Quantum Dot-Based Probes for Labeling and Imaging of Cells that Express Matrix Metalloproteinases

[Image: see text] The practical synthesis of novel multivalent fluorescent quantum-dot-based probes to target cellular matrix metalloproteinases (MMPs) (MT-MMPs) is reported. We show that these probes, which are decorated with a nanomolar water-soluble MMP inhibitor, can be used to label preferentia...

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Autores principales: Benito-Alifonso, David, Richichi, Barbara, Baldoneschi, Veronica, Berry, Monica, Fragai, Marco, Salerno, Gianluca, Galan, M. Carmen, Nativi, Cristina
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Chemical Society 2018
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6120729/
https://www.ncbi.nlm.nih.gov/pubmed/30198003
http://dx.doi.org/10.1021/acsomega.8b00633
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author Benito-Alifonso, David
Richichi, Barbara
Baldoneschi, Veronica
Berry, Monica
Fragai, Marco
Salerno, Gianluca
Galan, M. Carmen
Nativi, Cristina
author_facet Benito-Alifonso, David
Richichi, Barbara
Baldoneschi, Veronica
Berry, Monica
Fragai, Marco
Salerno, Gianluca
Galan, M. Carmen
Nativi, Cristina
author_sort Benito-Alifonso, David
collection PubMed
description [Image: see text] The practical synthesis of novel multivalent fluorescent quantum-dot-based probes to target cellular matrix metalloproteinases (MMPs) (MT-MMPs) is reported. We show that these probes, which are decorated with a nanomolar water-soluble MMP inhibitor, can be used to label preferentially the surface of cancer cells that are known to express MMPs while no binding was observed on cells that do not.
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spelling pubmed-61207292018-09-05 Quantum Dot-Based Probes for Labeling and Imaging of Cells that Express Matrix Metalloproteinases Benito-Alifonso, David Richichi, Barbara Baldoneschi, Veronica Berry, Monica Fragai, Marco Salerno, Gianluca Galan, M. Carmen Nativi, Cristina ACS Omega [Image: see text] The practical synthesis of novel multivalent fluorescent quantum-dot-based probes to target cellular matrix metalloproteinases (MMPs) (MT-MMPs) is reported. We show that these probes, which are decorated with a nanomolar water-soluble MMP inhibitor, can be used to label preferentially the surface of cancer cells that are known to express MMPs while no binding was observed on cells that do not. American Chemical Society 2018-08-23 /pmc/articles/PMC6120729/ /pubmed/30198003 http://dx.doi.org/10.1021/acsomega.8b00633 Text en Copyright © 2018 American Chemical Society This is an open access article published under a Creative Commons Attribution (CC-BY) License (http://pubs.acs.org/page/policy/authorchoice_ccby_termsofuse.html) , which permits unrestricted use, distribution and reproduction in any medium, provided the author and source are cited.
spellingShingle Benito-Alifonso, David
Richichi, Barbara
Baldoneschi, Veronica
Berry, Monica
Fragai, Marco
Salerno, Gianluca
Galan, M. Carmen
Nativi, Cristina
Quantum Dot-Based Probes for Labeling and Imaging of Cells that Express Matrix Metalloproteinases
title Quantum Dot-Based Probes for Labeling and Imaging of Cells that Express Matrix Metalloproteinases
title_full Quantum Dot-Based Probes for Labeling and Imaging of Cells that Express Matrix Metalloproteinases
title_fullStr Quantum Dot-Based Probes for Labeling and Imaging of Cells that Express Matrix Metalloproteinases
title_full_unstemmed Quantum Dot-Based Probes for Labeling and Imaging of Cells that Express Matrix Metalloproteinases
title_short Quantum Dot-Based Probes for Labeling and Imaging of Cells that Express Matrix Metalloproteinases
title_sort quantum dot-based probes for labeling and imaging of cells that express matrix metalloproteinases
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6120729/
https://www.ncbi.nlm.nih.gov/pubmed/30198003
http://dx.doi.org/10.1021/acsomega.8b00633
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