Cargando…
A Fully Automated Continuous‐Flow Platform for Fluorescence Quenching Studies and Stern–Volmer Analysis
Herein, we report the first fully automated continuous‐flow platform for fluorescence quenching studies and Stern–Volmer analysis. All the components of the platform were automated and controlled by a self‐written Python script. A user‐friendly software allows even inexperienced operators to perform...
Autores principales: | , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
John Wiley and Sons Inc.
2018
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6120497/ https://www.ncbi.nlm.nih.gov/pubmed/29992682 http://dx.doi.org/10.1002/anie.201805632 |
_version_ | 1783352282659684352 |
---|---|
author | Kuijpers, Koen P. L. Bottecchia, Cecilia Cambié, Dario Drummen, Koen König, Niels J. Noël, Timothy |
author_facet | Kuijpers, Koen P. L. Bottecchia, Cecilia Cambié, Dario Drummen, Koen König, Niels J. Noël, Timothy |
author_sort | Kuijpers, Koen P. L. |
collection | PubMed |
description | Herein, we report the first fully automated continuous‐flow platform for fluorescence quenching studies and Stern–Volmer analysis. All the components of the platform were automated and controlled by a self‐written Python script. A user‐friendly software allows even inexperienced operators to perform automated screening of novel quenchers or Stern–Volmer analysis, thus accelerating and facilitating both reaction discovery and mechanistic studies. The operational simplicity of our system affords a time and labor reduction over batch methods while increasing the accuracy and reproducibility of the data produced. Finally, the applicability of our platform is elucidated through relevant case studies. |
format | Online Article Text |
id | pubmed-6120497 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | John Wiley and Sons Inc. |
record_format | MEDLINE/PubMed |
spelling | pubmed-61204972018-09-05 A Fully Automated Continuous‐Flow Platform for Fluorescence Quenching Studies and Stern–Volmer Analysis Kuijpers, Koen P. L. Bottecchia, Cecilia Cambié, Dario Drummen, Koen König, Niels J. Noël, Timothy Angew Chem Int Ed Engl Communications Herein, we report the first fully automated continuous‐flow platform for fluorescence quenching studies and Stern–Volmer analysis. All the components of the platform were automated and controlled by a self‐written Python script. A user‐friendly software allows even inexperienced operators to perform automated screening of novel quenchers or Stern–Volmer analysis, thus accelerating and facilitating both reaction discovery and mechanistic studies. The operational simplicity of our system affords a time and labor reduction over batch methods while increasing the accuracy and reproducibility of the data produced. Finally, the applicability of our platform is elucidated through relevant case studies. John Wiley and Sons Inc. 2018-07-27 2018-08-27 /pmc/articles/PMC6120497/ /pubmed/29992682 http://dx.doi.org/10.1002/anie.201805632 Text en © 2018 The Authors. Published by Wiley-VCH Verlag GmbH & Co. KGaA. This is an open access article under the terms of the http://creativecommons.org/licenses/by-nc/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited and is not used for commercial purposes. |
spellingShingle | Communications Kuijpers, Koen P. L. Bottecchia, Cecilia Cambié, Dario Drummen, Koen König, Niels J. Noël, Timothy A Fully Automated Continuous‐Flow Platform for Fluorescence Quenching Studies and Stern–Volmer Analysis |
title | A Fully Automated Continuous‐Flow Platform for Fluorescence Quenching Studies and Stern–Volmer Analysis |
title_full | A Fully Automated Continuous‐Flow Platform for Fluorescence Quenching Studies and Stern–Volmer Analysis |
title_fullStr | A Fully Automated Continuous‐Flow Platform for Fluorescence Quenching Studies and Stern–Volmer Analysis |
title_full_unstemmed | A Fully Automated Continuous‐Flow Platform for Fluorescence Quenching Studies and Stern–Volmer Analysis |
title_short | A Fully Automated Continuous‐Flow Platform for Fluorescence Quenching Studies and Stern–Volmer Analysis |
title_sort | fully automated continuous‐flow platform for fluorescence quenching studies and stern–volmer analysis |
topic | Communications |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6120497/ https://www.ncbi.nlm.nih.gov/pubmed/29992682 http://dx.doi.org/10.1002/anie.201805632 |
work_keys_str_mv | AT kuijperskoenpl afullyautomatedcontinuousflowplatformforfluorescencequenchingstudiesandsternvolmeranalysis AT bottecchiacecilia afullyautomatedcontinuousflowplatformforfluorescencequenchingstudiesandsternvolmeranalysis AT cambiedario afullyautomatedcontinuousflowplatformforfluorescencequenchingstudiesandsternvolmeranalysis AT drummenkoen afullyautomatedcontinuousflowplatformforfluorescencequenchingstudiesandsternvolmeranalysis AT konignielsj afullyautomatedcontinuousflowplatformforfluorescencequenchingstudiesandsternvolmeranalysis AT noeltimothy afullyautomatedcontinuousflowplatformforfluorescencequenchingstudiesandsternvolmeranalysis AT kuijperskoenpl fullyautomatedcontinuousflowplatformforfluorescencequenchingstudiesandsternvolmeranalysis AT bottecchiacecilia fullyautomatedcontinuousflowplatformforfluorescencequenchingstudiesandsternvolmeranalysis AT cambiedario fullyautomatedcontinuousflowplatformforfluorescencequenchingstudiesandsternvolmeranalysis AT drummenkoen fullyautomatedcontinuousflowplatformforfluorescencequenchingstudiesandsternvolmeranalysis AT konignielsj fullyautomatedcontinuousflowplatformforfluorescencequenchingstudiesandsternvolmeranalysis AT noeltimothy fullyautomatedcontinuousflowplatformforfluorescencequenchingstudiesandsternvolmeranalysis |