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Increased ROS production and DNA damage in monocytes are biomarkers of aging and atherosclerosis
BACKGROUND: New evidence demonstrates that aging and dyslipidemia are closely associated with oxidative stress, DNA damage and apoptosis in some cells and extravascular tissues. However, in monocytes, which are naturally involved in progression and/or resolution of plaque in atherosclerosis, this co...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6123971/ https://www.ncbi.nlm.nih.gov/pubmed/30185234 http://dx.doi.org/10.1186/s40659-018-0182-7 |
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author | Jacinto, Thais A. Meireles, Giselle S. Dias, Ananda T. Aires, Rafaela Porto, Marcella L. Gava, Agata L. Vasquez, Elisardo C. Pereira, Thiago Melo C. Campagnaro, Bianca P. Meyrelles, Silvana S. |
author_facet | Jacinto, Thais A. Meireles, Giselle S. Dias, Ananda T. Aires, Rafaela Porto, Marcella L. Gava, Agata L. Vasquez, Elisardo C. Pereira, Thiago Melo C. Campagnaro, Bianca P. Meyrelles, Silvana S. |
author_sort | Jacinto, Thais A. |
collection | PubMed |
description | BACKGROUND: New evidence demonstrates that aging and dyslipidemia are closely associated with oxidative stress, DNA damage and apoptosis in some cells and extravascular tissues. However, in monocytes, which are naturally involved in progression and/or resolution of plaque in atherosclerosis, this concurrence has not yet been fully investigated. In this study, we evaluated the influence of aging and hypercholesterolemia on serum pro-inflammatory cytokines, oxidative stress, DNA damage and apoptosis in monocytes from apolipoprotein E-deficient (apoE(−/−)) mice compared with age-matched wild-type C57BL/6 (WT) mice. Experiments were performed in young (2-months) and in old (18-months) male wild-type (WT) and apoE(−/−) mice. RESULTS: Besides the expected differences in serum lipid profile and plaque formation, we observed that atherosclerotic mice exhibited a significant increase in monocytosis and in serum levels of pro-inflammatory cytokines compared to WT mice. Moreover, it was observed that the overproduction of ROS, led to an increased DNA fragmentation and, consequently, apoptosis in monocytes from normocholesterolemic old mice, which was aggravated in age-matched atherosclerotic mice. CONCLUSIONS: In this study, we demonstrate that a pro-inflammatory systemic status is associated with an impairment of functionality of monocytes during aging and that these parameters are fundamental extra-arterial contributors to the aggravation of atherosclerosis. The present data open new avenues for the development of future strategies with the purpose of treating atherosclerosis. |
format | Online Article Text |
id | pubmed-6123971 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-61239712018-09-10 Increased ROS production and DNA damage in monocytes are biomarkers of aging and atherosclerosis Jacinto, Thais A. Meireles, Giselle S. Dias, Ananda T. Aires, Rafaela Porto, Marcella L. Gava, Agata L. Vasquez, Elisardo C. Pereira, Thiago Melo C. Campagnaro, Bianca P. Meyrelles, Silvana S. Biol Res Research Article BACKGROUND: New evidence demonstrates that aging and dyslipidemia are closely associated with oxidative stress, DNA damage and apoptosis in some cells and extravascular tissues. However, in monocytes, which are naturally involved in progression and/or resolution of plaque in atherosclerosis, this concurrence has not yet been fully investigated. In this study, we evaluated the influence of aging and hypercholesterolemia on serum pro-inflammatory cytokines, oxidative stress, DNA damage and apoptosis in monocytes from apolipoprotein E-deficient (apoE(−/−)) mice compared with age-matched wild-type C57BL/6 (WT) mice. Experiments were performed in young (2-months) and in old (18-months) male wild-type (WT) and apoE(−/−) mice. RESULTS: Besides the expected differences in serum lipid profile and plaque formation, we observed that atherosclerotic mice exhibited a significant increase in monocytosis and in serum levels of pro-inflammatory cytokines compared to WT mice. Moreover, it was observed that the overproduction of ROS, led to an increased DNA fragmentation and, consequently, apoptosis in monocytes from normocholesterolemic old mice, which was aggravated in age-matched atherosclerotic mice. CONCLUSIONS: In this study, we demonstrate that a pro-inflammatory systemic status is associated with an impairment of functionality of monocytes during aging and that these parameters are fundamental extra-arterial contributors to the aggravation of atherosclerosis. The present data open new avenues for the development of future strategies with the purpose of treating atherosclerosis. BioMed Central 2018-09-05 /pmc/articles/PMC6123971/ /pubmed/30185234 http://dx.doi.org/10.1186/s40659-018-0182-7 Text en © The Author(s) 2018 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated. |
spellingShingle | Research Article Jacinto, Thais A. Meireles, Giselle S. Dias, Ananda T. Aires, Rafaela Porto, Marcella L. Gava, Agata L. Vasquez, Elisardo C. Pereira, Thiago Melo C. Campagnaro, Bianca P. Meyrelles, Silvana S. Increased ROS production and DNA damage in monocytes are biomarkers of aging and atherosclerosis |
title | Increased ROS production and DNA damage in monocytes are biomarkers of aging and atherosclerosis |
title_full | Increased ROS production and DNA damage in monocytes are biomarkers of aging and atherosclerosis |
title_fullStr | Increased ROS production and DNA damage in monocytes are biomarkers of aging and atherosclerosis |
title_full_unstemmed | Increased ROS production and DNA damage in monocytes are biomarkers of aging and atherosclerosis |
title_short | Increased ROS production and DNA damage in monocytes are biomarkers of aging and atherosclerosis |
title_sort | increased ros production and dna damage in monocytes are biomarkers of aging and atherosclerosis |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6123971/ https://www.ncbi.nlm.nih.gov/pubmed/30185234 http://dx.doi.org/10.1186/s40659-018-0182-7 |
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