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Gender- and region-specific changes in estrogen signaling in aging rat brain mitochondria
Recently epidemiological studies suggest females lose neuroprotection from neurodegenerative diseases as they go through menopause. It has been hypothesized that this neuroprotection is hormone-dependent. The current study characterized cell signaling molecules downstream of estrogen receptor beta t...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Impact Journals
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6128413/ https://www.ncbi.nlm.nih.gov/pubmed/30169330 http://dx.doi.org/10.18632/aging.101538 |
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author | Evola, Christopher M. Hudson, Tanner L. Huang, Luping Corbett, Adrian M. Mayes, Debra A. |
author_facet | Evola, Christopher M. Hudson, Tanner L. Huang, Luping Corbett, Adrian M. Mayes, Debra A. |
author_sort | Evola, Christopher M. |
collection | PubMed |
description | Recently epidemiological studies suggest females lose neuroprotection from neurodegenerative diseases as they go through menopause. It has been hypothesized that this neuroprotection is hormone-dependent. The current study characterized cell signaling molecules downstream of estrogen receptor beta that are known to play a role in memory, PKC, ERK, and connexin-43, in regions of the brain associated with memory decline in an attempt to elucidate significant changes that occur post-estrus. Total whole cell lysates were compared to isolated mitochondrial protein because mitochondrial function is known to be altered during aging. As hypothesized, protein concentrations differed depending on age, gender, and brain region. Additionally, many of these changes occurred within mitochondria but not within whole cell lysates indicating that these are epigenetic alterations. These findings accentuate the complexity of aging and provide insight into the gender-specific cellular processes that occur throughout this process. |
format | Online Article Text |
id | pubmed-6128413 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | Impact Journals |
record_format | MEDLINE/PubMed |
spelling | pubmed-61284132018-09-10 Gender- and region-specific changes in estrogen signaling in aging rat brain mitochondria Evola, Christopher M. Hudson, Tanner L. Huang, Luping Corbett, Adrian M. Mayes, Debra A. Aging (Albany NY) Research Paper Recently epidemiological studies suggest females lose neuroprotection from neurodegenerative diseases as they go through menopause. It has been hypothesized that this neuroprotection is hormone-dependent. The current study characterized cell signaling molecules downstream of estrogen receptor beta that are known to play a role in memory, PKC, ERK, and connexin-43, in regions of the brain associated with memory decline in an attempt to elucidate significant changes that occur post-estrus. Total whole cell lysates were compared to isolated mitochondrial protein because mitochondrial function is known to be altered during aging. As hypothesized, protein concentrations differed depending on age, gender, and brain region. Additionally, many of these changes occurred within mitochondria but not within whole cell lysates indicating that these are epigenetic alterations. These findings accentuate the complexity of aging and provide insight into the gender-specific cellular processes that occur throughout this process. Impact Journals 2018-08-31 /pmc/articles/PMC6128413/ /pubmed/30169330 http://dx.doi.org/10.18632/aging.101538 Text en Copyright © 2018 Evola et al. http://creativecommons.org/licenses/by/3.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution (CC BY) 3.0 License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Paper Evola, Christopher M. Hudson, Tanner L. Huang, Luping Corbett, Adrian M. Mayes, Debra A. Gender- and region-specific changes in estrogen signaling in aging rat brain mitochondria |
title | Gender- and region-specific changes in estrogen signaling in aging rat brain mitochondria |
title_full | Gender- and region-specific changes in estrogen signaling in aging rat brain mitochondria |
title_fullStr | Gender- and region-specific changes in estrogen signaling in aging rat brain mitochondria |
title_full_unstemmed | Gender- and region-specific changes in estrogen signaling in aging rat brain mitochondria |
title_short | Gender- and region-specific changes in estrogen signaling in aging rat brain mitochondria |
title_sort | gender- and region-specific changes in estrogen signaling in aging rat brain mitochondria |
topic | Research Paper |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6128413/ https://www.ncbi.nlm.nih.gov/pubmed/30169330 http://dx.doi.org/10.18632/aging.101538 |
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