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Chemical characterization and antioxidant properties of exopolysaccharides from mangrove filamentous fungi Fusarium equiseti ANP2
A filamentous fungi F. equiseti strain ANP2 with good exopolysaccharide (EPS) yield, apparent viscosity was screened and isolated from Krishna estuarine mangrove sediments. Based on molecular characteristics the selected strain was confirmed as F. equiseti, yielded EPS (4.9 ± 0.25 g/L) and whole cel...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6127374/ https://www.ncbi.nlm.nih.gov/pubmed/30197873 http://dx.doi.org/10.1016/j.btre.2018.e00277 |
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author | Prathyusha, A.M.V.N. Mohana Sheela, G. Bramhachari, P.V. |
author_facet | Prathyusha, A.M.V.N. Mohana Sheela, G. Bramhachari, P.V. |
author_sort | Prathyusha, A.M.V.N. |
collection | PubMed |
description | A filamentous fungi F. equiseti strain ANP2 with good exopolysaccharide (EPS) yield, apparent viscosity was screened and isolated from Krishna estuarine mangrove sediments. Based on molecular characteristics the selected strain was confirmed as F. equiseti, yielded EPS (4.9 ± 0.25 g/L) and whole cell biomass (3.5 ± 0.2 g/L) in MSM during early stationary phase. The crude EPS was purified by chromatography of Q-Sepharose and Superdex-75, affording Ms and MF-1 fractions were obtained, respectively. Chemical analyses revealed that EPS is primarily composed of neutral sugars and proteins. FTIR-spectroscopy revealed presence of hydroxyl, carbonyl groups and glycosidic bonds which correspond to typical heteropolysaccharide. GC analysis demonstrated that MF-1 mainly consist of mannose (72.6%) and glucose (27.3%). Viscosity studies exhibited typical non-Newtonian pseudoplastic behaviour with high viscosity. The scavenging ability on hydroxyl radicals, indicate that MF-1-EPS has good antioxidant activity. Current study demonstrated that MF-1 differed from previously characterized Fusarium sp., and can serve as potential antioxidant. |
format | Online Article Text |
id | pubmed-6127374 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | Elsevier |
record_format | MEDLINE/PubMed |
spelling | pubmed-61273742018-09-07 Chemical characterization and antioxidant properties of exopolysaccharides from mangrove filamentous fungi Fusarium equiseti ANP2 Prathyusha, A.M.V.N. Mohana Sheela, G. Bramhachari, P.V. Biotechnol Rep (Amst) Article A filamentous fungi F. equiseti strain ANP2 with good exopolysaccharide (EPS) yield, apparent viscosity was screened and isolated from Krishna estuarine mangrove sediments. Based on molecular characteristics the selected strain was confirmed as F. equiseti, yielded EPS (4.9 ± 0.25 g/L) and whole cell biomass (3.5 ± 0.2 g/L) in MSM during early stationary phase. The crude EPS was purified by chromatography of Q-Sepharose and Superdex-75, affording Ms and MF-1 fractions were obtained, respectively. Chemical analyses revealed that EPS is primarily composed of neutral sugars and proteins. FTIR-spectroscopy revealed presence of hydroxyl, carbonyl groups and glycosidic bonds which correspond to typical heteropolysaccharide. GC analysis demonstrated that MF-1 mainly consist of mannose (72.6%) and glucose (27.3%). Viscosity studies exhibited typical non-Newtonian pseudoplastic behaviour with high viscosity. The scavenging ability on hydroxyl radicals, indicate that MF-1-EPS has good antioxidant activity. Current study demonstrated that MF-1 differed from previously characterized Fusarium sp., and can serve as potential antioxidant. Elsevier 2018-08-13 /pmc/articles/PMC6127374/ /pubmed/30197873 http://dx.doi.org/10.1016/j.btre.2018.e00277 Text en © 2018 Published by Elsevier B.V. http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). |
spellingShingle | Article Prathyusha, A.M.V.N. Mohana Sheela, G. Bramhachari, P.V. Chemical characterization and antioxidant properties of exopolysaccharides from mangrove filamentous fungi Fusarium equiseti ANP2 |
title | Chemical characterization and antioxidant properties of exopolysaccharides from mangrove filamentous fungi Fusarium equiseti ANP2 |
title_full | Chemical characterization and antioxidant properties of exopolysaccharides from mangrove filamentous fungi Fusarium equiseti ANP2 |
title_fullStr | Chemical characterization and antioxidant properties of exopolysaccharides from mangrove filamentous fungi Fusarium equiseti ANP2 |
title_full_unstemmed | Chemical characterization and antioxidant properties of exopolysaccharides from mangrove filamentous fungi Fusarium equiseti ANP2 |
title_short | Chemical characterization and antioxidant properties of exopolysaccharides from mangrove filamentous fungi Fusarium equiseti ANP2 |
title_sort | chemical characterization and antioxidant properties of exopolysaccharides from mangrove filamentous fungi fusarium equiseti anp2 |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6127374/ https://www.ncbi.nlm.nih.gov/pubmed/30197873 http://dx.doi.org/10.1016/j.btre.2018.e00277 |
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