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Expression, localisation and potential significance of aquaporins in benign and malignant human prostate tissue

BACKGROUND: To study the expression pattern, localisation and potential clinical significance of aquaporin water channels (AQP) both in prostate cancer (PC) cell lines and in benign and malignant human prostate tissue. METHODS: The AQP transcript and protein expression of HPrEC, LNCaP, DU-145 and PC...

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Autores principales: Bründl, Johannes, Wallinger, Sabine, Breyer, Johannes, Weber, Florian, Evert, Matthias, Georgopoulos, Nikolaos Theodoros, Rosenhammer, Bernd, Burger, Maximilian, Otto, Wolfgang, Rubenwolf, Peter
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6122723/
https://www.ncbi.nlm.nih.gov/pubmed/30176851
http://dx.doi.org/10.1186/s12894-018-0391-y
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author Bründl, Johannes
Wallinger, Sabine
Breyer, Johannes
Weber, Florian
Evert, Matthias
Georgopoulos, Nikolaos Theodoros
Rosenhammer, Bernd
Burger, Maximilian
Otto, Wolfgang
Rubenwolf, Peter
author_facet Bründl, Johannes
Wallinger, Sabine
Breyer, Johannes
Weber, Florian
Evert, Matthias
Georgopoulos, Nikolaos Theodoros
Rosenhammer, Bernd
Burger, Maximilian
Otto, Wolfgang
Rubenwolf, Peter
author_sort Bründl, Johannes
collection PubMed
description BACKGROUND: To study the expression pattern, localisation and potential clinical significance of aquaporin water channels (AQP) both in prostate cancer (PC) cell lines and in benign and malignant human prostate tissue. METHODS: The AQP transcript and protein expression of HPrEC, LNCaP, DU-145 and PC3 cell lines was investigated using reverse transcriptase polymerase chain reaction (RT-PCR) and immunofluorescence (IF) microscopy labelling. Immunohistochemistry (IHC) was performed to assess AQP protein expression in surgical specimens of benign prostatic hyperplasia as well as in PC. Tissue mRNA expression of AQPs was quantified by single-step reverse transcriptase quantitative polymerase chain reaction (qPCR). Relative gene expression was determined using the 40-ΔC(T) method and correlated to clinicopathological parameters. RESULTS: Transcripts of AQP 1, 3, 4, 7, 8, 10 and 11 were expressed in all four cell lines, while AQP 9 transcripts were not detected in malignant cell lines. IF microscopy confirmed AQP 3, 4, 5, 7 and 9 protein expression. IHC revealed highly heterogeneous AQP 3 protein expression in PC specimens, with a marked decrease in expression in tumours of increasing malignancy. Loss of AQP 9 was shown in PC specimens. mRNA expression of AQP3 was found to be negatively correlated to PSA levels (ρ = − 0.354; p = 0.013), D’Amico risk stratification (ρ = − 0.336; p = 0.012), ISUP grade (ρ = − 0.321; p = 0.017) and Gleason score (ρ = − 0.342; p = 0.011). CONCLUSIONS: This is the first study to systematically characterize human prostate cell lines, benign prostatic hyperplasia and PC in relation to all 13 members of the AQP family. Our results indicate the differential expression of several AQPs in benign and malignant prostate tissue. A significant correlation was observed between AQP 3 expression and tumour grade, with progressive loss in more malignant tumours. Taken together, AQPs may play a role in the progression of PC and AQP expression patterns may serve as a prognostic marker. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1186/s12894-018-0391-y) contains supplementary material, which is available to authorized users.
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spelling pubmed-61227232018-09-10 Expression, localisation and potential significance of aquaporins in benign and malignant human prostate tissue Bründl, Johannes Wallinger, Sabine Breyer, Johannes Weber, Florian Evert, Matthias Georgopoulos, Nikolaos Theodoros Rosenhammer, Bernd Burger, Maximilian Otto, Wolfgang Rubenwolf, Peter BMC Urol Research Article BACKGROUND: To study the expression pattern, localisation and potential clinical significance of aquaporin water channels (AQP) both in prostate cancer (PC) cell lines and in benign and malignant human prostate tissue. METHODS: The AQP transcript and protein expression of HPrEC, LNCaP, DU-145 and PC3 cell lines was investigated using reverse transcriptase polymerase chain reaction (RT-PCR) and immunofluorescence (IF) microscopy labelling. Immunohistochemistry (IHC) was performed to assess AQP protein expression in surgical specimens of benign prostatic hyperplasia as well as in PC. Tissue mRNA expression of AQPs was quantified by single-step reverse transcriptase quantitative polymerase chain reaction (qPCR). Relative gene expression was determined using the 40-ΔC(T) method and correlated to clinicopathological parameters. RESULTS: Transcripts of AQP 1, 3, 4, 7, 8, 10 and 11 were expressed in all four cell lines, while AQP 9 transcripts were not detected in malignant cell lines. IF microscopy confirmed AQP 3, 4, 5, 7 and 9 protein expression. IHC revealed highly heterogeneous AQP 3 protein expression in PC specimens, with a marked decrease in expression in tumours of increasing malignancy. Loss of AQP 9 was shown in PC specimens. mRNA expression of AQP3 was found to be negatively correlated to PSA levels (ρ = − 0.354; p = 0.013), D’Amico risk stratification (ρ = − 0.336; p = 0.012), ISUP grade (ρ = − 0.321; p = 0.017) and Gleason score (ρ = − 0.342; p = 0.011). CONCLUSIONS: This is the first study to systematically characterize human prostate cell lines, benign prostatic hyperplasia and PC in relation to all 13 members of the AQP family. Our results indicate the differential expression of several AQPs in benign and malignant prostate tissue. A significant correlation was observed between AQP 3 expression and tumour grade, with progressive loss in more malignant tumours. Taken together, AQPs may play a role in the progression of PC and AQP expression patterns may serve as a prognostic marker. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1186/s12894-018-0391-y) contains supplementary material, which is available to authorized users. BioMed Central 2018-09-03 /pmc/articles/PMC6122723/ /pubmed/30176851 http://dx.doi.org/10.1186/s12894-018-0391-y Text en © The Author(s). 2018 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research Article
Bründl, Johannes
Wallinger, Sabine
Breyer, Johannes
Weber, Florian
Evert, Matthias
Georgopoulos, Nikolaos Theodoros
Rosenhammer, Bernd
Burger, Maximilian
Otto, Wolfgang
Rubenwolf, Peter
Expression, localisation and potential significance of aquaporins in benign and malignant human prostate tissue
title Expression, localisation and potential significance of aquaporins in benign and malignant human prostate tissue
title_full Expression, localisation and potential significance of aquaporins in benign and malignant human prostate tissue
title_fullStr Expression, localisation and potential significance of aquaporins in benign and malignant human prostate tissue
title_full_unstemmed Expression, localisation and potential significance of aquaporins in benign and malignant human prostate tissue
title_short Expression, localisation and potential significance of aquaporins in benign and malignant human prostate tissue
title_sort expression, localisation and potential significance of aquaporins in benign and malignant human prostate tissue
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6122723/
https://www.ncbi.nlm.nih.gov/pubmed/30176851
http://dx.doi.org/10.1186/s12894-018-0391-y
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