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Preparation and characterization of manganese, cobalt and zinc DNA nanoflowers with tuneable morphology, DNA content and size

Recently reported DNA nanoflowers are an interesting class of organic-inorganic hybrid materials which are prepared using DNA polymerases. DNA nanoflowers combine the high surface area and scaffolding of inorganic Mg(2)P(2)O(7) nanocrystals with the targeting properties of DNA, whilst adding enzymat...

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Autores principales: Baker, Ysobel R, Chen, Jinfeng, Brown, Jason, El-Sagheer, Afaf H, Wiseman, Philip, Johnson, Errin, Goddard, Paul, Brown, Tom
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6125639/
https://www.ncbi.nlm.nih.gov/pubmed/30010979
http://dx.doi.org/10.1093/nar/gky630
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author Baker, Ysobel R
Chen, Jinfeng
Brown, Jason
El-Sagheer, Afaf H
Wiseman, Philip
Johnson, Errin
Goddard, Paul
Brown, Tom
author_facet Baker, Ysobel R
Chen, Jinfeng
Brown, Jason
El-Sagheer, Afaf H
Wiseman, Philip
Johnson, Errin
Goddard, Paul
Brown, Tom
author_sort Baker, Ysobel R
collection PubMed
description Recently reported DNA nanoflowers are an interesting class of organic-inorganic hybrid materials which are prepared using DNA polymerases. DNA nanoflowers combine the high surface area and scaffolding of inorganic Mg(2)P(2)O(7) nanocrystals with the targeting properties of DNA, whilst adding enzymatic stability and enhanced cellular uptake. We have investigated conditions for chemically modifying the inorganic core of these nanoflowers through substitution of Mg(2+) with Mn(2+), Co(2+) or Zn(2+) and have characterized the resulting particles. These have a range of novel nanoarchitectures, retain the enzymatic stability of their magnesium counterparts and the Co(2+) and Mn(2+) DNA nanoflowers have added magnetic properties. We investigate conditions to control different morphologies, DNA content, hybridization properties, and size. Additionally, we show that DNA nanoflower production is not limited to Ф29 DNA polymerase and that the choice of polymerase can influence the DNA length within the constructs. We anticipate that the added control of structure, size and chemistry will enhance future applications.
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spelling pubmed-61256392018-09-11 Preparation and characterization of manganese, cobalt and zinc DNA nanoflowers with tuneable morphology, DNA content and size Baker, Ysobel R Chen, Jinfeng Brown, Jason El-Sagheer, Afaf H Wiseman, Philip Johnson, Errin Goddard, Paul Brown, Tom Nucleic Acids Res Chemical Biology and Nucleic Acid Chemistry Recently reported DNA nanoflowers are an interesting class of organic-inorganic hybrid materials which are prepared using DNA polymerases. DNA nanoflowers combine the high surface area and scaffolding of inorganic Mg(2)P(2)O(7) nanocrystals with the targeting properties of DNA, whilst adding enzymatic stability and enhanced cellular uptake. We have investigated conditions for chemically modifying the inorganic core of these nanoflowers through substitution of Mg(2+) with Mn(2+), Co(2+) or Zn(2+) and have characterized the resulting particles. These have a range of novel nanoarchitectures, retain the enzymatic stability of their magnesium counterparts and the Co(2+) and Mn(2+) DNA nanoflowers have added magnetic properties. We investigate conditions to control different morphologies, DNA content, hybridization properties, and size. Additionally, we show that DNA nanoflower production is not limited to Ф29 DNA polymerase and that the choice of polymerase can influence the DNA length within the constructs. We anticipate that the added control of structure, size and chemistry will enhance future applications. Oxford University Press 2018-09-06 2018-07-12 /pmc/articles/PMC6125639/ /pubmed/30010979 http://dx.doi.org/10.1093/nar/gky630 Text en © The Author(s) 2018. Published by Oxford University Press on behalf of Nucleic Acids Research. http://creativecommons.org/licenses/by/4.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Chemical Biology and Nucleic Acid Chemistry
Baker, Ysobel R
Chen, Jinfeng
Brown, Jason
El-Sagheer, Afaf H
Wiseman, Philip
Johnson, Errin
Goddard, Paul
Brown, Tom
Preparation and characterization of manganese, cobalt and zinc DNA nanoflowers with tuneable morphology, DNA content and size
title Preparation and characterization of manganese, cobalt and zinc DNA nanoflowers with tuneable morphology, DNA content and size
title_full Preparation and characterization of manganese, cobalt and zinc DNA nanoflowers with tuneable morphology, DNA content and size
title_fullStr Preparation and characterization of manganese, cobalt and zinc DNA nanoflowers with tuneable morphology, DNA content and size
title_full_unstemmed Preparation and characterization of manganese, cobalt and zinc DNA nanoflowers with tuneable morphology, DNA content and size
title_short Preparation and characterization of manganese, cobalt and zinc DNA nanoflowers with tuneable morphology, DNA content and size
title_sort preparation and characterization of manganese, cobalt and zinc dna nanoflowers with tuneable morphology, dna content and size
topic Chemical Biology and Nucleic Acid Chemistry
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6125639/
https://www.ncbi.nlm.nih.gov/pubmed/30010979
http://dx.doi.org/10.1093/nar/gky630
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