Cargando…

An LED-based multi-actinic illumination system for the high throughput study of photosynthetic light responses

The responses of photosynthetic organisms to light stress are of interest for both fundamental and applied research. Functional traits related to the photoinhibition, the light-induced loss of photosynthetic efficiency, are particularly interesting as this process is a key limiting factor of photosy...

Descripción completa

Detalles Bibliográficos
Autores principales: Serôdio, João, Schmidt, William, Frommlet, Jörg C., Christa, Gregor, Nitschke, Matthew R.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: PeerJ Inc. 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6128260/
https://www.ncbi.nlm.nih.gov/pubmed/30202661
http://dx.doi.org/10.7717/peerj.5589
_version_ 1783353618478399488
author Serôdio, João
Schmidt, William
Frommlet, Jörg C.
Christa, Gregor
Nitschke, Matthew R.
author_facet Serôdio, João
Schmidt, William
Frommlet, Jörg C.
Christa, Gregor
Nitschke, Matthew R.
author_sort Serôdio, João
collection PubMed
description The responses of photosynthetic organisms to light stress are of interest for both fundamental and applied research. Functional traits related to the photoinhibition, the light-induced loss of photosynthetic efficiency, are particularly interesting as this process is a key limiting factor of photosynthetic productivity in algae and plants. The quantitative characterization of light responses is often time-consuming and calls for cost-effective high throughput approaches that enable the fast screening of multiple samples. Here we present a novel illumination system based on the concept of ‘multi-actinic imaging’ of in vivo chlorophyll fluorescence. The system is based on the combination of an array of individually addressable low power RGBW LEDs and custom-designed well plates, allowing for the independent illumination of 64 samples through the digital manipulation of both exposure duration and light intensity. The illumination system is inexpensive and easily fabricated, based on open source electronics, off-the-shelf components, and 3D-printed parts, and is optimized for imaging of chlorophyll fluorescence. The high-throughput potential of the system is illustrated by assessing the functional diversity in light responses of marine macroalgal species, through the fast and simultaneous determination of kinetic parameters characterizing the response to light stress of multiple samples. Although the presented illumination system was primarily designed for the measurement of phenotypic traits related to photosynthetic activity and photoinhibition, it can be potentially used for a number of alternative applications, including the measurement of chloroplast phototaxis and action spectra, or as the basis for microphotobioreactors.
format Online
Article
Text
id pubmed-6128260
institution National Center for Biotechnology Information
language English
publishDate 2018
publisher PeerJ Inc.
record_format MEDLINE/PubMed
spelling pubmed-61282602018-09-10 An LED-based multi-actinic illumination system for the high throughput study of photosynthetic light responses Serôdio, João Schmidt, William Frommlet, Jörg C. Christa, Gregor Nitschke, Matthew R. PeerJ Ecology The responses of photosynthetic organisms to light stress are of interest for both fundamental and applied research. Functional traits related to the photoinhibition, the light-induced loss of photosynthetic efficiency, are particularly interesting as this process is a key limiting factor of photosynthetic productivity in algae and plants. The quantitative characterization of light responses is often time-consuming and calls for cost-effective high throughput approaches that enable the fast screening of multiple samples. Here we present a novel illumination system based on the concept of ‘multi-actinic imaging’ of in vivo chlorophyll fluorescence. The system is based on the combination of an array of individually addressable low power RGBW LEDs and custom-designed well plates, allowing for the independent illumination of 64 samples through the digital manipulation of both exposure duration and light intensity. The illumination system is inexpensive and easily fabricated, based on open source electronics, off-the-shelf components, and 3D-printed parts, and is optimized for imaging of chlorophyll fluorescence. The high-throughput potential of the system is illustrated by assessing the functional diversity in light responses of marine macroalgal species, through the fast and simultaneous determination of kinetic parameters characterizing the response to light stress of multiple samples. Although the presented illumination system was primarily designed for the measurement of phenotypic traits related to photosynthetic activity and photoinhibition, it can be potentially used for a number of alternative applications, including the measurement of chloroplast phototaxis and action spectra, or as the basis for microphotobioreactors. PeerJ Inc. 2018-09-04 /pmc/articles/PMC6128260/ /pubmed/30202661 http://dx.doi.org/10.7717/peerj.5589 Text en ©2018 Serôdio et al. http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, reproduction and adaptation in any medium and for any purpose provided that it is properly attributed. For attribution, the original author(s), title, publication source (PeerJ) and either DOI or URL of the article must be cited.
spellingShingle Ecology
Serôdio, João
Schmidt, William
Frommlet, Jörg C.
Christa, Gregor
Nitschke, Matthew R.
An LED-based multi-actinic illumination system for the high throughput study of photosynthetic light responses
title An LED-based multi-actinic illumination system for the high throughput study of photosynthetic light responses
title_full An LED-based multi-actinic illumination system for the high throughput study of photosynthetic light responses
title_fullStr An LED-based multi-actinic illumination system for the high throughput study of photosynthetic light responses
title_full_unstemmed An LED-based multi-actinic illumination system for the high throughput study of photosynthetic light responses
title_short An LED-based multi-actinic illumination system for the high throughput study of photosynthetic light responses
title_sort led-based multi-actinic illumination system for the high throughput study of photosynthetic light responses
topic Ecology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6128260/
https://www.ncbi.nlm.nih.gov/pubmed/30202661
http://dx.doi.org/10.7717/peerj.5589
work_keys_str_mv AT serodiojoao anledbasedmultiactinicilluminationsystemforthehighthroughputstudyofphotosyntheticlightresponses
AT schmidtwilliam anledbasedmultiactinicilluminationsystemforthehighthroughputstudyofphotosyntheticlightresponses
AT frommletjorgc anledbasedmultiactinicilluminationsystemforthehighthroughputstudyofphotosyntheticlightresponses
AT christagregor anledbasedmultiactinicilluminationsystemforthehighthroughputstudyofphotosyntheticlightresponses
AT nitschkematthewr anledbasedmultiactinicilluminationsystemforthehighthroughputstudyofphotosyntheticlightresponses
AT serodiojoao ledbasedmultiactinicilluminationsystemforthehighthroughputstudyofphotosyntheticlightresponses
AT schmidtwilliam ledbasedmultiactinicilluminationsystemforthehighthroughputstudyofphotosyntheticlightresponses
AT frommletjorgc ledbasedmultiactinicilluminationsystemforthehighthroughputstudyofphotosyntheticlightresponses
AT christagregor ledbasedmultiactinicilluminationsystemforthehighthroughputstudyofphotosyntheticlightresponses
AT nitschkematthewr ledbasedmultiactinicilluminationsystemforthehighthroughputstudyofphotosyntheticlightresponses