Cargando…
Label-free toxicology screening of primary human mesenchymal cells and iPS-derived neurons
The high-throughput, label-free Corning Epic assay has applications in drug discovery, pharmacogenomics, cell receptor signaling, cell migration, and viral titration. The utility of Epic technology for biocompatibility testing has not been well established. In manufacturing of medical devices, in vi...
Autores principales: | , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2018
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6122932/ https://www.ncbi.nlm.nih.gov/pubmed/30180158 http://dx.doi.org/10.1371/journal.pone.0201671 |
_version_ | 1783352758054682624 |
---|---|
author | Piccinno, Maria Serena Petrachi, Tiziana Resca, Elisa Strusi, Valentina Bergamini, Valentina Mulas, Giuseppe Antonio Mari, Giorgio Dominici, Massimo Veronesi, Elena |
author_facet | Piccinno, Maria Serena Petrachi, Tiziana Resca, Elisa Strusi, Valentina Bergamini, Valentina Mulas, Giuseppe Antonio Mari, Giorgio Dominici, Massimo Veronesi, Elena |
author_sort | Piccinno, Maria Serena |
collection | PubMed |
description | The high-throughput, label-free Corning Epic assay has applications in drug discovery, pharmacogenomics, cell receptor signaling, cell migration, and viral titration. The utility of Epic technology for biocompatibility testing has not been well established. In manufacturing of medical devices, in vitro and in vivo biocompatibility assessments are mandatory, according to ISO 10993. The new medical device regulation MDR 745/2017 specifies that ex vivo assays that can closely recapitulate in vivo scenarios are needed to better evaluate biomedical devices. We propose herein that Epic technology—which enables detection of variations in cell mass distribution—is suitable for biocompatibility screening of compounds. In this study, we challenged primary human osteoblasts, endothelial cells, and neurons derived from induced pluripotent stem cells with specific concentrations of methyl methacrylate (MMA). Polymeric MMA has long been applied in cranioplasty, where it makes contact with multiple cell types. Application of Epic technology yielded real-time cytotoxicity profiles for all considered cell types. The results were compared with those from microscopic observation of the same culture plate used in the Epic analyses. The Epic assay should be further examined for its utility for cell biology, genomics, and proteomics companion assays. Our results suggest that Epic technology can be applied to biocompatibility evaluation of human cells in medical device development. |
format | Online Article Text |
id | pubmed-6122932 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-61229322018-09-16 Label-free toxicology screening of primary human mesenchymal cells and iPS-derived neurons Piccinno, Maria Serena Petrachi, Tiziana Resca, Elisa Strusi, Valentina Bergamini, Valentina Mulas, Giuseppe Antonio Mari, Giorgio Dominici, Massimo Veronesi, Elena PLoS One Research Article The high-throughput, label-free Corning Epic assay has applications in drug discovery, pharmacogenomics, cell receptor signaling, cell migration, and viral titration. The utility of Epic technology for biocompatibility testing has not been well established. In manufacturing of medical devices, in vitro and in vivo biocompatibility assessments are mandatory, according to ISO 10993. The new medical device regulation MDR 745/2017 specifies that ex vivo assays that can closely recapitulate in vivo scenarios are needed to better evaluate biomedical devices. We propose herein that Epic technology—which enables detection of variations in cell mass distribution—is suitable for biocompatibility screening of compounds. In this study, we challenged primary human osteoblasts, endothelial cells, and neurons derived from induced pluripotent stem cells with specific concentrations of methyl methacrylate (MMA). Polymeric MMA has long been applied in cranioplasty, where it makes contact with multiple cell types. Application of Epic technology yielded real-time cytotoxicity profiles for all considered cell types. The results were compared with those from microscopic observation of the same culture plate used in the Epic analyses. The Epic assay should be further examined for its utility for cell biology, genomics, and proteomics companion assays. Our results suggest that Epic technology can be applied to biocompatibility evaluation of human cells in medical device development. Public Library of Science 2018-09-04 /pmc/articles/PMC6122932/ /pubmed/30180158 http://dx.doi.org/10.1371/journal.pone.0201671 Text en © 2018 Piccinno et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Article Piccinno, Maria Serena Petrachi, Tiziana Resca, Elisa Strusi, Valentina Bergamini, Valentina Mulas, Giuseppe Antonio Mari, Giorgio Dominici, Massimo Veronesi, Elena Label-free toxicology screening of primary human mesenchymal cells and iPS-derived neurons |
title | Label-free toxicology screening of primary human mesenchymal cells and iPS-derived neurons |
title_full | Label-free toxicology screening of primary human mesenchymal cells and iPS-derived neurons |
title_fullStr | Label-free toxicology screening of primary human mesenchymal cells and iPS-derived neurons |
title_full_unstemmed | Label-free toxicology screening of primary human mesenchymal cells and iPS-derived neurons |
title_short | Label-free toxicology screening of primary human mesenchymal cells and iPS-derived neurons |
title_sort | label-free toxicology screening of primary human mesenchymal cells and ips-derived neurons |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6122932/ https://www.ncbi.nlm.nih.gov/pubmed/30180158 http://dx.doi.org/10.1371/journal.pone.0201671 |
work_keys_str_mv | AT piccinnomariaserena labelfreetoxicologyscreeningofprimaryhumanmesenchymalcellsandipsderivedneurons AT petrachitiziana labelfreetoxicologyscreeningofprimaryhumanmesenchymalcellsandipsderivedneurons AT rescaelisa labelfreetoxicologyscreeningofprimaryhumanmesenchymalcellsandipsderivedneurons AT strusivalentina labelfreetoxicologyscreeningofprimaryhumanmesenchymalcellsandipsderivedneurons AT bergaminivalentina labelfreetoxicologyscreeningofprimaryhumanmesenchymalcellsandipsderivedneurons AT mulasgiuseppeantonio labelfreetoxicologyscreeningofprimaryhumanmesenchymalcellsandipsderivedneurons AT marigiorgio labelfreetoxicologyscreeningofprimaryhumanmesenchymalcellsandipsderivedneurons AT dominicimassimo labelfreetoxicologyscreeningofprimaryhumanmesenchymalcellsandipsderivedneurons AT veronesielena labelfreetoxicologyscreeningofprimaryhumanmesenchymalcellsandipsderivedneurons |