Preparation and characterization of norcantharidin liposomes modified with stearyl glycyrrhetinate

In the current study, norcantharidin (NCTD)-loaded liposomes (LIPs) modified with stearyl glycyrrhetinate (SG; SG-NCTD-LIP) were prepared by the ethanol injection method. To increase the drug encapsulation efficiency (EE), the formulation of NCTD-LIP was optimized by single factor test and orthogona...

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Detalles Bibliográficos
Autores principales: Zhu, Jing, Zhang, Wei, Wang, Dandan, Li, Suzhen, Wu, Wei
Formato: Online Artículo Texto
Lenguaje:English
Publicado: D.A. Spandidos 2018
Materias:
Articles
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6122258/
https://www.ncbi.nlm.nih.gov/pubmed/30186382
http://dx.doi.org/10.3892/etm.2018.6416
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author Zhu, Jing
Zhang, Wei
Wang, Dandan
Li, Suzhen
Wu, Wei
author_facet Zhu, Jing
Zhang, Wei
Wang, Dandan
Li, Suzhen
Wu, Wei
author_sort Zhu, Jing
collection PubMed
description In the current study, norcantharidin (NCTD)-loaded liposomes (LIPs) modified with stearyl glycyrrhetinate (SG; SG-NCTD-LIP) were prepared by the ethanol injection method. To increase the drug encapsulation efficiency (EE), the formulation of NCTD-LIP was optimized by single factor test and orthogonal design. The release of NCTD in vitro from SG-NCTD-LIP was evaluated by equilibrium dialysis. The cytotoxicity of SG-NCTD-LIP in HepG2 was investigated by MTT assay. The results revealed that the EE of liposomes was ~27.80%, the average SG-NCTD-LIP was 87.5 nm, the in vitro NCTD release from SG-NCTD-LIP was delayed compared with NCTD in solution and the drug-release kinetic followed a first-order model. MTT assays revealed increased cytotoxicity activity against HepG2 cells for SG-NCTD-LIP compared with free NCTD. In conclusion, SG-NCTD-LIP prepared in the present study may be a promising liposomal drug delivery system for anticancer drugs in liver-targeting therapy.
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spelling pubmed-61222582018-09-05 Preparation and characterization of norcantharidin liposomes modified with stearyl glycyrrhetinate Zhu, Jing Zhang, Wei Wang, Dandan Li, Suzhen Wu, Wei Exp Ther Med Articles In the current study, norcantharidin (NCTD)-loaded liposomes (LIPs) modified with stearyl glycyrrhetinate (SG; SG-NCTD-LIP) were prepared by the ethanol injection method. To increase the drug encapsulation efficiency (EE), the formulation of NCTD-LIP was optimized by single factor test and orthogonal design. The release of NCTD in vitro from SG-NCTD-LIP was evaluated by equilibrium dialysis. The cytotoxicity of SG-NCTD-LIP in HepG2 was investigated by MTT assay. The results revealed that the EE of liposomes was ~27.80%, the average SG-NCTD-LIP was 87.5 nm, the in vitro NCTD release from SG-NCTD-LIP was delayed compared with NCTD in solution and the drug-release kinetic followed a first-order model. MTT assays revealed increased cytotoxicity activity against HepG2 cells for SG-NCTD-LIP compared with free NCTD. In conclusion, SG-NCTD-LIP prepared in the present study may be a promising liposomal drug delivery system for anticancer drugs in liver-targeting therapy. D.A. Spandidos 2018-09 2018-07-06 /pmc/articles/PMC6122258/ /pubmed/30186382 http://dx.doi.org/10.3892/etm.2018.6416 Text en Copyright: © Zhu et al. This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License (https://creativecommons.org/licenses/by-nc-nd/4.0/) , which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.
spellingShingle Articles
Zhu, Jing
Zhang, Wei
Wang, Dandan
Li, Suzhen
Wu, Wei
Preparation and characterization of norcantharidin liposomes modified with stearyl glycyrrhetinate
title Preparation and characterization of norcantharidin liposomes modified with stearyl glycyrrhetinate
title_full Preparation and characterization of norcantharidin liposomes modified with stearyl glycyrrhetinate
title_fullStr Preparation and characterization of norcantharidin liposomes modified with stearyl glycyrrhetinate
title_full_unstemmed Preparation and characterization of norcantharidin liposomes modified with stearyl glycyrrhetinate
title_short Preparation and characterization of norcantharidin liposomes modified with stearyl glycyrrhetinate
title_sort preparation and characterization of norcantharidin liposomes modified with stearyl glycyrrhetinate
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6122258/
https://www.ncbi.nlm.nih.gov/pubmed/30186382
http://dx.doi.org/10.3892/etm.2018.6416
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