Noncoding RNA Transcripts during Differentiation of Induced Pluripotent Stem Cells into Hepatocytes

Recent advances in the stem cell field allow to obtain many human tissues in vitro. However, hepatic differentiation of induced pluripotent stem cells (iPSCs) still remains challenging. Hepatocyte-like cells (HLCs) obtained after differentiation resemble more fetal liver hepatocytes. MicroRNAs (miRN...

Descripción completa

Detalles Bibliográficos
Autores principales: Skrzypczyk, Aniela, Kehr, Stephanie, Krystel, Ilona, Bernhart, Stephan H., Giri, Shibashish, Bader, Augustinus, Stadler, Peter F.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi 2018
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6120260/
https://www.ncbi.nlm.nih.gov/pubmed/30210551
http://dx.doi.org/10.1155/2018/5692840
_version_ 1783352234802675712
author Skrzypczyk, Aniela
Kehr, Stephanie
Krystel, Ilona
Bernhart, Stephan H.
Giri, Shibashish
Bader, Augustinus
Stadler, Peter F.
author_facet Skrzypczyk, Aniela
Kehr, Stephanie
Krystel, Ilona
Bernhart, Stephan H.
Giri, Shibashish
Bader, Augustinus
Stadler, Peter F.
author_sort Skrzypczyk, Aniela
collection PubMed
description Recent advances in the stem cell field allow to obtain many human tissues in vitro. However, hepatic differentiation of induced pluripotent stem cells (iPSCs) still remains challenging. Hepatocyte-like cells (HLCs) obtained after differentiation resemble more fetal liver hepatocytes. MicroRNAs (miRNA) play an important role in the differentiation process. Here, we analysed noncoding RNA profiles from the last stages of differentiation and compare them to hepatocytes. Our results show that HLCs maintain an epithelial character and express miRNA which can block hepatocyte maturation by inhibiting the epithelial-mesenchymal transition (EMT). Additionally, we identified differentially expressed small nucleolar RNAs (snoRNAs) and discovered novel noncoding RNA (ncRNA) genes.
format Online
Article
Text
id pubmed-6120260
institution National Center for Biotechnology Information
language English
publishDate 2018
publisher Hindawi
record_format MEDLINE/PubMed
spelling pubmed-61202602018-09-12 Noncoding RNA Transcripts during Differentiation of Induced Pluripotent Stem Cells into Hepatocytes Skrzypczyk, Aniela Kehr, Stephanie Krystel, Ilona Bernhart, Stephan H. Giri, Shibashish Bader, Augustinus Stadler, Peter F. Stem Cells Int Research Article Recent advances in the stem cell field allow to obtain many human tissues in vitro. However, hepatic differentiation of induced pluripotent stem cells (iPSCs) still remains challenging. Hepatocyte-like cells (HLCs) obtained after differentiation resemble more fetal liver hepatocytes. MicroRNAs (miRNA) play an important role in the differentiation process. Here, we analysed noncoding RNA profiles from the last stages of differentiation and compare them to hepatocytes. Our results show that HLCs maintain an epithelial character and express miRNA which can block hepatocyte maturation by inhibiting the epithelial-mesenchymal transition (EMT). Additionally, we identified differentially expressed small nucleolar RNAs (snoRNAs) and discovered novel noncoding RNA (ncRNA) genes. Hindawi 2018-08-19 /pmc/articles/PMC6120260/ /pubmed/30210551 http://dx.doi.org/10.1155/2018/5692840 Text en Copyright © 2018 Aniela Skrzypczyk et al. http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. The publication of this article was funded by Max Planck.
spellingShingle Research Article
Skrzypczyk, Aniela
Kehr, Stephanie
Krystel, Ilona
Bernhart, Stephan H.
Giri, Shibashish
Bader, Augustinus
Stadler, Peter F.
Noncoding RNA Transcripts during Differentiation of Induced Pluripotent Stem Cells into Hepatocytes
title Noncoding RNA Transcripts during Differentiation of Induced Pluripotent Stem Cells into Hepatocytes
title_full Noncoding RNA Transcripts during Differentiation of Induced Pluripotent Stem Cells into Hepatocytes
title_fullStr Noncoding RNA Transcripts during Differentiation of Induced Pluripotent Stem Cells into Hepatocytes
title_full_unstemmed Noncoding RNA Transcripts during Differentiation of Induced Pluripotent Stem Cells into Hepatocytes
title_short Noncoding RNA Transcripts during Differentiation of Induced Pluripotent Stem Cells into Hepatocytes
title_sort noncoding rna transcripts during differentiation of induced pluripotent stem cells into hepatocytes
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6120260/
https://www.ncbi.nlm.nih.gov/pubmed/30210551
http://dx.doi.org/10.1155/2018/5692840
work_keys_str_mv AT skrzypczykaniela noncodingrnatranscriptsduringdifferentiationofinducedpluripotentstemcellsintohepatocytes
AT kehrstephanie noncodingrnatranscriptsduringdifferentiationofinducedpluripotentstemcellsintohepatocytes
AT krystelilona noncodingrnatranscriptsduringdifferentiationofinducedpluripotentstemcellsintohepatocytes
AT bernhartstephanh noncodingrnatranscriptsduringdifferentiationofinducedpluripotentstemcellsintohepatocytes
AT girishibashish noncodingrnatranscriptsduringdifferentiationofinducedpluripotentstemcellsintohepatocytes
AT baderaugustinus noncodingrnatranscriptsduringdifferentiationofinducedpluripotentstemcellsintohepatocytes
AT stadlerpeterf noncodingrnatranscriptsduringdifferentiationofinducedpluripotentstemcellsintohepatocytes

Ejemplares similares