articular cartilage is often difficult to recover or self-heal because of poor vascular, nervous, and lymphatic supplies. Moreover, cartilage cells have poor regeneration ability and high maturity. Inspired by these facts and the rapid advances in the field of tissue engineering (TE), we fabricated highly porous three-dimensional (3D) scaffold architectures based on cell-responsive polymeric inks, i.e., sodium alginate and gelatin (SA-Gel, 1:3 ratio), by a novel 3D printing method. Moreover, the effect of various processing parameters was systematically investigated. The printed scaffolds of polymer composites gels with excellent transparency, moderate viscosity, and excellent fluid properties showed good surface morphology, better thermal stability and swelling effect, and unique interconnected porous architectures at the optimized operating parameters. In vitro cell proliferation experiments of these cytocompatible scaffolds showed the excellent adhesion rate and growth behavior of chondrocytes. In addition, the porous architectures facilitated the efficient distribution of cells with only a few remaining on the surface, which was confirmed by confocal laser scanning microscopic (CLSM) observations. Icariin (ICA) addition at a concentration of 10 μg/mL further significantly enhanced the proliferation of chondrocytes. We envision that these cell-responsive polymeric inks in the presence of growth regulators like ICA may have potential in engineering complex tissue constructs toward diverse applications in TE. MDPI 2018-08-09 /pmc/articles/PMC6119946/ /pubmed/30096899 http://dx.doi.org/10.3390/ma11081390 Text en © 2018 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Kankala, Ranjith Kumar
Lu, Feng-Jun
Liu, Chen-Guang
Zhang, Shan-Shan
Chen, Ai-Zheng
Wang, Shi-Bin
Effect of Icariin on Engineered 3D-Printed Porous Scaffolds for Cartilage Repair
title Effect of Icariin on Engineered 3D-Printed Porous Scaffolds for Cartilage Repair
title_full Effect of Icariin on Engineered 3D-Printed Porous Scaffolds for Cartilage Repair
title_fullStr Effect of Icariin on Engineered 3D-Printed Porous Scaffolds for Cartilage Repair
title_full_unstemmed Effect of Icariin on Engineered 3D-Printed Porous Scaffolds for Cartilage Repair
title_short Effect of Icariin on Engineered 3D-Printed Porous Scaffolds for Cartilage Repair
title_sort effect of icariin on engineered 3d-printed porous scaffolds for cartilage repair
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6119946/
https://www.ncbi.nlm.nih.gov/pubmed/30096899
http://dx.doi.org/10.3390/ma11081390
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