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Biomimetic oyster shell–replicated topography alters the behaviour of human skeletal stem cells
The regenerative potential of skeletal stem cells provides an attractive prospect to generate bone tissue needed for musculoskeletal reparation. A central issue remains efficacious, controlled cell differentiation strategies to aid progression of cell therapies to the clinic. The nacre surface from...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
SAGE Publications
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6124183/ https://www.ncbi.nlm.nih.gov/pubmed/30202512 http://dx.doi.org/10.1177/2041731418794007 |
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author | Waddell, Shona J de Andrés, María C Tsimbouri, Penelope M Alakpa, Enateri V Cusack, Maggie Dalby, Matthew J Oreffo, Richard OC |
author_facet | Waddell, Shona J de Andrés, María C Tsimbouri, Penelope M Alakpa, Enateri V Cusack, Maggie Dalby, Matthew J Oreffo, Richard OC |
author_sort | Waddell, Shona J |
collection | PubMed |
description | The regenerative potential of skeletal stem cells provides an attractive prospect to generate bone tissue needed for musculoskeletal reparation. A central issue remains efficacious, controlled cell differentiation strategies to aid progression of cell therapies to the clinic. The nacre surface from Pinctada maxima shells is known to enhance bone formation. However, to date, there is a paucity of information on the role of the topography of P. maxima surfaces, nacre and prism. To investigate this, nacre and prism topographical features were replicated onto polycaprolactone and skeletal stem cell behaviour on the surfaces studied. Skeletal stem cells on nacre surfaces exhibited an increase in cell area, increase in expression of osteogenic markers ALP (p < 0.05) and OCN (p < 0.01) and increased metabolite intensity (p < 0.05), indicating a role of nacre surface to induce osteogenic differentiation, while on prism surfaces, skeletal stem cells did not show alterations in cell area or osteogenic marker expression and a decrease in metabolite intensity (p < 0.05), demonstrating a distinct role for the prism surface, with the potential to maintain the skeletal stem cell phenotype. |
format | Online Article Text |
id | pubmed-6124183 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | SAGE Publications |
record_format | MEDLINE/PubMed |
spelling | pubmed-61241832018-09-10 Biomimetic oyster shell–replicated topography alters the behaviour of human skeletal stem cells Waddell, Shona J de Andrés, María C Tsimbouri, Penelope M Alakpa, Enateri V Cusack, Maggie Dalby, Matthew J Oreffo, Richard OC J Tissue Eng Cell Engineering at the Micro/Nanoscale – dedicated to Prof Adam Curtis The regenerative potential of skeletal stem cells provides an attractive prospect to generate bone tissue needed for musculoskeletal reparation. A central issue remains efficacious, controlled cell differentiation strategies to aid progression of cell therapies to the clinic. The nacre surface from Pinctada maxima shells is known to enhance bone formation. However, to date, there is a paucity of information on the role of the topography of P. maxima surfaces, nacre and prism. To investigate this, nacre and prism topographical features were replicated onto polycaprolactone and skeletal stem cell behaviour on the surfaces studied. Skeletal stem cells on nacre surfaces exhibited an increase in cell area, increase in expression of osteogenic markers ALP (p < 0.05) and OCN (p < 0.01) and increased metabolite intensity (p < 0.05), indicating a role of nacre surface to induce osteogenic differentiation, while on prism surfaces, skeletal stem cells did not show alterations in cell area or osteogenic marker expression and a decrease in metabolite intensity (p < 0.05), demonstrating a distinct role for the prism surface, with the potential to maintain the skeletal stem cell phenotype. SAGE Publications 2018-09-04 /pmc/articles/PMC6124183/ /pubmed/30202512 http://dx.doi.org/10.1177/2041731418794007 Text en © The Author(s) 2018 http://www.creativecommons.org/licenses/by-nc/4.0/ This article is distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 License (http://www.creativecommons.org/licenses/by-nc/4.0/) which permits non-commercial use, reproduction and distribution of the work without further permission provided the original work is attributed as specified on the SAGE and Open Access pages (https://us.sagepub.com/en-us/nam/open-access-at-sage). |
spellingShingle | Cell Engineering at the Micro/Nanoscale – dedicated to Prof Adam Curtis Waddell, Shona J de Andrés, María C Tsimbouri, Penelope M Alakpa, Enateri V Cusack, Maggie Dalby, Matthew J Oreffo, Richard OC Biomimetic oyster shell–replicated topography alters the behaviour of human skeletal stem cells |
title | Biomimetic oyster shell–replicated topography alters the behaviour of
human skeletal stem cells |
title_full | Biomimetic oyster shell–replicated topography alters the behaviour of
human skeletal stem cells |
title_fullStr | Biomimetic oyster shell–replicated topography alters the behaviour of
human skeletal stem cells |
title_full_unstemmed | Biomimetic oyster shell–replicated topography alters the behaviour of
human skeletal stem cells |
title_short | Biomimetic oyster shell–replicated topography alters the behaviour of
human skeletal stem cells |
title_sort | biomimetic oyster shell–replicated topography alters the behaviour of
human skeletal stem cells |
topic | Cell Engineering at the Micro/Nanoscale – dedicated to Prof Adam Curtis |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6124183/ https://www.ncbi.nlm.nih.gov/pubmed/30202512 http://dx.doi.org/10.1177/2041731418794007 |
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