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The Barnacle Balanus improvisus as a Marine Model - Culturing and Gene Expression

Barnacles are marine crustaceans with a sessile adult and free-swimming, planktonic larvae. The barnacle Balanus (Amphibalanus) improvisus is particularly relevant as a model for the studies of osmoregulatory mechanisms because of its extreme tolerance to low salinity. It is also widely used as a mo...

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Autores principales: Jonsson, Per R., Wrange, Anna-Lisa, Lind, Ulrika, Abramova, Anna, Ogemark, Martin, Blomberg, Anders
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MyJove Corporation 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6126684/
https://www.ncbi.nlm.nih.gov/pubmed/30148484
http://dx.doi.org/10.3791/57825
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author Jonsson, Per R.
Wrange, Anna-Lisa
Lind, Ulrika
Abramova, Anna
Ogemark, Martin
Blomberg, Anders
author_facet Jonsson, Per R.
Wrange, Anna-Lisa
Lind, Ulrika
Abramova, Anna
Ogemark, Martin
Blomberg, Anders
author_sort Jonsson, Per R.
collection PubMed
description Barnacles are marine crustaceans with a sessile adult and free-swimming, planktonic larvae. The barnacle Balanus (Amphibalanus) improvisus is particularly relevant as a model for the studies of osmoregulatory mechanisms because of its extreme tolerance to low salinity. It is also widely used as a model of settling biology, in particular in relation to antifouling research. However, natural seasonal spawning yields an unpredictable supply of cyprid larvae for studies. A protocol for the all-year-round culturing of B. improvisus has been developed and a detailed description of all steps in the production line is outlined (i.e., the establishment of adult cultures on panels, the collection and rearing of barnacle larvae, and the administration of feed for adults and larvae). The description also provides guidance on troubleshooting and discusses critical parameters (e.g., the removal of contamination, the production of high-quality feed, the manpower needed, and the importance of high-quality seawater). Each batch from the culturing system maximally yields roughly 12,000 nauplii and can deliver four batches in a week, so up to almost 50,000 larvae per week can be produced. The method used to culture B. improvisus is, probably, to a large extent also applicable to other marine invertebrates with free-swimminglarvae. Protocols are presented for the dissection of various tissues from adults as well as the production of high-quality RNA for studies on gene expression. It is also described how cultured adults and reared cyprids can be utilized in a wide array of experimental designs for examining gene expression in relation to external factors. The use of cultured barnacles in gene expression is illustrated with studies of possible osmoregulatory roles of Na(+)/K(+) ATPase and aquaporins.
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spelling pubmed-61266842018-09-19 The Barnacle Balanus improvisus as a Marine Model - Culturing and Gene Expression Jonsson, Per R. Wrange, Anna-Lisa Lind, Ulrika Abramova, Anna Ogemark, Martin Blomberg, Anders J Vis Exp Environmental Sciences Barnacles are marine crustaceans with a sessile adult and free-swimming, planktonic larvae. The barnacle Balanus (Amphibalanus) improvisus is particularly relevant as a model for the studies of osmoregulatory mechanisms because of its extreme tolerance to low salinity. It is also widely used as a model of settling biology, in particular in relation to antifouling research. However, natural seasonal spawning yields an unpredictable supply of cyprid larvae for studies. A protocol for the all-year-round culturing of B. improvisus has been developed and a detailed description of all steps in the production line is outlined (i.e., the establishment of adult cultures on panels, the collection and rearing of barnacle larvae, and the administration of feed for adults and larvae). The description also provides guidance on troubleshooting and discusses critical parameters (e.g., the removal of contamination, the production of high-quality feed, the manpower needed, and the importance of high-quality seawater). Each batch from the culturing system maximally yields roughly 12,000 nauplii and can deliver four batches in a week, so up to almost 50,000 larvae per week can be produced. The method used to culture B. improvisus is, probably, to a large extent also applicable to other marine invertebrates with free-swimminglarvae. Protocols are presented for the dissection of various tissues from adults as well as the production of high-quality RNA for studies on gene expression. It is also described how cultured adults and reared cyprids can be utilized in a wide array of experimental designs for examining gene expression in relation to external factors. The use of cultured barnacles in gene expression is illustrated with studies of possible osmoregulatory roles of Na(+)/K(+) ATPase and aquaporins. MyJove Corporation 2018-08-08 /pmc/articles/PMC6126684/ /pubmed/30148484 http://dx.doi.org/10.3791/57825 Text en Copyright © 2018, Journal of Visualized Experiments http://creativecommons.org/licenses/by-nc-nd/3.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs 3.0 Unported License. To view a copy of this license, visithttp://creativecommons.org/licenses/by-nc-nd/3.0/
spellingShingle Environmental Sciences
Jonsson, Per R.
Wrange, Anna-Lisa
Lind, Ulrika
Abramova, Anna
Ogemark, Martin
Blomberg, Anders
The Barnacle Balanus improvisus as a Marine Model - Culturing and Gene Expression
title The Barnacle Balanus improvisus as a Marine Model - Culturing and Gene Expression
title_full The Barnacle Balanus improvisus as a Marine Model - Culturing and Gene Expression
title_fullStr The Barnacle Balanus improvisus as a Marine Model - Culturing and Gene Expression
title_full_unstemmed The Barnacle Balanus improvisus as a Marine Model - Culturing and Gene Expression
title_short The Barnacle Balanus improvisus as a Marine Model - Culturing and Gene Expression
title_sort barnacle balanus improvisus as a marine model - culturing and gene expression
topic Environmental Sciences
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6126684/
https://www.ncbi.nlm.nih.gov/pubmed/30148484
http://dx.doi.org/10.3791/57825
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