Cargando…

MicroRNA-124 inhibits cell proliferation, invasion and migration by targeting CAV1 in bladder cancer

MicroRNAs (miRs) may have promotive or suppressive roles in various human cancers types, but the molecular mechanisms underlying the role of miR-124 in bladder cancer (BC) progression have remained largely elusive. In the present study, it was observed that miR-124 was significantly downregulated in...

Descripción completa

Detalles Bibliográficos
Autores principales: Zhou, Wandan, He, Leye, Dai, Yinbo, Zhang, Yichuan, Wang, Jinrong, Liu, Bin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: D.A. Spandidos 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6125959/
https://www.ncbi.nlm.nih.gov/pubmed/30214503
http://dx.doi.org/10.3892/etm.2018.6537
_version_ 1783353247276204032
author Zhou, Wandan
He, Leye
Dai, Yinbo
Zhang, Yichuan
Wang, Jinrong
Liu, Bin
author_facet Zhou, Wandan
He, Leye
Dai, Yinbo
Zhang, Yichuan
Wang, Jinrong
Liu, Bin
author_sort Zhou, Wandan
collection PubMed
description MicroRNAs (miRs) may have promotive or suppressive roles in various human cancers types, but the molecular mechanisms underlying the role of miR-124 in bladder cancer (BC) progression have remained largely elusive. In the present study, it was observed that miR-124 was significantly downregulated in BC tissues compared with that in adjacent non-neoplastic tissues. Furthermore, its expression was also reduced in several human BC cell lines (T24, HT-1376 and 5637) compared with that in the normal bladder epithelial SV-HUC-1 cell line. A low expression of miR-124 in BC patients was significantly associated with advanced malignancy and a poor prognosis. Caveolin 1 (CAV1) was identified as a novel target gene of miR-124, and the expression of CAV1 was negatively regulated by miR-124 in T24 cells. Furthermore, CAV1 was identified to be significantly upregulated in BC tissues and cell lines, and a negative correlation was observed between the expression of miR-124 and CAV1 in BC tissues. Furthermore, restoration of miR-124 expression significantly inhibited the proliferation, migration and invasion of T24 cells, and these effects were impaired following overexpression of CAV1. Taken together, the present results demonstrate that miR-124 has a suppressive role in the proliferation, migration and invasion of BC cells by targeting CAV1, which suggests that miR-124 is a potential therapeutic candidate for BC.
format Online
Article
Text
id pubmed-6125959
institution National Center for Biotechnology Information
language English
publishDate 2018
publisher D.A. Spandidos
record_format MEDLINE/PubMed
spelling pubmed-61259592018-09-13 MicroRNA-124 inhibits cell proliferation, invasion and migration by targeting CAV1 in bladder cancer Zhou, Wandan He, Leye Dai, Yinbo Zhang, Yichuan Wang, Jinrong Liu, Bin Exp Ther Med Articles MicroRNAs (miRs) may have promotive or suppressive roles in various human cancers types, but the molecular mechanisms underlying the role of miR-124 in bladder cancer (BC) progression have remained largely elusive. In the present study, it was observed that miR-124 was significantly downregulated in BC tissues compared with that in adjacent non-neoplastic tissues. Furthermore, its expression was also reduced in several human BC cell lines (T24, HT-1376 and 5637) compared with that in the normal bladder epithelial SV-HUC-1 cell line. A low expression of miR-124 in BC patients was significantly associated with advanced malignancy and a poor prognosis. Caveolin 1 (CAV1) was identified as a novel target gene of miR-124, and the expression of CAV1 was negatively regulated by miR-124 in T24 cells. Furthermore, CAV1 was identified to be significantly upregulated in BC tissues and cell lines, and a negative correlation was observed between the expression of miR-124 and CAV1 in BC tissues. Furthermore, restoration of miR-124 expression significantly inhibited the proliferation, migration and invasion of T24 cells, and these effects were impaired following overexpression of CAV1. Taken together, the present results demonstrate that miR-124 has a suppressive role in the proliferation, migration and invasion of BC cells by targeting CAV1, which suggests that miR-124 is a potential therapeutic candidate for BC. D.A. Spandidos 2018-10 2018-07-30 /pmc/articles/PMC6125959/ /pubmed/30214503 http://dx.doi.org/10.3892/etm.2018.6537 Text en Copyright: © Zhou et al. This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License (https://creativecommons.org/licenses/by-nc-nd/4.0/) , which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.
spellingShingle Articles
Zhou, Wandan
He, Leye
Dai, Yinbo
Zhang, Yichuan
Wang, Jinrong
Liu, Bin
MicroRNA-124 inhibits cell proliferation, invasion and migration by targeting CAV1 in bladder cancer
title MicroRNA-124 inhibits cell proliferation, invasion and migration by targeting CAV1 in bladder cancer
title_full MicroRNA-124 inhibits cell proliferation, invasion and migration by targeting CAV1 in bladder cancer
title_fullStr MicroRNA-124 inhibits cell proliferation, invasion and migration by targeting CAV1 in bladder cancer
title_full_unstemmed MicroRNA-124 inhibits cell proliferation, invasion and migration by targeting CAV1 in bladder cancer
title_short MicroRNA-124 inhibits cell proliferation, invasion and migration by targeting CAV1 in bladder cancer
title_sort microrna-124 inhibits cell proliferation, invasion and migration by targeting cav1 in bladder cancer
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6125959/
https://www.ncbi.nlm.nih.gov/pubmed/30214503
http://dx.doi.org/10.3892/etm.2018.6537
work_keys_str_mv AT zhouwandan microrna124inhibitscellproliferationinvasionandmigrationbytargetingcav1inbladdercancer
AT heleye microrna124inhibitscellproliferationinvasionandmigrationbytargetingcav1inbladdercancer
AT daiyinbo microrna124inhibitscellproliferationinvasionandmigrationbytargetingcav1inbladdercancer
AT zhangyichuan microrna124inhibitscellproliferationinvasionandmigrationbytargetingcav1inbladdercancer
AT wangjinrong microrna124inhibitscellproliferationinvasionandmigrationbytargetingcav1inbladdercancer
AT liubin microrna124inhibitscellproliferationinvasionandmigrationbytargetingcav1inbladdercancer