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Theileria parva antigens recognized by CD8+ T cells show varying degrees of diversity in buffalo-derived infected cell lines

The extent of sequence diversity among the genes encoding 10 antigens (Tp1–10) known to be recognized by CD8(+) T lymphocytes from cattle immune to Theileria parva was analysed. The sequences were derived from parasites in 23 buffalo-derived cell lines, three cattle-derived isolates and one cloned c...

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Autores principales: Sitt, Tatjana, Pelle, Roger, Chepkwony, Maurine, Morrison, W. Ivan, Toye, Philip
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Cambridge University Press 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6126638/
https://www.ncbi.nlm.nih.gov/pubmed/29729680
http://dx.doi.org/10.1017/S0031182018000264
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author Sitt, Tatjana
Pelle, Roger
Chepkwony, Maurine
Morrison, W. Ivan
Toye, Philip
author_facet Sitt, Tatjana
Pelle, Roger
Chepkwony, Maurine
Morrison, W. Ivan
Toye, Philip
author_sort Sitt, Tatjana
collection PubMed
description The extent of sequence diversity among the genes encoding 10 antigens (Tp1–10) known to be recognized by CD8(+) T lymphocytes from cattle immune to Theileria parva was analysed. The sequences were derived from parasites in 23 buffalo-derived cell lines, three cattle-derived isolates and one cloned cell line obtained from a buffalo-derived stabilate. The results revealed substantial variation among the antigens through sequence diversity. The greatest nucleotide and amino acid diversity were observed in Tp1, Tp2 and Tp9. Tp5 and Tp7 showed the least amount of allelic diversity, and Tp5, Tp6 and Tp7 had the lowest levels of protein diversity. Tp6 was the most conserved protein; only a single non-synonymous substitution was found in all obtained sequences. The ratio of non-synonymous: synonymous substitutions varied from 0.84 (Tp1) to 0.04 (Tp6). Apart from Tp2 and Tp9, we observed no variation in the other defined CD8+ T cell epitopes (Tp4, 5, 7 and 8), indicating that epitope variation is not a universal feature of T. parva antigens. In addition to providing markers that can be used to examine the diversity in T. parva populations, the results highlight the potential for using conserved antigens to develop vaccines that provide broad protection against T. parva.
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spelling pubmed-61266382018-09-17 Theileria parva antigens recognized by CD8+ T cells show varying degrees of diversity in buffalo-derived infected cell lines Sitt, Tatjana Pelle, Roger Chepkwony, Maurine Morrison, W. Ivan Toye, Philip Parasitology Research Article The extent of sequence diversity among the genes encoding 10 antigens (Tp1–10) known to be recognized by CD8(+) T lymphocytes from cattle immune to Theileria parva was analysed. The sequences were derived from parasites in 23 buffalo-derived cell lines, three cattle-derived isolates and one cloned cell line obtained from a buffalo-derived stabilate. The results revealed substantial variation among the antigens through sequence diversity. The greatest nucleotide and amino acid diversity were observed in Tp1, Tp2 and Tp9. Tp5 and Tp7 showed the least amount of allelic diversity, and Tp5, Tp6 and Tp7 had the lowest levels of protein diversity. Tp6 was the most conserved protein; only a single non-synonymous substitution was found in all obtained sequences. The ratio of non-synonymous: synonymous substitutions varied from 0.84 (Tp1) to 0.04 (Tp6). Apart from Tp2 and Tp9, we observed no variation in the other defined CD8+ T cell epitopes (Tp4, 5, 7 and 8), indicating that epitope variation is not a universal feature of T. parva antigens. In addition to providing markers that can be used to examine the diversity in T. parva populations, the results highlight the potential for using conserved antigens to develop vaccines that provide broad protection against T. parva. Cambridge University Press 2018-09 2018-05-06 /pmc/articles/PMC6126638/ /pubmed/29729680 http://dx.doi.org/10.1017/S0031182018000264 Text en © Cambridge University Press 2018 http://creativecommons.org/licenses/by/4.0/ This is an Open Access article, distributed under the terms of the Creative Commons Attribution licence (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted re-use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Sitt, Tatjana
Pelle, Roger
Chepkwony, Maurine
Morrison, W. Ivan
Toye, Philip
Theileria parva antigens recognized by CD8+ T cells show varying degrees of diversity in buffalo-derived infected cell lines
title Theileria parva antigens recognized by CD8+ T cells show varying degrees of diversity in buffalo-derived infected cell lines
title_full Theileria parva antigens recognized by CD8+ T cells show varying degrees of diversity in buffalo-derived infected cell lines
title_fullStr Theileria parva antigens recognized by CD8+ T cells show varying degrees of diversity in buffalo-derived infected cell lines
title_full_unstemmed Theileria parva antigens recognized by CD8+ T cells show varying degrees of diversity in buffalo-derived infected cell lines
title_short Theileria parva antigens recognized by CD8+ T cells show varying degrees of diversity in buffalo-derived infected cell lines
title_sort theileria parva antigens recognized by cd8+ t cells show varying degrees of diversity in buffalo-derived infected cell lines
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6126638/
https://www.ncbi.nlm.nih.gov/pubmed/29729680
http://dx.doi.org/10.1017/S0031182018000264
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