Cargando…

Distinct Pathway of Human T-Cell Leukemia Virus Type 1 Gag Punctum Biogenesis Provides New Insights into Enveloped Virus Assembly

The assembly of virus particles is a crucial aspect of virus spread. For retroviruses, the Gag polyprotein is the key driver for virus particle assembly. In order to produce progeny virus, once Gag is translated, it must translocate from the location in the cytoplasm where it is synthesized to the p...

Descripción completa

Detalles Bibliográficos
Autores principales: Eichorst, John P., Chen, Yan, Mueller, Joachim D., Mansky, Louis M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Society for Microbiology 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6123448/
https://www.ncbi.nlm.nih.gov/pubmed/30181245
http://dx.doi.org/10.1128/mBio.00758-18
_version_ 1783352841400745984
author Eichorst, John P.
Chen, Yan
Mueller, Joachim D.
Mansky, Louis M.
author_facet Eichorst, John P.
Chen, Yan
Mueller, Joachim D.
Mansky, Louis M.
author_sort Eichorst, John P.
collection PubMed
description The assembly of virus particles is a crucial aspect of virus spread. For retroviruses, the Gag polyprotein is the key driver for virus particle assembly. In order to produce progeny virus, once Gag is translated, it must translocate from the location in the cytoplasm where it is synthesized to the plasma membrane and form an oligomeric lattice that results in Gag puncta. The biogenesis of mature Gag puncta can trigger the budding process, resulting in virus particle production. While some aspects of the dynamics of Gag oligomerization and particle biogenesis have been observed with human immunodeficiency virus type 1 (HIV-1), the process of Gag punctum biogenesis remains poorly understood, particularly for other retroviruses. Here, we have conducted the most detailed studies thus far on Gag punctum biogenesis for human T-cell leukemia virus type 1 (HTLV-1). Using mEos2 photoconvertible fluorescent proteins and total internal reflection fluorescence microscopy (TIRF), we have found that HTLV-1 Gag was recruited to Gag puncta primarily from the plasma membrane. This was in stark contrast to HIV-1 Gag, which was recruited from the cytoplasm. These observations imply fundamental differences among retroviruses regarding the orchestration of Gag punctum biogenesis, which has important general implications for enveloped virus particle assembly.
format Online
Article
Text
id pubmed-6123448
institution National Center for Biotechnology Information
language English
publishDate 2018
publisher American Society for Microbiology
record_format MEDLINE/PubMed
spelling pubmed-61234482018-09-07 Distinct Pathway of Human T-Cell Leukemia Virus Type 1 Gag Punctum Biogenesis Provides New Insights into Enveloped Virus Assembly Eichorst, John P. Chen, Yan Mueller, Joachim D. Mansky, Louis M. mBio Research Article The assembly of virus particles is a crucial aspect of virus spread. For retroviruses, the Gag polyprotein is the key driver for virus particle assembly. In order to produce progeny virus, once Gag is translated, it must translocate from the location in the cytoplasm where it is synthesized to the plasma membrane and form an oligomeric lattice that results in Gag puncta. The biogenesis of mature Gag puncta can trigger the budding process, resulting in virus particle production. While some aspects of the dynamics of Gag oligomerization and particle biogenesis have been observed with human immunodeficiency virus type 1 (HIV-1), the process of Gag punctum biogenesis remains poorly understood, particularly for other retroviruses. Here, we have conducted the most detailed studies thus far on Gag punctum biogenesis for human T-cell leukemia virus type 1 (HTLV-1). Using mEos2 photoconvertible fluorescent proteins and total internal reflection fluorescence microscopy (TIRF), we have found that HTLV-1 Gag was recruited to Gag puncta primarily from the plasma membrane. This was in stark contrast to HIV-1 Gag, which was recruited from the cytoplasm. These observations imply fundamental differences among retroviruses regarding the orchestration of Gag punctum biogenesis, which has important general implications for enveloped virus particle assembly. American Society for Microbiology 2018-09-04 /pmc/articles/PMC6123448/ /pubmed/30181245 http://dx.doi.org/10.1128/mBio.00758-18 Text en Copyright © 2018 Eichorst et al. https://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Research Article
Eichorst, John P.
Chen, Yan
Mueller, Joachim D.
Mansky, Louis M.
Distinct Pathway of Human T-Cell Leukemia Virus Type 1 Gag Punctum Biogenesis Provides New Insights into Enveloped Virus Assembly
title Distinct Pathway of Human T-Cell Leukemia Virus Type 1 Gag Punctum Biogenesis Provides New Insights into Enveloped Virus Assembly
title_full Distinct Pathway of Human T-Cell Leukemia Virus Type 1 Gag Punctum Biogenesis Provides New Insights into Enveloped Virus Assembly
title_fullStr Distinct Pathway of Human T-Cell Leukemia Virus Type 1 Gag Punctum Biogenesis Provides New Insights into Enveloped Virus Assembly
title_full_unstemmed Distinct Pathway of Human T-Cell Leukemia Virus Type 1 Gag Punctum Biogenesis Provides New Insights into Enveloped Virus Assembly
title_short Distinct Pathway of Human T-Cell Leukemia Virus Type 1 Gag Punctum Biogenesis Provides New Insights into Enveloped Virus Assembly
title_sort distinct pathway of human t-cell leukemia virus type 1 gag punctum biogenesis provides new insights into enveloped virus assembly
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6123448/
https://www.ncbi.nlm.nih.gov/pubmed/30181245
http://dx.doi.org/10.1128/mBio.00758-18
work_keys_str_mv AT eichorstjohnp distinctpathwayofhumantcellleukemiavirustype1gagpunctumbiogenesisprovidesnewinsightsintoenvelopedvirusassembly
AT chenyan distinctpathwayofhumantcellleukemiavirustype1gagpunctumbiogenesisprovidesnewinsightsintoenvelopedvirusassembly
AT muellerjoachimd distinctpathwayofhumantcellleukemiavirustype1gagpunctumbiogenesisprovidesnewinsightsintoenvelopedvirusassembly
AT manskylouism distinctpathwayofhumantcellleukemiavirustype1gagpunctumbiogenesisprovidesnewinsightsintoenvelopedvirusassembly