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Mechanical Mapping of Spinal Cord Growth and Repair in Living Zebrafish Larvae by Brillouin Imaging

The mechanical properties of biological tissues are increasingly recognized as important factors in developmental and pathological processes. Most existing mechanical measurement techniques either necessitate destruction of the tissue for access or provide insufficient spatial resolution. Here, we s...

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Autores principales: Schlüßler, Raimund, Möllmert, Stephanie, Abuhattum, Shada, Cojoc, Gheorghe, Müller, Paul, Kim, Kyoohyun, Möckel, Conrad, Zimmermann, Conrad, Czarske, Jürgen, Guck, Jochen
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Biophysical Society 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6127462/
https://www.ncbi.nlm.nih.gov/pubmed/30122291
http://dx.doi.org/10.1016/j.bpj.2018.07.027
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author Schlüßler, Raimund
Möllmert, Stephanie
Abuhattum, Shada
Cojoc, Gheorghe
Müller, Paul
Kim, Kyoohyun
Möckel, Conrad
Zimmermann, Conrad
Czarske, Jürgen
Guck, Jochen
author_facet Schlüßler, Raimund
Möllmert, Stephanie
Abuhattum, Shada
Cojoc, Gheorghe
Müller, Paul
Kim, Kyoohyun
Möckel, Conrad
Zimmermann, Conrad
Czarske, Jürgen
Guck, Jochen
author_sort Schlüßler, Raimund
collection PubMed
description The mechanical properties of biological tissues are increasingly recognized as important factors in developmental and pathological processes. Most existing mechanical measurement techniques either necessitate destruction of the tissue for access or provide insufficient spatial resolution. Here, we show for the first time to our knowledge a systematic application of confocal Brillouin microscopy to quantitatively map the mechanical properties of spinal cord tissues during biologically relevant processes in a contact-free and nondestructive manner. Living zebrafish larvae were mechanically imaged in all anatomical planes during development and after spinal cord injury. These experiments revealed that Brillouin microscopy is capable of detecting the mechanical properties of distinct anatomical structures without interfering with the animal’s natural development. The Brillouin shift within the spinal cord remained comparable during development and transiently decreased during the repair processes after spinal cord transection. By taking into account the refractive index distribution, we explicitly determined the apparent longitudinal modulus and viscosity of different larval zebrafish tissues. Importantly, mechanical properties differed between tissues in situ and in excised slices. The presented work constitutes the first step toward an in vivo assessment of spinal cord tissue mechanics during regeneration, provides a methodical basis to identify key determinants of mechanical tissue properties, and allows us to test their relative importance in combination with biochemical and genetic factors during developmental and regenerative processes.
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spelling pubmed-61274622019-09-04 Mechanical Mapping of Spinal Cord Growth and Repair in Living Zebrafish Larvae by Brillouin Imaging Schlüßler, Raimund Möllmert, Stephanie Abuhattum, Shada Cojoc, Gheorghe Müller, Paul Kim, Kyoohyun Möckel, Conrad Zimmermann, Conrad Czarske, Jürgen Guck, Jochen Biophys J Systems Biophysics The mechanical properties of biological tissues are increasingly recognized as important factors in developmental and pathological processes. Most existing mechanical measurement techniques either necessitate destruction of the tissue for access or provide insufficient spatial resolution. Here, we show for the first time to our knowledge a systematic application of confocal Brillouin microscopy to quantitatively map the mechanical properties of spinal cord tissues during biologically relevant processes in a contact-free and nondestructive manner. Living zebrafish larvae were mechanically imaged in all anatomical planes during development and after spinal cord injury. These experiments revealed that Brillouin microscopy is capable of detecting the mechanical properties of distinct anatomical structures without interfering with the animal’s natural development. The Brillouin shift within the spinal cord remained comparable during development and transiently decreased during the repair processes after spinal cord transection. By taking into account the refractive index distribution, we explicitly determined the apparent longitudinal modulus and viscosity of different larval zebrafish tissues. Importantly, mechanical properties differed between tissues in situ and in excised slices. The presented work constitutes the first step toward an in vivo assessment of spinal cord tissue mechanics during regeneration, provides a methodical basis to identify key determinants of mechanical tissue properties, and allows us to test their relative importance in combination with biochemical and genetic factors during developmental and regenerative processes. The Biophysical Society 2018-09-04 2018-08-04 /pmc/articles/PMC6127462/ /pubmed/30122291 http://dx.doi.org/10.1016/j.bpj.2018.07.027 Text en © 2018 Biophysical Society. http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Systems Biophysics
Schlüßler, Raimund
Möllmert, Stephanie
Abuhattum, Shada
Cojoc, Gheorghe
Müller, Paul
Kim, Kyoohyun
Möckel, Conrad
Zimmermann, Conrad
Czarske, Jürgen
Guck, Jochen
Mechanical Mapping of Spinal Cord Growth and Repair in Living Zebrafish Larvae by Brillouin Imaging
title Mechanical Mapping of Spinal Cord Growth and Repair in Living Zebrafish Larvae by Brillouin Imaging
title_full Mechanical Mapping of Spinal Cord Growth and Repair in Living Zebrafish Larvae by Brillouin Imaging
title_fullStr Mechanical Mapping of Spinal Cord Growth and Repair in Living Zebrafish Larvae by Brillouin Imaging
title_full_unstemmed Mechanical Mapping of Spinal Cord Growth and Repair in Living Zebrafish Larvae by Brillouin Imaging
title_short Mechanical Mapping of Spinal Cord Growth and Repair in Living Zebrafish Larvae by Brillouin Imaging
title_sort mechanical mapping of spinal cord growth and repair in living zebrafish larvae by brillouin imaging
topic Systems Biophysics
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6127462/
https://www.ncbi.nlm.nih.gov/pubmed/30122291
http://dx.doi.org/10.1016/j.bpj.2018.07.027
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