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New Variant of Multidrug-Resistant Salmonella enterica Serovar Typhimurium Associated with Invasive Disease in Immunocompromised Patients in Vietnam

Nontyphoidal Salmonella (NTS), particularly Salmonella enterica serovar Typhimurium, is among the leading etiologic agents of bacterial enterocolitis globally and a well-characterized cause of invasive disease (iNTS) in sub-Saharan Africa. In contrast, S. Typhimurium is poorly defined in Southeast A...

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Autores principales: Mather, Alison E., Phuong, Tu Le Thi, Gao, Yunfeng, Clare, Simon, Mukhopadhyay, Subhankar, Goulding, David A., Hoang, Nhu Tran Do, Tuyen, Ha Thanh, Lan, Nguyen Phu Huong, Thompson, Corinne N., Trang, Nguyen Hoang Thu, Carrique-Mas, Juan, Tue, Ngo Tri, Campbell, James I., Rabaa, Maia A., Thanh, Duy Pham, Harcourt, Katherine, Hoa, Ngo Thi, Trung, Nguyen Vinh, Schultsz, Constance, Perron, Gabriel G., Coia, John E., Brown, Derek J., Okoro, Chinyere, Parkhill, Julian, Thomson, Nicholas R., Chau, Nguyen Van Vinh, Thwaites, Guy E., Maskell, Duncan J., Dougan, Gordon, Kenney, Linda J., Baker, Stephen
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Society for Microbiology 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6123440/
https://www.ncbi.nlm.nih.gov/pubmed/30181247
http://dx.doi.org/10.1128/mBio.01056-18
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author Mather, Alison E.
Phuong, Tu Le Thi
Gao, Yunfeng
Clare, Simon
Mukhopadhyay, Subhankar
Goulding, David A.
Hoang, Nhu Tran Do
Tuyen, Ha Thanh
Lan, Nguyen Phu Huong
Thompson, Corinne N.
Trang, Nguyen Hoang Thu
Carrique-Mas, Juan
Tue, Ngo Tri
Campbell, James I.
Rabaa, Maia A.
Thanh, Duy Pham
Harcourt, Katherine
Hoa, Ngo Thi
Trung, Nguyen Vinh
Schultsz, Constance
Perron, Gabriel G.
Coia, John E.
Brown, Derek J.
Okoro, Chinyere
Parkhill, Julian
Thomson, Nicholas R.
Chau, Nguyen Van Vinh
Thwaites, Guy E.
Maskell, Duncan J.
Dougan, Gordon
Kenney, Linda J.
Baker, Stephen
author_facet Mather, Alison E.
Phuong, Tu Le Thi
Gao, Yunfeng
Clare, Simon
Mukhopadhyay, Subhankar
Goulding, David A.
Hoang, Nhu Tran Do
Tuyen, Ha Thanh
Lan, Nguyen Phu Huong
Thompson, Corinne N.
Trang, Nguyen Hoang Thu
Carrique-Mas, Juan
Tue, Ngo Tri
Campbell, James I.
Rabaa, Maia A.
Thanh, Duy Pham
Harcourt, Katherine
Hoa, Ngo Thi
Trung, Nguyen Vinh
Schultsz, Constance
Perron, Gabriel G.
Coia, John E.
Brown, Derek J.
Okoro, Chinyere
Parkhill, Julian
Thomson, Nicholas R.
Chau, Nguyen Van Vinh
Thwaites, Guy E.
Maskell, Duncan J.
Dougan, Gordon
Kenney, Linda J.
Baker, Stephen
author_sort Mather, Alison E.
collection PubMed
description Nontyphoidal Salmonella (NTS), particularly Salmonella enterica serovar Typhimurium, is among the leading etiologic agents of bacterial enterocolitis globally and a well-characterized cause of invasive disease (iNTS) in sub-Saharan Africa. In contrast, S. Typhimurium is poorly defined in Southeast Asia, a known hot spot for zoonotic disease with a recently described burden of iNTS disease. Here, we aimed to add insight into the epidemiology and potential impact of zoonotic transfer and antimicrobial resistance (AMR) in S. Typhimurium associated with iNTS and enterocolitis in Vietnam. We performed whole-genome sequencing and phylogenetic reconstruction on 85 human (enterocolitis, carriage, and iNTS) and 113 animal S. Typhimurium isolates isolated in Vietnam. We found limited evidence for the zoonotic transmission of S. Typhimurium. However, we describe a chain of events where a pandemic monophasic variant of S. Typhimurium (serovar I:4,[5],12:i:− sequence type 34 [ST34]) has been introduced into Vietnam, reacquired a phase 2 flagellum, and acquired an IncHI2 multidrug-resistant plasmid. Notably, these novel biphasic ST34 S. Typhimurium variants were significantly associated with iNTS in Vietnamese HIV-infected patients. Our study represents the first characterization of novel iNTS organisms isolated outside sub-Saharan Africa and outlines a new pathway for the emergence of alternative Salmonella variants into susceptible human populations.
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spelling pubmed-61234402018-09-07 New Variant of Multidrug-Resistant Salmonella enterica Serovar Typhimurium Associated with Invasive Disease in Immunocompromised Patients in Vietnam Mather, Alison E. Phuong, Tu Le Thi Gao, Yunfeng Clare, Simon Mukhopadhyay, Subhankar Goulding, David A. Hoang, Nhu Tran Do Tuyen, Ha Thanh Lan, Nguyen Phu Huong Thompson, Corinne N. Trang, Nguyen Hoang Thu Carrique-Mas, Juan Tue, Ngo Tri Campbell, James I. Rabaa, Maia A. Thanh, Duy Pham Harcourt, Katherine Hoa, Ngo Thi Trung, Nguyen Vinh Schultsz, Constance Perron, Gabriel G. Coia, John E. Brown, Derek J. Okoro, Chinyere Parkhill, Julian Thomson, Nicholas R. Chau, Nguyen Van Vinh Thwaites, Guy E. Maskell, Duncan J. Dougan, Gordon Kenney, Linda J. Baker, Stephen mBio Research Article Nontyphoidal Salmonella (NTS), particularly Salmonella enterica serovar Typhimurium, is among the leading etiologic agents of bacterial enterocolitis globally and a well-characterized cause of invasive disease (iNTS) in sub-Saharan Africa. In contrast, S. Typhimurium is poorly defined in Southeast Asia, a known hot spot for zoonotic disease with a recently described burden of iNTS disease. Here, we aimed to add insight into the epidemiology and potential impact of zoonotic transfer and antimicrobial resistance (AMR) in S. Typhimurium associated with iNTS and enterocolitis in Vietnam. We performed whole-genome sequencing and phylogenetic reconstruction on 85 human (enterocolitis, carriage, and iNTS) and 113 animal S. Typhimurium isolates isolated in Vietnam. We found limited evidence for the zoonotic transmission of S. Typhimurium. However, we describe a chain of events where a pandemic monophasic variant of S. Typhimurium (serovar I:4,[5],12:i:− sequence type 34 [ST34]) has been introduced into Vietnam, reacquired a phase 2 flagellum, and acquired an IncHI2 multidrug-resistant plasmid. Notably, these novel biphasic ST34 S. Typhimurium variants were significantly associated with iNTS in Vietnamese HIV-infected patients. Our study represents the first characterization of novel iNTS organisms isolated outside sub-Saharan Africa and outlines a new pathway for the emergence of alternative Salmonella variants into susceptible human populations. American Society for Microbiology 2018-09-04 /pmc/articles/PMC6123440/ /pubmed/30181247 http://dx.doi.org/10.1128/mBio.01056-18 Text en Copyright © 2018 Mather et al. https://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Research Article
Mather, Alison E.
Phuong, Tu Le Thi
Gao, Yunfeng
Clare, Simon
Mukhopadhyay, Subhankar
Goulding, David A.
Hoang, Nhu Tran Do
Tuyen, Ha Thanh
Lan, Nguyen Phu Huong
Thompson, Corinne N.
Trang, Nguyen Hoang Thu
Carrique-Mas, Juan
Tue, Ngo Tri
Campbell, James I.
Rabaa, Maia A.
Thanh, Duy Pham
Harcourt, Katherine
Hoa, Ngo Thi
Trung, Nguyen Vinh
Schultsz, Constance
Perron, Gabriel G.
Coia, John E.
Brown, Derek J.
Okoro, Chinyere
Parkhill, Julian
Thomson, Nicholas R.
Chau, Nguyen Van Vinh
Thwaites, Guy E.
Maskell, Duncan J.
Dougan, Gordon
Kenney, Linda J.
Baker, Stephen
New Variant of Multidrug-Resistant Salmonella enterica Serovar Typhimurium Associated with Invasive Disease in Immunocompromised Patients in Vietnam
title New Variant of Multidrug-Resistant Salmonella enterica Serovar Typhimurium Associated with Invasive Disease in Immunocompromised Patients in Vietnam
title_full New Variant of Multidrug-Resistant Salmonella enterica Serovar Typhimurium Associated with Invasive Disease in Immunocompromised Patients in Vietnam
title_fullStr New Variant of Multidrug-Resistant Salmonella enterica Serovar Typhimurium Associated with Invasive Disease in Immunocompromised Patients in Vietnam
title_full_unstemmed New Variant of Multidrug-Resistant Salmonella enterica Serovar Typhimurium Associated with Invasive Disease in Immunocompromised Patients in Vietnam
title_short New Variant of Multidrug-Resistant Salmonella enterica Serovar Typhimurium Associated with Invasive Disease in Immunocompromised Patients in Vietnam
title_sort new variant of multidrug-resistant salmonella enterica serovar typhimurium associated with invasive disease in immunocompromised patients in vietnam
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6123440/
https://www.ncbi.nlm.nih.gov/pubmed/30181247
http://dx.doi.org/10.1128/mBio.01056-18
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