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Store-operated Ca(2+) entry is activated by every action potential in skeletal muscle

Store-operated calcium (Ca(2+)) entry (SOCE) in skeletal muscle is rapidly activated across the tubular system during direct activation of Ca(2+) release. The tubular system is the invagination of the plasma membrane that forms junctions with the sarcoplasmic reticulum (SR) where STIM1, Orai1 and ry...

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Autores principales: Koenig, Xaver, Choi, Rocky H, Launikonis, Bradley S
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6123720/
https://www.ncbi.nlm.nih.gov/pubmed/30271917
http://dx.doi.org/10.1038/s42003-018-0033-7
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author Koenig, Xaver
Choi, Rocky H
Launikonis, Bradley S
author_facet Koenig, Xaver
Choi, Rocky H
Launikonis, Bradley S
author_sort Koenig, Xaver
collection PubMed
description Store-operated calcium (Ca(2+)) entry (SOCE) in skeletal muscle is rapidly activated across the tubular system during direct activation of Ca(2+) release. The tubular system is the invagination of the plasma membrane that forms junctions with the sarcoplasmic reticulum (SR) where STIM1, Orai1 and ryanodine receptors are found. The physiological activation of SOCE in muscle is not defined, thus clouding its physiological role. Here we show that the magnitude of a phasic tubular system Ca(2+) influx is dependent on SR Ca(2+) depletion magnitude, and define this as SOCE. Consistent with SOCE, the influx was resistant to nifedipine and BayK8644, and silenced by inhibition of SR Ca(2+) release during excitation. The SOCE transient was shaped by action potential frequency and SR Ca(2+) pump activity. Our results show that SOCE in skeletal muscle acts as an immediate counter-flux to Ca(2+) loss across the tubular system during excitation-contraction coupling.
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spelling pubmed-61237202018-09-28 Store-operated Ca(2+) entry is activated by every action potential in skeletal muscle Koenig, Xaver Choi, Rocky H Launikonis, Bradley S Commun Biol Article Store-operated calcium (Ca(2+)) entry (SOCE) in skeletal muscle is rapidly activated across the tubular system during direct activation of Ca(2+) release. The tubular system is the invagination of the plasma membrane that forms junctions with the sarcoplasmic reticulum (SR) where STIM1, Orai1 and ryanodine receptors are found. The physiological activation of SOCE in muscle is not defined, thus clouding its physiological role. Here we show that the magnitude of a phasic tubular system Ca(2+) influx is dependent on SR Ca(2+) depletion magnitude, and define this as SOCE. Consistent with SOCE, the influx was resistant to nifedipine and BayK8644, and silenced by inhibition of SR Ca(2+) release during excitation. The SOCE transient was shaped by action potential frequency and SR Ca(2+) pump activity. Our results show that SOCE in skeletal muscle acts as an immediate counter-flux to Ca(2+) loss across the tubular system during excitation-contraction coupling. Nature Publishing Group UK 2018-04-19 /pmc/articles/PMC6123720/ /pubmed/30271917 http://dx.doi.org/10.1038/s42003-018-0033-7 Text en © The Author(s) 2018 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Koenig, Xaver
Choi, Rocky H
Launikonis, Bradley S
Store-operated Ca(2+) entry is activated by every action potential in skeletal muscle
title Store-operated Ca(2+) entry is activated by every action potential in skeletal muscle
title_full Store-operated Ca(2+) entry is activated by every action potential in skeletal muscle
title_fullStr Store-operated Ca(2+) entry is activated by every action potential in skeletal muscle
title_full_unstemmed Store-operated Ca(2+) entry is activated by every action potential in skeletal muscle
title_short Store-operated Ca(2+) entry is activated by every action potential in skeletal muscle
title_sort store-operated ca(2+) entry is activated by every action potential in skeletal muscle
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6123720/
https://www.ncbi.nlm.nih.gov/pubmed/30271917
http://dx.doi.org/10.1038/s42003-018-0033-7
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