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A WUSCHEL Homeobox Transcription Factor, OsWOX13, Enhances Drought Tolerance and Triggers Early Flowering in Rice

Plants have evolved strategies to cope with drought stress by maximizing physiological capacity and adjusting developmental processes such as flowering time. The WOX13 orthologous group is the most conserved among the clade of WOX homeodomain-containing proteins and is found to function in both drou...

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Autores principales: Minh-Thu, Pham-Thi, Kim, Joung Sug, Chae, Songhwa, Jun, Kyong Mi, Lee, Gang-Seob, Kim, Dong-Eun, Cheong, Jong-Joo, Song, Sang Ik, Nahm, Baek Hie, Kim, Yeon-Ki
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Korean Society for Molecular and Cellular Biology 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6125423/
https://www.ncbi.nlm.nih.gov/pubmed/30078233
http://dx.doi.org/10.14348/molcells.2018.0203
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author Minh-Thu, Pham-Thi
Kim, Joung Sug
Chae, Songhwa
Jun, Kyong Mi
Lee, Gang-Seob
Kim, Dong-Eun
Cheong, Jong-Joo
Song, Sang Ik
Nahm, Baek Hie
Kim, Yeon-Ki
author_facet Minh-Thu, Pham-Thi
Kim, Joung Sug
Chae, Songhwa
Jun, Kyong Mi
Lee, Gang-Seob
Kim, Dong-Eun
Cheong, Jong-Joo
Song, Sang Ik
Nahm, Baek Hie
Kim, Yeon-Ki
author_sort Minh-Thu, Pham-Thi
collection PubMed
description Plants have evolved strategies to cope with drought stress by maximizing physiological capacity and adjusting developmental processes such as flowering time. The WOX13 orthologous group is the most conserved among the clade of WOX homeodomain-containing proteins and is found to function in both drought stress and flower development. In this study, we isolated and characterized OsWOX13 from rice. OsWOX13 was regulated spatially in vegetative organs but temporally in flowers and seeds. Overexpression of OsWOX13 (OsWOX13-ov) in rice under the rab21 promoter resulted in drought resistance and early flowering by 7–10 days. Screening of gene expression profiles in mature leaf and panicles of OsWOX13-ov showed a broad spectrum of effects on biological processes, such as abiotic and biotic stresses, exerting a cross-talk between responses. Protein binding microarray and electrophoretic mobility shift assay analyses supported ATTGATTG as the putative cis-element binding of OsWOX13. OsDREB1A and OsDREB1F, drought stress response transcription factors, contain ATTGATTG motif(s) in their promoters and are preferentially expressed in OsWOX13-ov. In addition, Heading date 3a and OsMADS14, regulators in the flowering pathway and development, were enhanced in OsWOX13-ov. These results suggest that OsWOX13 mediates the stress response and early flowering and, thus, may be a regulator of genes involved in drought escape.
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spelling pubmed-61254232018-09-10 A WUSCHEL Homeobox Transcription Factor, OsWOX13, Enhances Drought Tolerance and Triggers Early Flowering in Rice Minh-Thu, Pham-Thi Kim, Joung Sug Chae, Songhwa Jun, Kyong Mi Lee, Gang-Seob Kim, Dong-Eun Cheong, Jong-Joo Song, Sang Ik Nahm, Baek Hie Kim, Yeon-Ki Mol Cells Article Plants have evolved strategies to cope with drought stress by maximizing physiological capacity and adjusting developmental processes such as flowering time. The WOX13 orthologous group is the most conserved among the clade of WOX homeodomain-containing proteins and is found to function in both drought stress and flower development. In this study, we isolated and characterized OsWOX13 from rice. OsWOX13 was regulated spatially in vegetative organs but temporally in flowers and seeds. Overexpression of OsWOX13 (OsWOX13-ov) in rice under the rab21 promoter resulted in drought resistance and early flowering by 7–10 days. Screening of gene expression profiles in mature leaf and panicles of OsWOX13-ov showed a broad spectrum of effects on biological processes, such as abiotic and biotic stresses, exerting a cross-talk between responses. Protein binding microarray and electrophoretic mobility shift assay analyses supported ATTGATTG as the putative cis-element binding of OsWOX13. OsDREB1A and OsDREB1F, drought stress response transcription factors, contain ATTGATTG motif(s) in their promoters and are preferentially expressed in OsWOX13-ov. In addition, Heading date 3a and OsMADS14, regulators in the flowering pathway and development, were enhanced in OsWOX13-ov. These results suggest that OsWOX13 mediates the stress response and early flowering and, thus, may be a regulator of genes involved in drought escape. Korean Society for Molecular and Cellular Biology 2018-08-31 2018-07-30 /pmc/articles/PMC6125423/ /pubmed/30078233 http://dx.doi.org/10.14348/molcells.2018.0203 Text en © The Korean Society for Molecular and Cellular Biology. All rights reserved. This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike 3.0 Unported License. To view a copy of this license, visit http://creativecommons.org/licenses/by-nc-sa/3.0/.
spellingShingle Article
Minh-Thu, Pham-Thi
Kim, Joung Sug
Chae, Songhwa
Jun, Kyong Mi
Lee, Gang-Seob
Kim, Dong-Eun
Cheong, Jong-Joo
Song, Sang Ik
Nahm, Baek Hie
Kim, Yeon-Ki
A WUSCHEL Homeobox Transcription Factor, OsWOX13, Enhances Drought Tolerance and Triggers Early Flowering in Rice
title A WUSCHEL Homeobox Transcription Factor, OsWOX13, Enhances Drought Tolerance and Triggers Early Flowering in Rice
title_full A WUSCHEL Homeobox Transcription Factor, OsWOX13, Enhances Drought Tolerance and Triggers Early Flowering in Rice
title_fullStr A WUSCHEL Homeobox Transcription Factor, OsWOX13, Enhances Drought Tolerance and Triggers Early Flowering in Rice
title_full_unstemmed A WUSCHEL Homeobox Transcription Factor, OsWOX13, Enhances Drought Tolerance and Triggers Early Flowering in Rice
title_short A WUSCHEL Homeobox Transcription Factor, OsWOX13, Enhances Drought Tolerance and Triggers Early Flowering in Rice
title_sort wuschel homeobox transcription factor, oswox13, enhances drought tolerance and triggers early flowering in rice
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6125423/
https://www.ncbi.nlm.nih.gov/pubmed/30078233
http://dx.doi.org/10.14348/molcells.2018.0203
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