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Molecular Identification of Leishmania Parasites in Sand Flies (Diptera, Psychodidae) of an Endemic Foci in Poldokhtar, Iran

BACKGROUND: Cutaneous leishmaniasis is one of the most important public health problems in many developing countries. Sand flies, as vectors, transmit infectious forms of the parasite to the vertebrate hosts. Poldokhtar, South West of Iran, is one of the endemic foci of diseases with a little inform...

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Autores principales: Zivdari, Masoomeh, Hejazi, Seyed Hossein, Mirhendi, Seyed Hossein, Jafari, Reza, Rastegar, Hassan-Ali, Abtahi, Seyed Mohammad
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Medknow Publications & Media Pvt Ltd 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6124214/
https://www.ncbi.nlm.nih.gov/pubmed/30211137
http://dx.doi.org/10.4103/abr.abr_207_17
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author Zivdari, Masoomeh
Hejazi, Seyed Hossein
Mirhendi, Seyed Hossein
Jafari, Reza
Rastegar, Hassan-Ali
Abtahi, Seyed Mohammad
author_facet Zivdari, Masoomeh
Hejazi, Seyed Hossein
Mirhendi, Seyed Hossein
Jafari, Reza
Rastegar, Hassan-Ali
Abtahi, Seyed Mohammad
author_sort Zivdari, Masoomeh
collection PubMed
description BACKGROUND: Cutaneous leishmaniasis is one of the most important public health problems in many developing countries. Sand flies, as vectors, transmit infectious forms of the parasite to the vertebrate hosts. Poldokhtar, South West of Iran, is one of the endemic foci of diseases with a little information about it. In this paper, we have tried to gather some useful information to control and to prevent this disease in this region. MATERIALS AND METHODS: The present study was conducted to determine the vector(s), the parasite, and the species composition of sand flies in the Poldokhtar County during the months from July to September 2015. Sticky paper traps were used to collect sand flies from July to September. Species identification was done based on available diagnostic keys. Nested-polymerase chain reaction was performed to diagnosis the Leishmania infection of sand flies, and restriction fragment length polymorphism was used to identify the Leishmania species. RESULTS: A total of 2000 specimens comprising 8 species of sand flies (6 Phlebotomus and 2 Sergentomyia) were identified. Phlebotomus papatasi was the dominant species outdoor and Sergentomyia sintoni was the dominant species indoor. Among the 163 specimens of female P. papatasi, just 10 of them (6.1%) were positive to Leishmania major parasites. CONCLUSION: This is the first report of Leishmania infection of P. papatasi to L. major in this region. The results revealed that the high density of P. papatasi in outdoor and their infection with L. major is attributed that this species can play a major role as a principle vector in this region.
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spelling pubmed-61242142018-09-12 Molecular Identification of Leishmania Parasites in Sand Flies (Diptera, Psychodidae) of an Endemic Foci in Poldokhtar, Iran Zivdari, Masoomeh Hejazi, Seyed Hossein Mirhendi, Seyed Hossein Jafari, Reza Rastegar, Hassan-Ali Abtahi, Seyed Mohammad Adv Biomed Res Original Article BACKGROUND: Cutaneous leishmaniasis is one of the most important public health problems in many developing countries. Sand flies, as vectors, transmit infectious forms of the parasite to the vertebrate hosts. Poldokhtar, South West of Iran, is one of the endemic foci of diseases with a little information about it. In this paper, we have tried to gather some useful information to control and to prevent this disease in this region. MATERIALS AND METHODS: The present study was conducted to determine the vector(s), the parasite, and the species composition of sand flies in the Poldokhtar County during the months from July to September 2015. Sticky paper traps were used to collect sand flies from July to September. Species identification was done based on available diagnostic keys. Nested-polymerase chain reaction was performed to diagnosis the Leishmania infection of sand flies, and restriction fragment length polymorphism was used to identify the Leishmania species. RESULTS: A total of 2000 specimens comprising 8 species of sand flies (6 Phlebotomus and 2 Sergentomyia) were identified. Phlebotomus papatasi was the dominant species outdoor and Sergentomyia sintoni was the dominant species indoor. Among the 163 specimens of female P. papatasi, just 10 of them (6.1%) were positive to Leishmania major parasites. CONCLUSION: This is the first report of Leishmania infection of P. papatasi to L. major in this region. The results revealed that the high density of P. papatasi in outdoor and their infection with L. major is attributed that this species can play a major role as a principle vector in this region. Medknow Publications & Media Pvt Ltd 2018-08-31 /pmc/articles/PMC6124214/ /pubmed/30211137 http://dx.doi.org/10.4103/abr.abr_207_17 Text en Copyright: © 2018 Advanced Biomedical Research http://creativecommons.org/licenses/by-nc-sa/4.0 This is an open access journal, and articles are distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike 4.0 License, which allows others to remix, tweak, and build upon the work non-commercially, as long as appropriate credit is given and the new creations are licensed under the identical terms.
spellingShingle Original Article
Zivdari, Masoomeh
Hejazi, Seyed Hossein
Mirhendi, Seyed Hossein
Jafari, Reza
Rastegar, Hassan-Ali
Abtahi, Seyed Mohammad
Molecular Identification of Leishmania Parasites in Sand Flies (Diptera, Psychodidae) of an Endemic Foci in Poldokhtar, Iran
title Molecular Identification of Leishmania Parasites in Sand Flies (Diptera, Psychodidae) of an Endemic Foci in Poldokhtar, Iran
title_full Molecular Identification of Leishmania Parasites in Sand Flies (Diptera, Psychodidae) of an Endemic Foci in Poldokhtar, Iran
title_fullStr Molecular Identification of Leishmania Parasites in Sand Flies (Diptera, Psychodidae) of an Endemic Foci in Poldokhtar, Iran
title_full_unstemmed Molecular Identification of Leishmania Parasites in Sand Flies (Diptera, Psychodidae) of an Endemic Foci in Poldokhtar, Iran
title_short Molecular Identification of Leishmania Parasites in Sand Flies (Diptera, Psychodidae) of an Endemic Foci in Poldokhtar, Iran
title_sort molecular identification of leishmania parasites in sand flies (diptera, psychodidae) of an endemic foci in poldokhtar, iran
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6124214/
https://www.ncbi.nlm.nih.gov/pubmed/30211137
http://dx.doi.org/10.4103/abr.abr_207_17
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