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Overexpression of a rice BAHD acyltransferase gene in switchgrass (Panicum virgatum L.) enhances saccharification
BACKGROUND: Switchgrass (Panicum virgatum L.) is a promising bioenergy feedstock because it can be grown on marginal land and produces abundant biomass. Recalcitrance of the lignocellulosic components of the switchgrass cell wall to enzymatic degradation into simple sugars impedes efficient biofuel...
Autores principales: | , , , , , , , , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6123914/ https://www.ncbi.nlm.nih.gov/pubmed/30180895 http://dx.doi.org/10.1186/s12896-018-0464-8 |
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author | Li, Guotian Jones, Kyle C. Eudes, Aymerick Pidatala, Venkataramana R. Sun, Jian Xu, Feng Zhang, Chengcheng Wei, Tong Jain, Rashmi Birdseye, Devon Canlas, Patrick E. Baidoo, Edward E. K. Duong, Phat Q. Sharma, Manoj K. Singh, Seema Ruan, Deling Keasling, Jay D. Mortimer, Jenny C. Loqué, Dominique Bartley, Laura E. Scheller, Henrik V. Ronald, Pamela C. |
author_facet | Li, Guotian Jones, Kyle C. Eudes, Aymerick Pidatala, Venkataramana R. Sun, Jian Xu, Feng Zhang, Chengcheng Wei, Tong Jain, Rashmi Birdseye, Devon Canlas, Patrick E. Baidoo, Edward E. K. Duong, Phat Q. Sharma, Manoj K. Singh, Seema Ruan, Deling Keasling, Jay D. Mortimer, Jenny C. Loqué, Dominique Bartley, Laura E. Scheller, Henrik V. Ronald, Pamela C. |
author_sort | Li, Guotian |
collection | PubMed |
description | BACKGROUND: Switchgrass (Panicum virgatum L.) is a promising bioenergy feedstock because it can be grown on marginal land and produces abundant biomass. Recalcitrance of the lignocellulosic components of the switchgrass cell wall to enzymatic degradation into simple sugars impedes efficient biofuel production. We previously demonstrated that overexpression of OsAT10, a BAHD acyltransferase gene, enhances saccharification efficiency in rice. RESULTS: Here we show that overexpression of the rice OsAT10 gene in switchgrass decreased the levels of cell wall-bound ferulic acid (FA) in green leaf tissues and to a lesser extent in senesced tissues, and significantly increased levels of cell wall-bound p-coumaric acid (p-CA) in green leaves but decreased its level in senesced tissues of the T(0) plants under greenhouse conditions. The engineered switchgrass lines exhibit an approximate 40% increase in saccharification efficiency in green tissues and a 30% increase in senesced tissues. CONCLUSION: Our study demonstrates that overexpression of OsAT10, a rice BAHD acyltransferase gene, enhances saccharification of lignocellulosic biomass in switchgrass. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1186/s12896-018-0464-8) contains supplementary material, which is available to authorized users. |
format | Online Article Text |
id | pubmed-6123914 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-61239142018-09-10 Overexpression of a rice BAHD acyltransferase gene in switchgrass (Panicum virgatum L.) enhances saccharification Li, Guotian Jones, Kyle C. Eudes, Aymerick Pidatala, Venkataramana R. Sun, Jian Xu, Feng Zhang, Chengcheng Wei, Tong Jain, Rashmi Birdseye, Devon Canlas, Patrick E. Baidoo, Edward E. K. Duong, Phat Q. Sharma, Manoj K. Singh, Seema Ruan, Deling Keasling, Jay D. Mortimer, Jenny C. Loqué, Dominique Bartley, Laura E. Scheller, Henrik V. Ronald, Pamela C. BMC Biotechnol Research Article BACKGROUND: Switchgrass (Panicum virgatum L.) is a promising bioenergy feedstock because it can be grown on marginal land and produces abundant biomass. Recalcitrance of the lignocellulosic components of the switchgrass cell wall to enzymatic degradation into simple sugars impedes efficient biofuel production. We previously demonstrated that overexpression of OsAT10, a BAHD acyltransferase gene, enhances saccharification efficiency in rice. RESULTS: Here we show that overexpression of the rice OsAT10 gene in switchgrass decreased the levels of cell wall-bound ferulic acid (FA) in green leaf tissues and to a lesser extent in senesced tissues, and significantly increased levels of cell wall-bound p-coumaric acid (p-CA) in green leaves but decreased its level in senesced tissues of the T(0) plants under greenhouse conditions. The engineered switchgrass lines exhibit an approximate 40% increase in saccharification efficiency in green tissues and a 30% increase in senesced tissues. CONCLUSION: Our study demonstrates that overexpression of OsAT10, a rice BAHD acyltransferase gene, enhances saccharification of lignocellulosic biomass in switchgrass. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1186/s12896-018-0464-8) contains supplementary material, which is available to authorized users. BioMed Central 2018-09-04 /pmc/articles/PMC6123914/ /pubmed/30180895 http://dx.doi.org/10.1186/s12896-018-0464-8 Text en © The Author(s). 2018 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated. |
spellingShingle | Research Article Li, Guotian Jones, Kyle C. Eudes, Aymerick Pidatala, Venkataramana R. Sun, Jian Xu, Feng Zhang, Chengcheng Wei, Tong Jain, Rashmi Birdseye, Devon Canlas, Patrick E. Baidoo, Edward E. K. Duong, Phat Q. Sharma, Manoj K. Singh, Seema Ruan, Deling Keasling, Jay D. Mortimer, Jenny C. Loqué, Dominique Bartley, Laura E. Scheller, Henrik V. Ronald, Pamela C. Overexpression of a rice BAHD acyltransferase gene in switchgrass (Panicum virgatum L.) enhances saccharification |
title | Overexpression of a rice BAHD acyltransferase gene in switchgrass (Panicum virgatum L.) enhances saccharification |
title_full | Overexpression of a rice BAHD acyltransferase gene in switchgrass (Panicum virgatum L.) enhances saccharification |
title_fullStr | Overexpression of a rice BAHD acyltransferase gene in switchgrass (Panicum virgatum L.) enhances saccharification |
title_full_unstemmed | Overexpression of a rice BAHD acyltransferase gene in switchgrass (Panicum virgatum L.) enhances saccharification |
title_short | Overexpression of a rice BAHD acyltransferase gene in switchgrass (Panicum virgatum L.) enhances saccharification |
title_sort | overexpression of a rice bahd acyltransferase gene in switchgrass (panicum virgatum l.) enhances saccharification |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6123914/ https://www.ncbi.nlm.nih.gov/pubmed/30180895 http://dx.doi.org/10.1186/s12896-018-0464-8 |
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