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A tRNA-based multiplex sgRNA expression system in zebrafish and its application to generation of transgenic albino fish
The CRISPR/Cas9 system can be introduced into zebrafish as transgenes. Namely, expression of single-guide RNA (sgRNA) and controlled expression of Cas9 in transgenic zebrafish enables the study of gene functions in specific cell types. This transgenic CRISPR/Cas9 approach would be more useful if mul...
| Autores principales: | , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
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Nature Publishing Group UK
2018
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| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6127137/ https://www.ncbi.nlm.nih.gov/pubmed/30190522 http://dx.doi.org/10.1038/s41598-018-31476-5 |