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Effects of Different Pollens on Primary Metabolism and Lignin Biosynthesis in Pear

To investigate the effect of pollination on the fruit quality of ‘Dangshan Su’ pear, ‘Dangshan Su’ was fertilized by the pollen of ‘Wonhwang’ (Pyrus pyrifolia Nakai.) (DW) and ‘Jingbaili’ (Pyrus ussuriensis Maxim.) (DJ). The analysis of primary metabolites was achieved through untargeted metabolomic...

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Autores principales: Li, Shumei, Su, Xueqiang, Abdullah, Muhammad, Sun, Yanming, Li, Guohui, Cheng, Xi, Lin, Yi, Cai, Yongping, Jin, Qing
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6122022/
https://www.ncbi.nlm.nih.gov/pubmed/30072670
http://dx.doi.org/10.3390/ijms19082273
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author Li, Shumei
Su, Xueqiang
Abdullah, Muhammad
Sun, Yanming
Li, Guohui
Cheng, Xi
Lin, Yi
Cai, Yongping
Jin, Qing
author_facet Li, Shumei
Su, Xueqiang
Abdullah, Muhammad
Sun, Yanming
Li, Guohui
Cheng, Xi
Lin, Yi
Cai, Yongping
Jin, Qing
author_sort Li, Shumei
collection PubMed
description To investigate the effect of pollination on the fruit quality of ‘Dangshan Su’ pear, ‘Dangshan Su’ was fertilized by the pollen of ‘Wonhwang’ (Pyrus pyrifolia Nakai.) (DW) and ‘Jingbaili’ (Pyrus ussuriensis Maxim.) (DJ). The analysis of primary metabolites was achieved through untargeted metabolomics, and the quantitative analysis of intermediate metabolites of lignin synthesis was undertaken using targeted metabolomics. The untargeted metabolomics analysis was performed via gas chromatography-mass spectrometry (GC-MS). The targeted metabolomics analysis was performed using ultra-high-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) under the multiple reaction monitoring (MRM) mode. The results showed that the metabolite content was significantly different between DW and DJ. Compared with that in DJ, the sugar and amino acid content in DW was higher and the fatty acid content was lower at 47 days after pollination (DAPs), and the sugar, amino acid, and fatty acid content in DW was lower at 63 DAPs. The intermediate metabolites of lignin synthesis were analyzed using the orthogonal partial least squares discriminant analysis (OPLS-DA) model, and the differential metabolites at 47 DAPs were p-coumaric acid, ferulic acid, sinapaldehyde, coniferyl alcohol, and sinapyl alcohol. The differential significant metabolite at 63 DAPs was p-coumaric acid. At 47 DAPs and 63 DAPs, the p-coumaric acid level was significantly different, and the p-coumaric acid content was positively correlated with lignin synthesis. The pollination pollen affects the quality of ‘Dangshan Su’ pear fruit through regulation of the sugar, amino acid, and fatty acid content; at the same time, regulating the levels of intermediate metabolites of lignin synthesis, especially the p-coumaric acid content, to affect lignin synthesis ultimately affects the stone cell content and improves the quality of the pears.
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spelling pubmed-61220222018-09-07 Effects of Different Pollens on Primary Metabolism and Lignin Biosynthesis in Pear Li, Shumei Su, Xueqiang Abdullah, Muhammad Sun, Yanming Li, Guohui Cheng, Xi Lin, Yi Cai, Yongping Jin, Qing Int J Mol Sci Article To investigate the effect of pollination on the fruit quality of ‘Dangshan Su’ pear, ‘Dangshan Su’ was fertilized by the pollen of ‘Wonhwang’ (Pyrus pyrifolia Nakai.) (DW) and ‘Jingbaili’ (Pyrus ussuriensis Maxim.) (DJ). The analysis of primary metabolites was achieved through untargeted metabolomics, and the quantitative analysis of intermediate metabolites of lignin synthesis was undertaken using targeted metabolomics. The untargeted metabolomics analysis was performed via gas chromatography-mass spectrometry (GC-MS). The targeted metabolomics analysis was performed using ultra-high-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) under the multiple reaction monitoring (MRM) mode. The results showed that the metabolite content was significantly different between DW and DJ. Compared with that in DJ, the sugar and amino acid content in DW was higher and the fatty acid content was lower at 47 days after pollination (DAPs), and the sugar, amino acid, and fatty acid content in DW was lower at 63 DAPs. The intermediate metabolites of lignin synthesis were analyzed using the orthogonal partial least squares discriminant analysis (OPLS-DA) model, and the differential metabolites at 47 DAPs were p-coumaric acid, ferulic acid, sinapaldehyde, coniferyl alcohol, and sinapyl alcohol. The differential significant metabolite at 63 DAPs was p-coumaric acid. At 47 DAPs and 63 DAPs, the p-coumaric acid level was significantly different, and the p-coumaric acid content was positively correlated with lignin synthesis. The pollination pollen affects the quality of ‘Dangshan Su’ pear fruit through regulation of the sugar, amino acid, and fatty acid content; at the same time, regulating the levels of intermediate metabolites of lignin synthesis, especially the p-coumaric acid content, to affect lignin synthesis ultimately affects the stone cell content and improves the quality of the pears. MDPI 2018-08-02 /pmc/articles/PMC6122022/ /pubmed/30072670 http://dx.doi.org/10.3390/ijms19082273 Text en © 2018 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Li, Shumei
Su, Xueqiang
Abdullah, Muhammad
Sun, Yanming
Li, Guohui
Cheng, Xi
Lin, Yi
Cai, Yongping
Jin, Qing
Effects of Different Pollens on Primary Metabolism and Lignin Biosynthesis in Pear
title Effects of Different Pollens on Primary Metabolism and Lignin Biosynthesis in Pear
title_full Effects of Different Pollens on Primary Metabolism and Lignin Biosynthesis in Pear
title_fullStr Effects of Different Pollens on Primary Metabolism and Lignin Biosynthesis in Pear
title_full_unstemmed Effects of Different Pollens on Primary Metabolism and Lignin Biosynthesis in Pear
title_short Effects of Different Pollens on Primary Metabolism and Lignin Biosynthesis in Pear
title_sort effects of different pollens on primary metabolism and lignin biosynthesis in pear
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6122022/
https://www.ncbi.nlm.nih.gov/pubmed/30072670
http://dx.doi.org/10.3390/ijms19082273
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