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New Insights into GFAP Negative Astrocytes in Calbindin D28k Immunoreactive Astrocytes
Glial fibrillary acidic protein (GFAP) is commonly used as a specific marker for the identification of astrocytes. Nevertheless, it is known from the literature that astrocytes in situ in contrast to cultured astrocytes may feature lower levels of GFAP. In order to characterize the properties of GFA...
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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MDPI
2018
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6119979/ https://www.ncbi.nlm.nih.gov/pubmed/30081456 http://dx.doi.org/10.3390/brainsci8080143 |
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author | Xu, Jie |
author_facet | Xu, Jie |
author_sort | Xu, Jie |
collection | PubMed |
description | Glial fibrillary acidic protein (GFAP) is commonly used as a specific marker for the identification of astrocytes. Nevertheless, it is known from the literature that astrocytes in situ in contrast to cultured astrocytes may feature lower levels of GFAP. In order to characterize the properties of GFAP in Calbindin D28k immunoreactive astrocytes, we use primary astrocyte cultures from cells of new-born mice. A double fluorescence immunocytochemical analysis reveals that GFAP in cultured Calbindin D28k astrocytes behaves differently depending on whether the medium contains foetal bovine serum (FBS) or not. The novelty in our study is, however, that a high percentage of Calbindin D28k cultured astrocytes in a medium with 10% FBS are GFAP negative. In addition, the study shows that Calbindin D28k astrocytes have (i) a different morphology and (ii) a higher concentration of Calbindin D28k in the nucleus than in the cytoplasm. The study provides new evidence that in order to fully understand the characteristics of astrocytes, astrocytes which are Calbindin D28k positive have to be investigated. |
format | Online Article Text |
id | pubmed-6119979 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-61199792018-09-06 New Insights into GFAP Negative Astrocytes in Calbindin D28k Immunoreactive Astrocytes Xu, Jie Brain Sci Article Glial fibrillary acidic protein (GFAP) is commonly used as a specific marker for the identification of astrocytes. Nevertheless, it is known from the literature that astrocytes in situ in contrast to cultured astrocytes may feature lower levels of GFAP. In order to characterize the properties of GFAP in Calbindin D28k immunoreactive astrocytes, we use primary astrocyte cultures from cells of new-born mice. A double fluorescence immunocytochemical analysis reveals that GFAP in cultured Calbindin D28k astrocytes behaves differently depending on whether the medium contains foetal bovine serum (FBS) or not. The novelty in our study is, however, that a high percentage of Calbindin D28k cultured astrocytes in a medium with 10% FBS are GFAP negative. In addition, the study shows that Calbindin D28k astrocytes have (i) a different morphology and (ii) a higher concentration of Calbindin D28k in the nucleus than in the cytoplasm. The study provides new evidence that in order to fully understand the characteristics of astrocytes, astrocytes which are Calbindin D28k positive have to be investigated. MDPI 2018-08-03 /pmc/articles/PMC6119979/ /pubmed/30081456 http://dx.doi.org/10.3390/brainsci8080143 Text en © 2018 by the author. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Xu, Jie New Insights into GFAP Negative Astrocytes in Calbindin D28k Immunoreactive Astrocytes |
title | New Insights into GFAP Negative Astrocytes in Calbindin D28k Immunoreactive Astrocytes |
title_full | New Insights into GFAP Negative Astrocytes in Calbindin D28k Immunoreactive Astrocytes |
title_fullStr | New Insights into GFAP Negative Astrocytes in Calbindin D28k Immunoreactive Astrocytes |
title_full_unstemmed | New Insights into GFAP Negative Astrocytes in Calbindin D28k Immunoreactive Astrocytes |
title_short | New Insights into GFAP Negative Astrocytes in Calbindin D28k Immunoreactive Astrocytes |
title_sort | new insights into gfap negative astrocytes in calbindin d28k immunoreactive astrocytes |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6119979/ https://www.ncbi.nlm.nih.gov/pubmed/30081456 http://dx.doi.org/10.3390/brainsci8080143 |
work_keys_str_mv | AT xujie newinsightsintogfapnegativeastrocytesincalbindind28kimmunoreactiveastrocytes |