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A Fully Automated Continuous‐Flow Platform for Fluorescence Quenching Studies and Stern–Volmer Analysis

Herein, we report the first fully automated continuous‐flow platform for fluorescence quenching studies and Stern–Volmer analysis. All the components of the platform were automated and controlled by a self‐written Python script. A user‐friendly software allows even inexperienced operators to perform...

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Detalles Bibliográficos
Autores principales: Kuijpers, Koen P. L., Bottecchia, Cecilia, Cambié, Dario, Drummen, Koen, König, Niels J., Noël, Timothy
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6120497/
https://www.ncbi.nlm.nih.gov/pubmed/29992682
http://dx.doi.org/10.1002/anie.201805632
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author Kuijpers, Koen P. L.
Bottecchia, Cecilia
Cambié, Dario
Drummen, Koen
König, Niels J.
Noël, Timothy
author_facet Kuijpers, Koen P. L.
Bottecchia, Cecilia
Cambié, Dario
Drummen, Koen
König, Niels J.
Noël, Timothy
author_sort Kuijpers, Koen P. L.
collection PubMed
description Herein, we report the first fully automated continuous‐flow platform for fluorescence quenching studies and Stern–Volmer analysis. All the components of the platform were automated and controlled by a self‐written Python script. A user‐friendly software allows even inexperienced operators to perform automated screening of novel quenchers or Stern–Volmer analysis, thus accelerating and facilitating both reaction discovery and mechanistic studies. The operational simplicity of our system affords a time and labor reduction over batch methods while increasing the accuracy and reproducibility of the data produced. Finally, the applicability of our platform is elucidated through relevant case studies.
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spelling pubmed-61204972018-09-05 A Fully Automated Continuous‐Flow Platform for Fluorescence Quenching Studies and Stern–Volmer Analysis Kuijpers, Koen P. L. Bottecchia, Cecilia Cambié, Dario Drummen, Koen König, Niels J. Noël, Timothy Angew Chem Int Ed Engl Communications Herein, we report the first fully automated continuous‐flow platform for fluorescence quenching studies and Stern–Volmer analysis. All the components of the platform were automated and controlled by a self‐written Python script. A user‐friendly software allows even inexperienced operators to perform automated screening of novel quenchers or Stern–Volmer analysis, thus accelerating and facilitating both reaction discovery and mechanistic studies. The operational simplicity of our system affords a time and labor reduction over batch methods while increasing the accuracy and reproducibility of the data produced. Finally, the applicability of our platform is elucidated through relevant case studies. John Wiley and Sons Inc. 2018-07-27 2018-08-27 /pmc/articles/PMC6120497/ /pubmed/29992682 http://dx.doi.org/10.1002/anie.201805632 Text en © 2018 The Authors. Published by Wiley-VCH Verlag GmbH & Co. KGaA. This is an open access article under the terms of the http://creativecommons.org/licenses/by-nc/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited and is not used for commercial purposes.
spellingShingle Communications
Kuijpers, Koen P. L.
Bottecchia, Cecilia
Cambié, Dario
Drummen, Koen
König, Niels J.
Noël, Timothy
A Fully Automated Continuous‐Flow Platform for Fluorescence Quenching Studies and Stern–Volmer Analysis
title A Fully Automated Continuous‐Flow Platform for Fluorescence Quenching Studies and Stern–Volmer Analysis
title_full A Fully Automated Continuous‐Flow Platform for Fluorescence Quenching Studies and Stern–Volmer Analysis
title_fullStr A Fully Automated Continuous‐Flow Platform for Fluorescence Quenching Studies and Stern–Volmer Analysis
title_full_unstemmed A Fully Automated Continuous‐Flow Platform for Fluorescence Quenching Studies and Stern–Volmer Analysis
title_short A Fully Automated Continuous‐Flow Platform for Fluorescence Quenching Studies and Stern–Volmer Analysis
title_sort fully automated continuous‐flow platform for fluorescence quenching studies and stern–volmer analysis
topic Communications
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6120497/
https://www.ncbi.nlm.nih.gov/pubmed/29992682
http://dx.doi.org/10.1002/anie.201805632
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