Unique Molecular Identifiers reveal a novel sequencing artefact with implications for RNA-Seq based gene expression analysis

Attaching Unique Molecular Identifiers (UMI) to RNA molecules in the first step of sequencing library preparation establishes a distinct identity for each input molecule. This makes it possible to eliminate the effects of PCR amplification bias, which is particularly important where many PCR cycles...

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Detalles Bibliográficos
Autores principales: Sena, Johnny A., Galotto, Giulia, Devitt, Nico P., Connick, Melanie C., Jacobi, Jennifer L., Umale, Pooja E., Vidali, Luis, Bell, Callum J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2018
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6120941/
https://www.ncbi.nlm.nih.gov/pubmed/30177820
http://dx.doi.org/10.1038/s41598-018-31064-7

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6120941/
https://www.ncbi.nlm.nih.gov/pubmed/30177820
http://dx.doi.org/10.1038/s41598-018-31064-7

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