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Prenylated Flavonoids from Roots of Glycyrrhiza uralensis Induce Differentiation of B16-F10 Melanoma Cells

Roots of Glycyrrhiza uralensis have been used as herbal medicine and natural sweetener. By activity-guided phytochemical investigation of the extracts from G. uralensis root, ten flavonoids, namely GF-1–GF-10, of which five were prenylated flavonoids, were found to show antiproliferative effects in...

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Detalles Bibliográficos
Autores principales: Zheng, Yunfeng, Wang, Huaiyou, Yang, Min, Peng, Guoping, Dong, Tina Ting Xia, Xu, Miranda Li, Tsim, Karl Wah Keung
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6122034/
https://www.ncbi.nlm.nih.gov/pubmed/30115883
http://dx.doi.org/10.3390/ijms19082422
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author Zheng, Yunfeng
Wang, Huaiyou
Yang, Min
Peng, Guoping
Dong, Tina Ting Xia
Xu, Miranda Li
Tsim, Karl Wah Keung
author_facet Zheng, Yunfeng
Wang, Huaiyou
Yang, Min
Peng, Guoping
Dong, Tina Ting Xia
Xu, Miranda Li
Tsim, Karl Wah Keung
author_sort Zheng, Yunfeng
collection PubMed
description Roots of Glycyrrhiza uralensis have been used as herbal medicine and natural sweetener. By activity-guided phytochemical investigation of the extracts from G. uralensis root, ten flavonoids, namely GF-1–GF-10, of which five were prenylated flavonoids, were found to show antiproliferative effects in melanoma B16-F10 cells. Three of the prenylated flavonoids, namely GF-1, GF-4 and GF-9, significantly induced the differentiation of B16-F10 cells; the inductions included increase of tyrosinase activity, tyrosinase protein, and melanin content. In GF-1 and GF-9 induced melanoma differentiation, the phosphorylation of p38 MAPK (mitogen activated potein kinase) was identified; while GF-4 could trigger the phosphorylation of PI3K/AKT (phosphatidylinositol 3-kinase/Protein Kinase B) signaling. However, application of GF-6 to the melanoma cells did not induce differentiation; but which promoted cell apoptotic signaling, i.e., increase levels of cleaved-PRAP, cleaved-caspase 3, and cleaved-caspase 9. These results suggested that different types of prenylated flavonoids from G. uralensis might have potential anticancer effects against melanoma cells by acting through different signaling pathways.
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spelling pubmed-61220342018-09-07 Prenylated Flavonoids from Roots of Glycyrrhiza uralensis Induce Differentiation of B16-F10 Melanoma Cells Zheng, Yunfeng Wang, Huaiyou Yang, Min Peng, Guoping Dong, Tina Ting Xia Xu, Miranda Li Tsim, Karl Wah Keung Int J Mol Sci Article Roots of Glycyrrhiza uralensis have been used as herbal medicine and natural sweetener. By activity-guided phytochemical investigation of the extracts from G. uralensis root, ten flavonoids, namely GF-1–GF-10, of which five were prenylated flavonoids, were found to show antiproliferative effects in melanoma B16-F10 cells. Three of the prenylated flavonoids, namely GF-1, GF-4 and GF-9, significantly induced the differentiation of B16-F10 cells; the inductions included increase of tyrosinase activity, tyrosinase protein, and melanin content. In GF-1 and GF-9 induced melanoma differentiation, the phosphorylation of p38 MAPK (mitogen activated potein kinase) was identified; while GF-4 could trigger the phosphorylation of PI3K/AKT (phosphatidylinositol 3-kinase/Protein Kinase B) signaling. However, application of GF-6 to the melanoma cells did not induce differentiation; but which promoted cell apoptotic signaling, i.e., increase levels of cleaved-PRAP, cleaved-caspase 3, and cleaved-caspase 9. These results suggested that different types of prenylated flavonoids from G. uralensis might have potential anticancer effects against melanoma cells by acting through different signaling pathways. MDPI 2018-08-16 /pmc/articles/PMC6122034/ /pubmed/30115883 http://dx.doi.org/10.3390/ijms19082422 Text en © 2018 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Zheng, Yunfeng
Wang, Huaiyou
Yang, Min
Peng, Guoping
Dong, Tina Ting Xia
Xu, Miranda Li
Tsim, Karl Wah Keung
Prenylated Flavonoids from Roots of Glycyrrhiza uralensis Induce Differentiation of B16-F10 Melanoma Cells
title Prenylated Flavonoids from Roots of Glycyrrhiza uralensis Induce Differentiation of B16-F10 Melanoma Cells
title_full Prenylated Flavonoids from Roots of Glycyrrhiza uralensis Induce Differentiation of B16-F10 Melanoma Cells
title_fullStr Prenylated Flavonoids from Roots of Glycyrrhiza uralensis Induce Differentiation of B16-F10 Melanoma Cells
title_full_unstemmed Prenylated Flavonoids from Roots of Glycyrrhiza uralensis Induce Differentiation of B16-F10 Melanoma Cells
title_short Prenylated Flavonoids from Roots of Glycyrrhiza uralensis Induce Differentiation of B16-F10 Melanoma Cells
title_sort prenylated flavonoids from roots of glycyrrhiza uralensis induce differentiation of b16-f10 melanoma cells
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6122034/
https://www.ncbi.nlm.nih.gov/pubmed/30115883
http://dx.doi.org/10.3390/ijms19082422
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