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Increases in plasma corin levels following experimental myocardial infarction reflect the severity of ischemic injury

Following acute myocardial infarction, clinical studies show alterations in the blood levels of corin, a cardiac-selective activator of the natriuretic peptides pro-atrial natriuretic peptide (pro-ANP) and pro-B-type natriuretic peptide (pro-BNP). However, the temporal changes in circulating and car...

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Autores principales: Wang, Dong, Gladysheva, Inna P., Sullivan, Ryan D., Fan, Tai-Hwang M., Mehta, Radhika M., Tripathi, Ranjana, Sun, Yao, Reed, Guy L.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6128455/
https://www.ncbi.nlm.nih.gov/pubmed/30192780
http://dx.doi.org/10.1371/journal.pone.0202571
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author Wang, Dong
Gladysheva, Inna P.
Sullivan, Ryan D.
Fan, Tai-Hwang M.
Mehta, Radhika M.
Tripathi, Ranjana
Sun, Yao
Reed, Guy L.
author_facet Wang, Dong
Gladysheva, Inna P.
Sullivan, Ryan D.
Fan, Tai-Hwang M.
Mehta, Radhika M.
Tripathi, Ranjana
Sun, Yao
Reed, Guy L.
author_sort Wang, Dong
collection PubMed
description Following acute myocardial infarction, clinical studies show alterations in the blood levels of corin, a cardiac-selective activator of the natriuretic peptides pro-atrial natriuretic peptide (pro-ANP) and pro-B-type natriuretic peptide (pro-BNP). However, the temporal changes in circulating and cardiac corin levels and their relationships to the severity of myocardial infarction have not been studied. The main objective of this study was to examine the relationship between cardiac and circulating corin levels and their association with cardiac systolic function and infarct size during the early phase of acute myocardial infarction (<72 h) in a translationally relevant induced coronary ligation mouse model. This acute phase timeline was chosen to correlate with the clinical practice within which blood samples are collected from myocardial infarction patients. Heart and plasma samples were examined at 3, 24, and 72 hours post acute myocardial infarction. Plasma corin levels were examined by enzyme-linked immunosorbent assay, transcripts of cardiac corin, pro-ANP and pro-BNP by quantitative real-time polymerase chain reaction, cardiac corin expression by immunohistology, infarct size by histology and heart function by echocardiography. Plasma corin levels were significantly increased at 3 (P<0.05), 24 (P<0.001), and 72 hours (P<0.01) post-acute myocardial infarction. In contrast, cardiac corin transcript levels dropped by 5% (P>0.05), 69% (P<0.001) and 65% (P<0.001) and immunoreactive cardiac corin protein levels dropped by 30% (P<0.05), 76% (P<0.001) and 75% (P<0.001), while cardiac pro-ANP and pro-BNP transcript levels showed an opposite pattern. Plasma corin levels were negatively correlated with immunoreactive cardiac corin (P<0.01), ejection fraction (P<0.05) and fractional shortening (P<0.05), but positively correlated with infarct size (P<0.01). In conclusion, acute myocardial infarction induces rapid increases in plasma corin and decreases in cardiac corin levels. In the early phase of acute myocardial infarction, plasma corin levels are inversely correlated with heart function and may reflect the severity of myocardial damage.
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spelling pubmed-61284552018-09-15 Increases in plasma corin levels following experimental myocardial infarction reflect the severity of ischemic injury Wang, Dong Gladysheva, Inna P. Sullivan, Ryan D. Fan, Tai-Hwang M. Mehta, Radhika M. Tripathi, Ranjana Sun, Yao Reed, Guy L. PLoS One Research Article Following acute myocardial infarction, clinical studies show alterations in the blood levels of corin, a cardiac-selective activator of the natriuretic peptides pro-atrial natriuretic peptide (pro-ANP) and pro-B-type natriuretic peptide (pro-BNP). However, the temporal changes in circulating and cardiac corin levels and their relationships to the severity of myocardial infarction have not been studied. The main objective of this study was to examine the relationship between cardiac and circulating corin levels and their association with cardiac systolic function and infarct size during the early phase of acute myocardial infarction (<72 h) in a translationally relevant induced coronary ligation mouse model. This acute phase timeline was chosen to correlate with the clinical practice within which blood samples are collected from myocardial infarction patients. Heart and plasma samples were examined at 3, 24, and 72 hours post acute myocardial infarction. Plasma corin levels were examined by enzyme-linked immunosorbent assay, transcripts of cardiac corin, pro-ANP and pro-BNP by quantitative real-time polymerase chain reaction, cardiac corin expression by immunohistology, infarct size by histology and heart function by echocardiography. Plasma corin levels were significantly increased at 3 (P<0.05), 24 (P<0.001), and 72 hours (P<0.01) post-acute myocardial infarction. In contrast, cardiac corin transcript levels dropped by 5% (P>0.05), 69% (P<0.001) and 65% (P<0.001) and immunoreactive cardiac corin protein levels dropped by 30% (P<0.05), 76% (P<0.001) and 75% (P<0.001), while cardiac pro-ANP and pro-BNP transcript levels showed an opposite pattern. Plasma corin levels were negatively correlated with immunoreactive cardiac corin (P<0.01), ejection fraction (P<0.05) and fractional shortening (P<0.05), but positively correlated with infarct size (P<0.01). In conclusion, acute myocardial infarction induces rapid increases in plasma corin and decreases in cardiac corin levels. In the early phase of acute myocardial infarction, plasma corin levels are inversely correlated with heart function and may reflect the severity of myocardial damage. Public Library of Science 2018-09-07 /pmc/articles/PMC6128455/ /pubmed/30192780 http://dx.doi.org/10.1371/journal.pone.0202571 Text en © 2018 Wang et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Wang, Dong
Gladysheva, Inna P.
Sullivan, Ryan D.
Fan, Tai-Hwang M.
Mehta, Radhika M.
Tripathi, Ranjana
Sun, Yao
Reed, Guy L.
Increases in plasma corin levels following experimental myocardial infarction reflect the severity of ischemic injury
title Increases in plasma corin levels following experimental myocardial infarction reflect the severity of ischemic injury
title_full Increases in plasma corin levels following experimental myocardial infarction reflect the severity of ischemic injury
title_fullStr Increases in plasma corin levels following experimental myocardial infarction reflect the severity of ischemic injury
title_full_unstemmed Increases in plasma corin levels following experimental myocardial infarction reflect the severity of ischemic injury
title_short Increases in plasma corin levels following experimental myocardial infarction reflect the severity of ischemic injury
title_sort increases in plasma corin levels following experimental myocardial infarction reflect the severity of ischemic injury
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6128455/
https://www.ncbi.nlm.nih.gov/pubmed/30192780
http://dx.doi.org/10.1371/journal.pone.0202571
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