A novel DCL2-dependent miRNA pathway in tomato affects susceptibility to RNA viruses

Tomato Dicer-like2 (slDCL2) is a key component of resistance pathways against potato virus X (PVX) and tobacco mosaic virus (TMV). It is also required for production of endogenous small RNAs, including miR6026 and other noncanonical microRNAs (miRNAs). The slDCL2 mRNAs are targets of these slDCL2-de...

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Autores principales: Wang, Zhengming, Hardcastle, Thomas J., Canto Pastor, Alex, Yip, Wing Hin, Tang, Shuoya, Baulcombe, David C.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Cold Spring Harbor Laboratory Press 2018
Materias:
Research Communication
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6120711/
https://www.ncbi.nlm.nih.gov/pubmed/30150254
http://dx.doi.org/10.1101/gad.313601.118
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author Wang, Zhengming
Hardcastle, Thomas J.
Canto Pastor, Alex
Yip, Wing Hin
Tang, Shuoya
Baulcombe, David C.
author_facet Wang, Zhengming
Hardcastle, Thomas J.
Canto Pastor, Alex
Yip, Wing Hin
Tang, Shuoya
Baulcombe, David C.
author_sort Wang, Zhengming
collection PubMed
description Tomato Dicer-like2 (slDCL2) is a key component of resistance pathways against potato virus X (PVX) and tobacco mosaic virus (TMV). It is also required for production of endogenous small RNAs, including miR6026 and other noncanonical microRNAs (miRNAs). The slDCL2 mRNAs are targets of these slDCL2-dependent RNAs in a feedback loop that was disrupted by target mimic RNAs of miR6026. In lines expressing these RNAs, there was correspondingly enhanced resistance against PVX and TMV. These findings illustrate a novel miRNA pathway in plants and a crop protection strategy in which miRNA target mimicry elevates expression of defense-related mRNAs.
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spelling pubmed-61207112019-03-01 A novel DCL2-dependent miRNA pathway in tomato affects susceptibility to RNA viruses Wang, Zhengming Hardcastle, Thomas J. Canto Pastor, Alex Yip, Wing Hin Tang, Shuoya Baulcombe, David C. Genes Dev Research Communication Tomato Dicer-like2 (slDCL2) is a key component of resistance pathways against potato virus X (PVX) and tobacco mosaic virus (TMV). It is also required for production of endogenous small RNAs, including miR6026 and other noncanonical microRNAs (miRNAs). The slDCL2 mRNAs are targets of these slDCL2-dependent RNAs in a feedback loop that was disrupted by target mimic RNAs of miR6026. In lines expressing these RNAs, there was correspondingly enhanced resistance against PVX and TMV. These findings illustrate a novel miRNA pathway in plants and a crop protection strategy in which miRNA target mimicry elevates expression of defense-related mRNAs. Cold Spring Harbor Laboratory Press 2018-09-01 /pmc/articles/PMC6120711/ /pubmed/30150254 http://dx.doi.org/10.1101/gad.313601.118 Text en © 2018 Wang et al.; Published by Cold Spring Harbor Laboratory Press http://creativecommons.org/licenses/by-nc/4.0/ This article is distributed exclusively by Cold Spring Harbor Laboratory Press for the first six months after the full-issue publication date (see http://genesdev.cshlp.org/site/misc/terms.xhtml). After six months, it is available under a Creative Commons License (Attribution-NonCommercial 4.0 International), as described at http://creativecommons.org/licenses/by-nc/4.0/.
spellingShingle Research Communication
Wang, Zhengming
Hardcastle, Thomas J.
Canto Pastor, Alex
Yip, Wing Hin
Tang, Shuoya
Baulcombe, David C.
A novel DCL2-dependent miRNA pathway in tomato affects susceptibility to RNA viruses
title A novel DCL2-dependent miRNA pathway in tomato affects susceptibility to RNA viruses
title_full A novel DCL2-dependent miRNA pathway in tomato affects susceptibility to RNA viruses
title_fullStr A novel DCL2-dependent miRNA pathway in tomato affects susceptibility to RNA viruses
title_full_unstemmed A novel DCL2-dependent miRNA pathway in tomato affects susceptibility to RNA viruses
title_short A novel DCL2-dependent miRNA pathway in tomato affects susceptibility to RNA viruses
title_sort novel dcl2-dependent mirna pathway in tomato affects susceptibility to rna viruses
topic Research Communication
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6120711/
https://www.ncbi.nlm.nih.gov/pubmed/30150254
http://dx.doi.org/10.1101/gad.313601.118
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