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A Cell-based Assay to Investigate Non-muscle Myosin II Contractility via the Folded-gastrulation Signaling Pathway in Drosophila S2R+ Cells
We have developed a cell-based assay using Drosophila cells that recapitulates apical constriction initiated by folded gastrulation (Fog), a secreted epithelial morphogen. In this assay, Fog is used as an agonist to activate Rho through a signaling cascade that includes a G-protein-coupled receptor...
Autores principales: | Peters, Kimberly A., Detmar, Elizabeth, Sepulveda, Liz, Del Valle, Corrina, Valsquier, Ruth, Ritz, Anna, Rogers, Stephen L., Applewhite, Derek A. |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MyJove Corporation
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6128210/ https://www.ncbi.nlm.nih.gov/pubmed/30176023 http://dx.doi.org/10.3791/58325 |
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