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Occurrence and genotyping of Giardia duodenalis and Cryptosporidium in pre-weaned dairy calves in central Sichuan province, China

Giardia duodenalis and Cryptosporidium spp. are common human and animal pathogens. They have increasingly been reported in dairy calves in recent years; however, multilocus genotyping information for G. duodenalis and Cryptosporidium infecting pre-weaned dairy calves in southwestern China is limited...

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Autores principales: Zhong, Zhijun, Dan, Jiaming, Yan, Guangwen, Tu, Rui, Tian, Yinan, Cao, Suizhong, Shen, Liuhong, Deng, Junliang, Yu, Shumin, Geng, Yi, Gu, Xiaobin, Wang, Ya, Liu, Haifeng, Peng, Guangneng
Formato: Online Artículo Texto
Lenguaje:English
Publicado: EDP Sciences 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6121785/
https://www.ncbi.nlm.nih.gov/pubmed/30178744
http://dx.doi.org/10.1051/parasite/2018046
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author Zhong, Zhijun
Dan, Jiaming
Yan, Guangwen
Tu, Rui
Tian, Yinan
Cao, Suizhong
Shen, Liuhong
Deng, Junliang
Yu, Shumin
Geng, Yi
Gu, Xiaobin
Wang, Ya
Liu, Haifeng
Peng, Guangneng
author_facet Zhong, Zhijun
Dan, Jiaming
Yan, Guangwen
Tu, Rui
Tian, Yinan
Cao, Suizhong
Shen, Liuhong
Deng, Junliang
Yu, Shumin
Geng, Yi
Gu, Xiaobin
Wang, Ya
Liu, Haifeng
Peng, Guangneng
author_sort Zhong, Zhijun
collection PubMed
description Giardia duodenalis and Cryptosporidium spp. are common human and animal pathogens. They have increasingly been reported in dairy calves in recent years; however, multilocus genotyping information for G. duodenalis and Cryptosporidium infecting pre-weaned dairy calves in southwestern China is limited. In the present study, the prevalence of G. duodenalis and Cryptosporidium spp. in pre-weaned dairy calves in central Sichuan province was determined and the pathogens were analyzed molecularly. Of 278 fecal samples from pre-weaned dairy calves, 26 (9.4%) were positive for G. duodenalis and 40 (14.4%) were positive for Cryptosporidium spp. Cryptosporidium bovis (n = 28), Cryptosporidium ryanae (n = 5) and Cryptosporidium parvum (n = 7) were detected. All seven C. parvum isolates were successfully subtyped based on the gp60 gene sequence, and only IIdA15G1 was detected. Multilocus sequence typing of G. duodenalis based on beta-giardin (bg), triose phosphate isomerase (tpi) and glutamate dehydrogenase (gdh) genes revealed 19 different assemblage E multilocus genotypes (two known and 17 unpublished genotypes). Based on eBURST analysis, a high degree of genetic diversity within assemblage E was observed in pre-weaned dairy calves in Sichuan province. To the best of our knowledge, this is the first study using multilocus sequence typing and eBURST analysis to characterize G. duodenalis in pre-weaned dairy calves in southwestern China.
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spelling pubmed-61217852018-09-07 Occurrence and genotyping of Giardia duodenalis and Cryptosporidium in pre-weaned dairy calves in central Sichuan province, China Zhong, Zhijun Dan, Jiaming Yan, Guangwen Tu, Rui Tian, Yinan Cao, Suizhong Shen, Liuhong Deng, Junliang Yu, Shumin Geng, Yi Gu, Xiaobin Wang, Ya Liu, Haifeng Peng, Guangneng Parasite Research Article Giardia duodenalis and Cryptosporidium spp. are common human and animal pathogens. They have increasingly been reported in dairy calves in recent years; however, multilocus genotyping information for G. duodenalis and Cryptosporidium infecting pre-weaned dairy calves in southwestern China is limited. In the present study, the prevalence of G. duodenalis and Cryptosporidium spp. in pre-weaned dairy calves in central Sichuan province was determined and the pathogens were analyzed molecularly. Of 278 fecal samples from pre-weaned dairy calves, 26 (9.4%) were positive for G. duodenalis and 40 (14.4%) were positive for Cryptosporidium spp. Cryptosporidium bovis (n = 28), Cryptosporidium ryanae (n = 5) and Cryptosporidium parvum (n = 7) were detected. All seven C. parvum isolates were successfully subtyped based on the gp60 gene sequence, and only IIdA15G1 was detected. Multilocus sequence typing of G. duodenalis based on beta-giardin (bg), triose phosphate isomerase (tpi) and glutamate dehydrogenase (gdh) genes revealed 19 different assemblage E multilocus genotypes (two known and 17 unpublished genotypes). Based on eBURST analysis, a high degree of genetic diversity within assemblage E was observed in pre-weaned dairy calves in Sichuan province. To the best of our knowledge, this is the first study using multilocus sequence typing and eBURST analysis to characterize G. duodenalis in pre-weaned dairy calves in southwestern China. EDP Sciences 2018-09-04 /pmc/articles/PMC6121785/ /pubmed/30178744 http://dx.doi.org/10.1051/parasite/2018046 Text en © Z. Zhong et al., published by EDP Sciences, 2018 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Zhong, Zhijun
Dan, Jiaming
Yan, Guangwen
Tu, Rui
Tian, Yinan
Cao, Suizhong
Shen, Liuhong
Deng, Junliang
Yu, Shumin
Geng, Yi
Gu, Xiaobin
Wang, Ya
Liu, Haifeng
Peng, Guangneng
Occurrence and genotyping of Giardia duodenalis and Cryptosporidium in pre-weaned dairy calves in central Sichuan province, China
title Occurrence and genotyping of Giardia duodenalis and Cryptosporidium in pre-weaned dairy calves in central Sichuan province, China
title_full Occurrence and genotyping of Giardia duodenalis and Cryptosporidium in pre-weaned dairy calves in central Sichuan province, China
title_fullStr Occurrence and genotyping of Giardia duodenalis and Cryptosporidium in pre-weaned dairy calves in central Sichuan province, China
title_full_unstemmed Occurrence and genotyping of Giardia duodenalis and Cryptosporidium in pre-weaned dairy calves in central Sichuan province, China
title_short Occurrence and genotyping of Giardia duodenalis and Cryptosporidium in pre-weaned dairy calves in central Sichuan province, China
title_sort occurrence and genotyping of giardia duodenalis and cryptosporidium in pre-weaned dairy calves in central sichuan province, china
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6121785/
https://www.ncbi.nlm.nih.gov/pubmed/30178744
http://dx.doi.org/10.1051/parasite/2018046
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