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Decreasing miRNA sequencing bias using a single adapter and circularization approach

The ability to accurately quantify all the microRNAs (miRNAs) in a sample is important for understanding miRNA biology and for development of new biomarkers and therapeutic targets. We develop a new method for preparing miRNA sequencing libraries, RealSeq®-AC, that involves ligating the miRNAs with...

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Detalles Bibliográficos
Autores principales: Barberán-Soler, Sergio, Vo, Jenny M., Hogans, Ryan E., Dallas, Anne, Johnston, Brian H., Kazakov, Sergei A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6120088/
https://www.ncbi.nlm.nih.gov/pubmed/30173660
http://dx.doi.org/10.1186/s13059-018-1488-z