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Combination therapy with BMP-2 and psoralen enhances fracture healing in ovariectomized mice

The advantages of combining local delivery of bone morphogenetic protein (BMP)-2 with systemic or local anti-osteoporosis treatments have also been studied for enhancing osteoporotic fracture healing. The aim of the present study was to evaluate the effect of combination therapy with BMP-2 and psora...

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Autores principales: Huang, Kui, Wu, Guofeng, Zou, Ji, Peng, Songming
Formato: Online Artículo Texto
Lenguaje:English
Publicado: D.A. Spandidos 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6122261/
https://www.ncbi.nlm.nih.gov/pubmed/30186384
http://dx.doi.org/10.3892/etm.2018.6353
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author Huang, Kui
Wu, Guofeng
Zou, Ji
Peng, Songming
author_facet Huang, Kui
Wu, Guofeng
Zou, Ji
Peng, Songming
author_sort Huang, Kui
collection PubMed
description The advantages of combining local delivery of bone morphogenetic protein (BMP)-2 with systemic or local anti-osteoporosis treatments have also been studied for enhancing osteoporotic fracture healing. The aim of the present study was to evaluate the effect of combination therapy with BMP-2 and psoralen on fracture repair in ovariectomized mice. At 6 weeks after bilateral ovariectomy, mice (n=30) underwent unilateral transverse osteotomy on the femur and were divided into 3 groups. In the model group (n=10), animals were implanted with an absorbable collagen sponge (ACS) alone and administered physiological saline intragastrically (i.g.). In the recombinant human (rh)BMP-2 group (n=10), animals were implanted with an ACS loaded with 2.5 µg rhBMP-2 and administered physiological saline i.g. In the psoralen + rhBMP-2 group (n=10) animals were implanted with an ACS loaded with 2.5 µg rhBMP-2 and administered psoralen i.g. The mice were euthanized after 21 days and their fractured femurs were assessed by micro computed tomography, histological analysis and biomechanical testing. Furthermore, the serum of the animals was analyzed. Psoralen + rhBMP-2 exerted more beneficial effects on callus consolidation and biomechanical strength. In addition, increased bone-specific alkaline phosphatase levels and decreased C-terminal telopeptide of type-1 collagen were observed in the Psoralen + rhBMP-2 group. However, no difference in estrogen levels was detected between the groups. In conclusion, the present study demonstrated that in ovariectomized mice, combination of locally delivered BMP-2 and systemically administered psoralen improved bone healing compared with BMP-2 alone.
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spelling pubmed-61222612018-09-05 Combination therapy with BMP-2 and psoralen enhances fracture healing in ovariectomized mice Huang, Kui Wu, Guofeng Zou, Ji Peng, Songming Exp Ther Med Articles The advantages of combining local delivery of bone morphogenetic protein (BMP)-2 with systemic or local anti-osteoporosis treatments have also been studied for enhancing osteoporotic fracture healing. The aim of the present study was to evaluate the effect of combination therapy with BMP-2 and psoralen on fracture repair in ovariectomized mice. At 6 weeks after bilateral ovariectomy, mice (n=30) underwent unilateral transverse osteotomy on the femur and were divided into 3 groups. In the model group (n=10), animals were implanted with an absorbable collagen sponge (ACS) alone and administered physiological saline intragastrically (i.g.). In the recombinant human (rh)BMP-2 group (n=10), animals were implanted with an ACS loaded with 2.5 µg rhBMP-2 and administered physiological saline i.g. In the psoralen + rhBMP-2 group (n=10) animals were implanted with an ACS loaded with 2.5 µg rhBMP-2 and administered psoralen i.g. The mice were euthanized after 21 days and their fractured femurs were assessed by micro computed tomography, histological analysis and biomechanical testing. Furthermore, the serum of the animals was analyzed. Psoralen + rhBMP-2 exerted more beneficial effects on callus consolidation and biomechanical strength. In addition, increased bone-specific alkaline phosphatase levels and decreased C-terminal telopeptide of type-1 collagen were observed in the Psoralen + rhBMP-2 group. However, no difference in estrogen levels was detected between the groups. In conclusion, the present study demonstrated that in ovariectomized mice, combination of locally delivered BMP-2 and systemically administered psoralen improved bone healing compared with BMP-2 alone. D.A. Spandidos 2018-09 2018-06-26 /pmc/articles/PMC6122261/ /pubmed/30186384 http://dx.doi.org/10.3892/etm.2018.6353 Text en Copyright: © Huang et al. This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License (https://creativecommons.org/licenses/by-nc-nd/4.0/) , which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.
spellingShingle Articles
Huang, Kui
Wu, Guofeng
Zou, Ji
Peng, Songming
Combination therapy with BMP-2 and psoralen enhances fracture healing in ovariectomized mice
title Combination therapy with BMP-2 and psoralen enhances fracture healing in ovariectomized mice
title_full Combination therapy with BMP-2 and psoralen enhances fracture healing in ovariectomized mice
title_fullStr Combination therapy with BMP-2 and psoralen enhances fracture healing in ovariectomized mice
title_full_unstemmed Combination therapy with BMP-2 and psoralen enhances fracture healing in ovariectomized mice
title_short Combination therapy with BMP-2 and psoralen enhances fracture healing in ovariectomized mice
title_sort combination therapy with bmp-2 and psoralen enhances fracture healing in ovariectomized mice
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6122261/
https://www.ncbi.nlm.nih.gov/pubmed/30186384
http://dx.doi.org/10.3892/etm.2018.6353
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