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An LED-based multi-actinic illumination system for the high throughput study of photosynthetic light responses
The responses of photosynthetic organisms to light stress are of interest for both fundamental and applied research. Functional traits related to the photoinhibition, the light-induced loss of photosynthetic efficiency, are particularly interesting as this process is a key limiting factor of photosy...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
PeerJ Inc.
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6128260/ https://www.ncbi.nlm.nih.gov/pubmed/30202661 http://dx.doi.org/10.7717/peerj.5589 |
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author | Serôdio, João Schmidt, William Frommlet, Jörg C. Christa, Gregor Nitschke, Matthew R. |
author_facet | Serôdio, João Schmidt, William Frommlet, Jörg C. Christa, Gregor Nitschke, Matthew R. |
author_sort | Serôdio, João |
collection | PubMed |
description | The responses of photosynthetic organisms to light stress are of interest for both fundamental and applied research. Functional traits related to the photoinhibition, the light-induced loss of photosynthetic efficiency, are particularly interesting as this process is a key limiting factor of photosynthetic productivity in algae and plants. The quantitative characterization of light responses is often time-consuming and calls for cost-effective high throughput approaches that enable the fast screening of multiple samples. Here we present a novel illumination system based on the concept of ‘multi-actinic imaging’ of in vivo chlorophyll fluorescence. The system is based on the combination of an array of individually addressable low power RGBW LEDs and custom-designed well plates, allowing for the independent illumination of 64 samples through the digital manipulation of both exposure duration and light intensity. The illumination system is inexpensive and easily fabricated, based on open source electronics, off-the-shelf components, and 3D-printed parts, and is optimized for imaging of chlorophyll fluorescence. The high-throughput potential of the system is illustrated by assessing the functional diversity in light responses of marine macroalgal species, through the fast and simultaneous determination of kinetic parameters characterizing the response to light stress of multiple samples. Although the presented illumination system was primarily designed for the measurement of phenotypic traits related to photosynthetic activity and photoinhibition, it can be potentially used for a number of alternative applications, including the measurement of chloroplast phototaxis and action spectra, or as the basis for microphotobioreactors. |
format | Online Article Text |
id | pubmed-6128260 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | PeerJ Inc. |
record_format | MEDLINE/PubMed |
spelling | pubmed-61282602018-09-10 An LED-based multi-actinic illumination system for the high throughput study of photosynthetic light responses Serôdio, João Schmidt, William Frommlet, Jörg C. Christa, Gregor Nitschke, Matthew R. PeerJ Ecology The responses of photosynthetic organisms to light stress are of interest for both fundamental and applied research. Functional traits related to the photoinhibition, the light-induced loss of photosynthetic efficiency, are particularly interesting as this process is a key limiting factor of photosynthetic productivity in algae and plants. The quantitative characterization of light responses is often time-consuming and calls for cost-effective high throughput approaches that enable the fast screening of multiple samples. Here we present a novel illumination system based on the concept of ‘multi-actinic imaging’ of in vivo chlorophyll fluorescence. The system is based on the combination of an array of individually addressable low power RGBW LEDs and custom-designed well plates, allowing for the independent illumination of 64 samples through the digital manipulation of both exposure duration and light intensity. The illumination system is inexpensive and easily fabricated, based on open source electronics, off-the-shelf components, and 3D-printed parts, and is optimized for imaging of chlorophyll fluorescence. The high-throughput potential of the system is illustrated by assessing the functional diversity in light responses of marine macroalgal species, through the fast and simultaneous determination of kinetic parameters characterizing the response to light stress of multiple samples. Although the presented illumination system was primarily designed for the measurement of phenotypic traits related to photosynthetic activity and photoinhibition, it can be potentially used for a number of alternative applications, including the measurement of chloroplast phototaxis and action spectra, or as the basis for microphotobioreactors. PeerJ Inc. 2018-09-04 /pmc/articles/PMC6128260/ /pubmed/30202661 http://dx.doi.org/10.7717/peerj.5589 Text en ©2018 Serôdio et al. http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, reproduction and adaptation in any medium and for any purpose provided that it is properly attributed. For attribution, the original author(s), title, publication source (PeerJ) and either DOI or URL of the article must be cited. |
spellingShingle | Ecology Serôdio, João Schmidt, William Frommlet, Jörg C. Christa, Gregor Nitschke, Matthew R. An LED-based multi-actinic illumination system for the high throughput study of photosynthetic light responses |
title | An LED-based multi-actinic illumination system for the high throughput study of photosynthetic light responses |
title_full | An LED-based multi-actinic illumination system for the high throughput study of photosynthetic light responses |
title_fullStr | An LED-based multi-actinic illumination system for the high throughput study of photosynthetic light responses |
title_full_unstemmed | An LED-based multi-actinic illumination system for the high throughput study of photosynthetic light responses |
title_short | An LED-based multi-actinic illumination system for the high throughput study of photosynthetic light responses |
title_sort | led-based multi-actinic illumination system for the high throughput study of photosynthetic light responses |
topic | Ecology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6128260/ https://www.ncbi.nlm.nih.gov/pubmed/30202661 http://dx.doi.org/10.7717/peerj.5589 |
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