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A Fully Automated Continuous‐Flow Platform for Fluorescence Quenching Studies and Stern–Volmer Analysis

Herein, we report the first fully automated continuous‐flow platform for fluorescence quenching studies and Stern–Volmer analysis. All the components of the platform were automated and controlled by a self‐written Python script. A user‐friendly software allows even inexperienced operators to perform...

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Detalles Bibliográficos
Autores principales: Kuijpers, Koen P. L., Bottecchia, Cecilia, Cambié, Dario, Drummen, Koen, König, Niels J., Noël, Timothy
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6120497/
https://www.ncbi.nlm.nih.gov/pubmed/29992682
http://dx.doi.org/10.1002/anie.201805632
Descripción
Sumario:Herein, we report the first fully automated continuous‐flow platform for fluorescence quenching studies and Stern–Volmer analysis. All the components of the platform were automated and controlled by a self‐written Python script. A user‐friendly software allows even inexperienced operators to perform automated screening of novel quenchers or Stern–Volmer analysis, thus accelerating and facilitating both reaction discovery and mechanistic studies. The operational simplicity of our system affords a time and labor reduction over batch methods while increasing the accuracy and reproducibility of the data produced. Finally, the applicability of our platform is elucidated through relevant case studies.