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Increasing Cas9-mediated homology-directed repair efficiency through covalent tethering of DNA repair template

The CRISPR-Cas9 system is a powerful genome-editing tool in which a guide RNA targets Cas9 to a site in the genome, where the Cas9 nuclease then induces a double-stranded break (DSB). The potential of CRISPR-Cas9 to deliver precise genome editing is hindered by the low efficiency of homology-directe...

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Detalles Bibliográficos
Autores principales:	Aird, Eric J., Lovendahl, Klaus N., St. Martin, Amber, Harris, Reuben S., Gordon, Wendy R.
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Nature Publishing Group UK 2018
Materias:	Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6123678/ https://www.ncbi.nlm.nih.gov/pubmed/30271937 http://dx.doi.org/10.1038/s42003-018-0054-2

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