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Biomimetic oyster shell–replicated topography alters the behaviour of human skeletal stem cells

The regenerative potential of skeletal stem cells provides an attractive prospect to generate bone tissue needed for musculoskeletal reparation. A central issue remains efficacious, controlled cell differentiation strategies to aid progression of cell therapies to the clinic. The nacre surface from...

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Autores principales: Waddell, Shona J, de Andrés, María C, Tsimbouri, Penelope M, Alakpa, Enateri V, Cusack, Maggie, Dalby, Matthew J, Oreffo, Richard OC
Formato: Online Artículo Texto
Lenguaje:English
Publicado: SAGE Publications 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6124183/
https://www.ncbi.nlm.nih.gov/pubmed/30202512
http://dx.doi.org/10.1177/2041731418794007
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author Waddell, Shona J
de Andrés, María C
Tsimbouri, Penelope M
Alakpa, Enateri V
Cusack, Maggie
Dalby, Matthew J
Oreffo, Richard OC
author_facet Waddell, Shona J
de Andrés, María C
Tsimbouri, Penelope M
Alakpa, Enateri V
Cusack, Maggie
Dalby, Matthew J
Oreffo, Richard OC
author_sort Waddell, Shona J
collection PubMed
description The regenerative potential of skeletal stem cells provides an attractive prospect to generate bone tissue needed for musculoskeletal reparation. A central issue remains efficacious, controlled cell differentiation strategies to aid progression of cell therapies to the clinic. The nacre surface from Pinctada maxima shells is known to enhance bone formation. However, to date, there is a paucity of information on the role of the topography of P. maxima surfaces, nacre and prism. To investigate this, nacre and prism topographical features were replicated onto polycaprolactone and skeletal stem cell behaviour on the surfaces studied. Skeletal stem cells on nacre surfaces exhibited an increase in cell area, increase in expression of osteogenic markers ALP (p < 0.05) and OCN (p < 0.01) and increased metabolite intensity (p < 0.05), indicating a role of nacre surface to induce osteogenic differentiation, while on prism surfaces, skeletal stem cells did not show alterations in cell area or osteogenic marker expression and a decrease in metabolite intensity (p < 0.05), demonstrating a distinct role for the prism surface, with the potential to maintain the skeletal stem cell phenotype.
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spelling pubmed-61241832018-09-10 Biomimetic oyster shell–replicated topography alters the behaviour of human skeletal stem cells Waddell, Shona J de Andrés, María C Tsimbouri, Penelope M Alakpa, Enateri V Cusack, Maggie Dalby, Matthew J Oreffo, Richard OC J Tissue Eng Cell Engineering at the Micro/Nanoscale – dedicated to Prof Adam Curtis The regenerative potential of skeletal stem cells provides an attractive prospect to generate bone tissue needed for musculoskeletal reparation. A central issue remains efficacious, controlled cell differentiation strategies to aid progression of cell therapies to the clinic. The nacre surface from Pinctada maxima shells is known to enhance bone formation. However, to date, there is a paucity of information on the role of the topography of P. maxima surfaces, nacre and prism. To investigate this, nacre and prism topographical features were replicated onto polycaprolactone and skeletal stem cell behaviour on the surfaces studied. Skeletal stem cells on nacre surfaces exhibited an increase in cell area, increase in expression of osteogenic markers ALP (p < 0.05) and OCN (p < 0.01) and increased metabolite intensity (p < 0.05), indicating a role of nacre surface to induce osteogenic differentiation, while on prism surfaces, skeletal stem cells did not show alterations in cell area or osteogenic marker expression and a decrease in metabolite intensity (p < 0.05), demonstrating a distinct role for the prism surface, with the potential to maintain the skeletal stem cell phenotype. SAGE Publications 2018-09-04 /pmc/articles/PMC6124183/ /pubmed/30202512 http://dx.doi.org/10.1177/2041731418794007 Text en © The Author(s) 2018 http://www.creativecommons.org/licenses/by-nc/4.0/ This article is distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 License (http://www.creativecommons.org/licenses/by-nc/4.0/) which permits non-commercial use, reproduction and distribution of the work without further permission provided the original work is attributed as specified on the SAGE and Open Access pages (https://us.sagepub.com/en-us/nam/open-access-at-sage).
spellingShingle Cell Engineering at the Micro/Nanoscale – dedicated to Prof Adam Curtis
Waddell, Shona J
de Andrés, María C
Tsimbouri, Penelope M
Alakpa, Enateri V
Cusack, Maggie
Dalby, Matthew J
Oreffo, Richard OC
Biomimetic oyster shell–replicated topography alters the behaviour of human skeletal stem cells
title Biomimetic oyster shell–replicated topography alters the behaviour of human skeletal stem cells
title_full Biomimetic oyster shell–replicated topography alters the behaviour of human skeletal stem cells
title_fullStr Biomimetic oyster shell–replicated topography alters the behaviour of human skeletal stem cells
title_full_unstemmed Biomimetic oyster shell–replicated topography alters the behaviour of human skeletal stem cells
title_short Biomimetic oyster shell–replicated topography alters the behaviour of human skeletal stem cells
title_sort biomimetic oyster shell–replicated topography alters the behaviour of human skeletal stem cells
topic Cell Engineering at the Micro/Nanoscale – dedicated to Prof Adam Curtis
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6124183/
https://www.ncbi.nlm.nih.gov/pubmed/30202512
http://dx.doi.org/10.1177/2041731418794007
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