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Deciphering the Contributions of CRH Receptors in the Brain and Pituitary to Stress-Induced Inhibition of the Reproductive Axis

Based on pharmacological studies, corticotropin-releasing hormone (CRH) and its receptors play a leading role in the inhibition of the hypothalamic–pituitary–gonadal (HPG) axis during acute stress. To further study the effects of CRH receptor signaling on the HPG axis, we generated and/or employed m...

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Autores principales: Raftogianni, Androniki, Roth, Lena C., García-González, Diego, Bus, Thorsten, Kühne, Claudia, Monyer, Hannah, Spergel, Daniel J., Deussing, Jan M., Grinevich, Valery
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6125327/
https://www.ncbi.nlm.nih.gov/pubmed/30214395
http://dx.doi.org/10.3389/fnmol.2018.00305
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author Raftogianni, Androniki
Roth, Lena C.
García-González, Diego
Bus, Thorsten
Kühne, Claudia
Monyer, Hannah
Spergel, Daniel J.
Deussing, Jan M.
Grinevich, Valery
author_facet Raftogianni, Androniki
Roth, Lena C.
García-González, Diego
Bus, Thorsten
Kühne, Claudia
Monyer, Hannah
Spergel, Daniel J.
Deussing, Jan M.
Grinevich, Valery
author_sort Raftogianni, Androniki
collection PubMed
description Based on pharmacological studies, corticotropin-releasing hormone (CRH) and its receptors play a leading role in the inhibition of the hypothalamic–pituitary–gonadal (HPG) axis during acute stress. To further study the effects of CRH receptor signaling on the HPG axis, we generated and/or employed male mice lacking CRH receptor type 1 (CRHR1) or type 2 (CRHR2) in gonadotropin-releasing hormone neurons, GABAergic neurons, or in all central neurons and glia. The deletion of CRHRs revealed a preserved decrease of plasma luteinizing hormone (LH) in response to either psychophysical or immunological stress. However, under basal conditions, central infusion of CRH into mice lacking CRHR1 in all central neurons and glia, or application of CRH to pituitary cultures from mice lacking CRHR2, failed to suppress LH release, unlike in controls. Our results, taken together with those of the earlier pharmacological studies, suggest that inhibition of the male HPG axis during acute stress is mediated by other factors along with CRH, and that CRH suppresses the HPG axis at the central and pituitary levels via CRHR1 and CRHR2, respectively.
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spelling pubmed-61253272018-09-13 Deciphering the Contributions of CRH Receptors in the Brain and Pituitary to Stress-Induced Inhibition of the Reproductive Axis Raftogianni, Androniki Roth, Lena C. García-González, Diego Bus, Thorsten Kühne, Claudia Monyer, Hannah Spergel, Daniel J. Deussing, Jan M. Grinevich, Valery Front Mol Neurosci Neuroscience Based on pharmacological studies, corticotropin-releasing hormone (CRH) and its receptors play a leading role in the inhibition of the hypothalamic–pituitary–gonadal (HPG) axis during acute stress. To further study the effects of CRH receptor signaling on the HPG axis, we generated and/or employed male mice lacking CRH receptor type 1 (CRHR1) or type 2 (CRHR2) in gonadotropin-releasing hormone neurons, GABAergic neurons, or in all central neurons and glia. The deletion of CRHRs revealed a preserved decrease of plasma luteinizing hormone (LH) in response to either psychophysical or immunological stress. However, under basal conditions, central infusion of CRH into mice lacking CRHR1 in all central neurons and glia, or application of CRH to pituitary cultures from mice lacking CRHR2, failed to suppress LH release, unlike in controls. Our results, taken together with those of the earlier pharmacological studies, suggest that inhibition of the male HPG axis during acute stress is mediated by other factors along with CRH, and that CRH suppresses the HPG axis at the central and pituitary levels via CRHR1 and CRHR2, respectively. Frontiers Media S.A. 2018-08-30 /pmc/articles/PMC6125327/ /pubmed/30214395 http://dx.doi.org/10.3389/fnmol.2018.00305 Text en Copyright © 2018 Raftogianni, Roth, García-González, Bus, Kühne, Monyer, Spergel, Deussing and Grinevich. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Neuroscience
Raftogianni, Androniki
Roth, Lena C.
García-González, Diego
Bus, Thorsten
Kühne, Claudia
Monyer, Hannah
Spergel, Daniel J.
Deussing, Jan M.
Grinevich, Valery
Deciphering the Contributions of CRH Receptors in the Brain and Pituitary to Stress-Induced Inhibition of the Reproductive Axis
title Deciphering the Contributions of CRH Receptors in the Brain and Pituitary to Stress-Induced Inhibition of the Reproductive Axis
title_full Deciphering the Contributions of CRH Receptors in the Brain and Pituitary to Stress-Induced Inhibition of the Reproductive Axis
title_fullStr Deciphering the Contributions of CRH Receptors in the Brain and Pituitary to Stress-Induced Inhibition of the Reproductive Axis
title_full_unstemmed Deciphering the Contributions of CRH Receptors in the Brain and Pituitary to Stress-Induced Inhibition of the Reproductive Axis
title_short Deciphering the Contributions of CRH Receptors in the Brain and Pituitary to Stress-Induced Inhibition of the Reproductive Axis
title_sort deciphering the contributions of crh receptors in the brain and pituitary to stress-induced inhibition of the reproductive axis
topic Neuroscience
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6125327/
https://www.ncbi.nlm.nih.gov/pubmed/30214395
http://dx.doi.org/10.3389/fnmol.2018.00305
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