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Preparation of Functional Silica Using a Bioinspired Method
The goal of the protocols described herein is to synthesize bioinspired silica materials, perform enzyme encapsulation therein, and partially or totally purify the same by acid elution. By combining sodium silicate with a polyfunctional bioinspired additive, silica is rapidly formed at ambient condi...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MyJove Corporation
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6126601/ https://www.ncbi.nlm.nih.gov/pubmed/30124655 http://dx.doi.org/10.3791/57730 |
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author | Manning, Joseph R.H. Routoula, Eleni Patwardhan, Siddharth V. |
author_facet | Manning, Joseph R.H. Routoula, Eleni Patwardhan, Siddharth V. |
author_sort | Manning, Joseph R.H. |
collection | PubMed |
description | The goal of the protocols described herein is to synthesize bioinspired silica materials, perform enzyme encapsulation therein, and partially or totally purify the same by acid elution. By combining sodium silicate with a polyfunctional bioinspired additive, silica is rapidly formed at ambient conditions upon neutralization. The effect of neutralization rate and biomolecule addition point on silica yield are investigated, and biomolecule immobilization efficiency is reported for varying addition point. In contrast to other porous silica synthesis methods, it is shown that the mild conditions required for bioinspired silica synthesis are fully compatible with the encapsulation of delicate biomolecules. Additionally, mild conditions are used across all synthesis and modification steps, making bioinspired silica a promising target for the scale-up and commercialization as both a bare material and active support medium. The synthesis is shown to be highly sensitive to conditions, i.e., the neutralization rate and final synthesis pH, however tight control over these parameters is demonstrated through the use of auto titration methods, leading to high reproducibility in reaction progression pathway and yield. Therefore, bioinspired silica is an excellent active material support choice, showing versatility towards many current applications, not limited to those demonstrated here, and potency in future applications. |
format | Online Article Text |
id | pubmed-6126601 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | MyJove Corporation |
record_format | MEDLINE/PubMed |
spelling | pubmed-61266012018-09-19 Preparation of Functional Silica Using a Bioinspired Method Manning, Joseph R.H. Routoula, Eleni Patwardhan, Siddharth V. J Vis Exp Chemistry The goal of the protocols described herein is to synthesize bioinspired silica materials, perform enzyme encapsulation therein, and partially or totally purify the same by acid elution. By combining sodium silicate with a polyfunctional bioinspired additive, silica is rapidly formed at ambient conditions upon neutralization. The effect of neutralization rate and biomolecule addition point on silica yield are investigated, and biomolecule immobilization efficiency is reported for varying addition point. In contrast to other porous silica synthesis methods, it is shown that the mild conditions required for bioinspired silica synthesis are fully compatible with the encapsulation of delicate biomolecules. Additionally, mild conditions are used across all synthesis and modification steps, making bioinspired silica a promising target for the scale-up and commercialization as both a bare material and active support medium. The synthesis is shown to be highly sensitive to conditions, i.e., the neutralization rate and final synthesis pH, however tight control over these parameters is demonstrated through the use of auto titration methods, leading to high reproducibility in reaction progression pathway and yield. Therefore, bioinspired silica is an excellent active material support choice, showing versatility towards many current applications, not limited to those demonstrated here, and potency in future applications. MyJove Corporation 2018-08-01 /pmc/articles/PMC6126601/ /pubmed/30124655 http://dx.doi.org/10.3791/57730 Text en Copyright © 2018, Journal of Visualized Experiments http://creativecommons.org/licenses/by/3.0/us/ This is an open-access article distributed under the terms of the Creative Commons Attribution 3.0 License. To view a copy of this license, visithttp://creativecommons.org/licenses/by/3.0/us/ |
spellingShingle | Chemistry Manning, Joseph R.H. Routoula, Eleni Patwardhan, Siddharth V. Preparation of Functional Silica Using a Bioinspired Method |
title | Preparation of Functional Silica Using a Bioinspired Method |
title_full | Preparation of Functional Silica Using a Bioinspired Method |
title_fullStr | Preparation of Functional Silica Using a Bioinspired Method |
title_full_unstemmed | Preparation of Functional Silica Using a Bioinspired Method |
title_short | Preparation of Functional Silica Using a Bioinspired Method |
title_sort | preparation of functional silica using a bioinspired method |
topic | Chemistry |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6126601/ https://www.ncbi.nlm.nih.gov/pubmed/30124655 http://dx.doi.org/10.3791/57730 |
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