Cargando…

Differential and Interactive Effects of Substrate Topography and Chemistry on Human Mesenchymal Stem Cell Gene Expression

Variations in substrate chemistry and the micro-structure were shown to have a significant effect on the biology of human mesenchymal stromal cells (hMSCs). This occurs when differences in the surface properties indirectly modulate pathways within numerous signaling networks that control cell fate....

Descripción completa

Detalles Bibliográficos
Autores principales: Li, Qiongfang, Zhang, Bo, Kasoju, Naresh, Ma, Jinmin, Yang, Aidong, Cui, Zhanfeng, Wang, Hui, Ye, Hua
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6121573/
https://www.ncbi.nlm.nih.gov/pubmed/30096912
http://dx.doi.org/10.3390/ijms19082344
_version_ 1783352499845988352
author Li, Qiongfang
Zhang, Bo
Kasoju, Naresh
Ma, Jinmin
Yang, Aidong
Cui, Zhanfeng
Wang, Hui
Ye, Hua
author_facet Li, Qiongfang
Zhang, Bo
Kasoju, Naresh
Ma, Jinmin
Yang, Aidong
Cui, Zhanfeng
Wang, Hui
Ye, Hua
author_sort Li, Qiongfang
collection PubMed
description Variations in substrate chemistry and the micro-structure were shown to have a significant effect on the biology of human mesenchymal stromal cells (hMSCs). This occurs when differences in the surface properties indirectly modulate pathways within numerous signaling networks that control cell fate. To understand how the surface features affect hMSC gene expression, we performed RNA-sequencing analysis of bone marrow-derived hMSCs cultured on tissue culture-treated polystyrene (TCP) and poly(l-lactide) (PLLA) based substrates of differing topography (Fl: flat and Fs: fibrous) and chemistry (Pr: pristine and Am: aminated). Whilst 80% of gene expression remained similar for cells cultured on test substrates, the analysis of differentially expressed genes (DEGs) revealed that surface topography significantly altered gene expression more than surface chemistry. The Fl and Fs topologies introduced opposite directional alternations in gene expression when compared to TCP control. In addition, the effect of chemical treatment interacted with that of topography in a synergistic manner with the Pr samples promoting more DEGs than Am samples in all gene ontology function groups. These findings not only highlight the significance of the culture surface on regulating the overall gene expression profile but also provide novel insights into cell-material interactions that could help further design the next-generation biomaterials to facilitate hMSC applications. At the same time, further studies are required to investigate whether or not the observations noted correlate with subsequent protein expression and functionality of cells.
format Online
Article
Text
id pubmed-6121573
institution National Center for Biotechnology Information
language English
publishDate 2018
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-61215732018-09-07 Differential and Interactive Effects of Substrate Topography and Chemistry on Human Mesenchymal Stem Cell Gene Expression Li, Qiongfang Zhang, Bo Kasoju, Naresh Ma, Jinmin Yang, Aidong Cui, Zhanfeng Wang, Hui Ye, Hua Int J Mol Sci Article Variations in substrate chemistry and the micro-structure were shown to have a significant effect on the biology of human mesenchymal stromal cells (hMSCs). This occurs when differences in the surface properties indirectly modulate pathways within numerous signaling networks that control cell fate. To understand how the surface features affect hMSC gene expression, we performed RNA-sequencing analysis of bone marrow-derived hMSCs cultured on tissue culture-treated polystyrene (TCP) and poly(l-lactide) (PLLA) based substrates of differing topography (Fl: flat and Fs: fibrous) and chemistry (Pr: pristine and Am: aminated). Whilst 80% of gene expression remained similar for cells cultured on test substrates, the analysis of differentially expressed genes (DEGs) revealed that surface topography significantly altered gene expression more than surface chemistry. The Fl and Fs topologies introduced opposite directional alternations in gene expression when compared to TCP control. In addition, the effect of chemical treatment interacted with that of topography in a synergistic manner with the Pr samples promoting more DEGs than Am samples in all gene ontology function groups. These findings not only highlight the significance of the culture surface on regulating the overall gene expression profile but also provide novel insights into cell-material interactions that could help further design the next-generation biomaterials to facilitate hMSC applications. At the same time, further studies are required to investigate whether or not the observations noted correlate with subsequent protein expression and functionality of cells. MDPI 2018-08-09 /pmc/articles/PMC6121573/ /pubmed/30096912 http://dx.doi.org/10.3390/ijms19082344 Text en © 2018 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Li, Qiongfang
Zhang, Bo
Kasoju, Naresh
Ma, Jinmin
Yang, Aidong
Cui, Zhanfeng
Wang, Hui
Ye, Hua
Differential and Interactive Effects of Substrate Topography and Chemistry on Human Mesenchymal Stem Cell Gene Expression
title Differential and Interactive Effects of Substrate Topography and Chemistry on Human Mesenchymal Stem Cell Gene Expression
title_full Differential and Interactive Effects of Substrate Topography and Chemistry on Human Mesenchymal Stem Cell Gene Expression
title_fullStr Differential and Interactive Effects of Substrate Topography and Chemistry on Human Mesenchymal Stem Cell Gene Expression
title_full_unstemmed Differential and Interactive Effects of Substrate Topography and Chemistry on Human Mesenchymal Stem Cell Gene Expression
title_short Differential and Interactive Effects of Substrate Topography and Chemistry on Human Mesenchymal Stem Cell Gene Expression
title_sort differential and interactive effects of substrate topography and chemistry on human mesenchymal stem cell gene expression
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6121573/
https://www.ncbi.nlm.nih.gov/pubmed/30096912
http://dx.doi.org/10.3390/ijms19082344
work_keys_str_mv AT liqiongfang differentialandinteractiveeffectsofsubstratetopographyandchemistryonhumanmesenchymalstemcellgeneexpression
AT zhangbo differentialandinteractiveeffectsofsubstratetopographyandchemistryonhumanmesenchymalstemcellgeneexpression
AT kasojunaresh differentialandinteractiveeffectsofsubstratetopographyandchemistryonhumanmesenchymalstemcellgeneexpression
AT majinmin differentialandinteractiveeffectsofsubstratetopographyandchemistryonhumanmesenchymalstemcellgeneexpression
AT yangaidong differentialandinteractiveeffectsofsubstratetopographyandchemistryonhumanmesenchymalstemcellgeneexpression
AT cuizhanfeng differentialandinteractiveeffectsofsubstratetopographyandchemistryonhumanmesenchymalstemcellgeneexpression
AT wanghui differentialandinteractiveeffectsofsubstratetopographyandchemistryonhumanmesenchymalstemcellgeneexpression
AT yehua differentialandinteractiveeffectsofsubstratetopographyandchemistryonhumanmesenchymalstemcellgeneexpression