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Detection and characterization of Lactobacillus spp. in the porcine seminal plasma and their influence on boar semen quality
The presence of pathogenic bacteria in ejaculates has been a topic in boar semen preservation over the last decades. Since little information is available on commensal bacteria in boar semen, the aim of the present study was to identify commensal lactobacilli in fresh cryopreserved boar semen and to...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Public Library of Science
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6128545/ https://www.ncbi.nlm.nih.gov/pubmed/30192779 http://dx.doi.org/10.1371/journal.pone.0202699 |
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author | Schulze, Martin Schäfer, Jana Simmet, Christian Jung, Markus Gabler, Christoph |
author_facet | Schulze, Martin Schäfer, Jana Simmet, Christian Jung, Markus Gabler, Christoph |
author_sort | Schulze, Martin |
collection | PubMed |
description | The presence of pathogenic bacteria in ejaculates has been a topic in boar semen preservation over the last decades. Since little information is available on commensal bacteria in boar semen, the aim of the present study was to identify commensal lactobacilli in fresh cryopreserved boar semen and to examine their influence on boar semen quality. Therefore, 111 boar ejaculates were investigated for the presence of Lactobacillus species. Thirty samples (27%) contained viable Lactobacillus species (e.g. L. amylovorus, L. animalis, L. reuteri and Weisella minor). L. animalis and L. buchneri DSM 32407 (isolated from the bovine uterus) qualified for further examinations based on their growth rate in six antibiotic-free boar semen extenders. After a 120 min short-term incubation with an antibiotic-free BTS-extender, progressive motility was diminished (P = 0.001) upon addition of 10(5) and 10(6) colony forming units (CFU/mL) L. animalis. The supplementation with L. buchneri DSM 32407 had no significant (P > 0.05) influence on sperm quality during short-term co-incubation. After 168 h long-term co-incubation, motility analysis revealed a negative (P = 0.026) impact of 10(5) CFU/mL L. buchneri DSM 32407. A concentration- and storage-dependent effect is particularly obvious (P < 0.001) using 10(6) CFU/mL L. buchneri DSM 32407. Most notably, the thermo-resistance (TRT) for 10(6) CFU/mL L. buchneri DSM 32407 (P = 0.001) was inferior to BTS with and without gentamicin after 72 and 168 h of semen co-incubation. The supplementation of 10(5) CFU/mL L. buchneri DSM 32407 impaired progressive motility to a lesser extent. The percentage of mitochondrially active spermatozoa after 96 h (P = 0.009) and membrane-intact spermatozoa after 168 h (P < 0.001) was lower when 10(6) CFU/mL L. buchneri DSM 32407 were suspended compared with all other groups. Finally, the addition of L. buchneri DSM 32407 to BTS-extended boar semen had no competitive effect on the total amount of bacteria 48 h after co-incubation. In summary, the present study demonstrated that there are Lactobacillus species present in the porcine seminal plasma, which can be cultivated using standard procedures. However, long-term co-incubation of lactic acid bacteria with spermatozoa had a negative influence on spermatozoa. |
format | Online Article Text |
id | pubmed-6128545 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-61285452018-09-15 Detection and characterization of Lactobacillus spp. in the porcine seminal plasma and their influence on boar semen quality Schulze, Martin Schäfer, Jana Simmet, Christian Jung, Markus Gabler, Christoph PLoS One Research Article The presence of pathogenic bacteria in ejaculates has been a topic in boar semen preservation over the last decades. Since little information is available on commensal bacteria in boar semen, the aim of the present study was to identify commensal lactobacilli in fresh cryopreserved boar semen and to examine their influence on boar semen quality. Therefore, 111 boar ejaculates were investigated for the presence of Lactobacillus species. Thirty samples (27%) contained viable Lactobacillus species (e.g. L. amylovorus, L. animalis, L. reuteri and Weisella minor). L. animalis and L. buchneri DSM 32407 (isolated from the bovine uterus) qualified for further examinations based on their growth rate in six antibiotic-free boar semen extenders. After a 120 min short-term incubation with an antibiotic-free BTS-extender, progressive motility was diminished (P = 0.001) upon addition of 10(5) and 10(6) colony forming units (CFU/mL) L. animalis. The supplementation with L. buchneri DSM 32407 had no significant (P > 0.05) influence on sperm quality during short-term co-incubation. After 168 h long-term co-incubation, motility analysis revealed a negative (P = 0.026) impact of 10(5) CFU/mL L. buchneri DSM 32407. A concentration- and storage-dependent effect is particularly obvious (P < 0.001) using 10(6) CFU/mL L. buchneri DSM 32407. Most notably, the thermo-resistance (TRT) for 10(6) CFU/mL L. buchneri DSM 32407 (P = 0.001) was inferior to BTS with and without gentamicin after 72 and 168 h of semen co-incubation. The supplementation of 10(5) CFU/mL L. buchneri DSM 32407 impaired progressive motility to a lesser extent. The percentage of mitochondrially active spermatozoa after 96 h (P = 0.009) and membrane-intact spermatozoa after 168 h (P < 0.001) was lower when 10(6) CFU/mL L. buchneri DSM 32407 were suspended compared with all other groups. Finally, the addition of L. buchneri DSM 32407 to BTS-extended boar semen had no competitive effect on the total amount of bacteria 48 h after co-incubation. In summary, the present study demonstrated that there are Lactobacillus species present in the porcine seminal plasma, which can be cultivated using standard procedures. However, long-term co-incubation of lactic acid bacteria with spermatozoa had a negative influence on spermatozoa. Public Library of Science 2018-09-07 /pmc/articles/PMC6128545/ /pubmed/30192779 http://dx.doi.org/10.1371/journal.pone.0202699 Text en © 2018 Schulze et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Article Schulze, Martin Schäfer, Jana Simmet, Christian Jung, Markus Gabler, Christoph Detection and characterization of Lactobacillus spp. in the porcine seminal plasma and their influence on boar semen quality |
title | Detection and characterization of Lactobacillus spp. in the porcine seminal plasma and their influence on boar semen quality |
title_full | Detection and characterization of Lactobacillus spp. in the porcine seminal plasma and their influence on boar semen quality |
title_fullStr | Detection and characterization of Lactobacillus spp. in the porcine seminal plasma and their influence on boar semen quality |
title_full_unstemmed | Detection and characterization of Lactobacillus spp. in the porcine seminal plasma and their influence on boar semen quality |
title_short | Detection and characterization of Lactobacillus spp. in the porcine seminal plasma and their influence on boar semen quality |
title_sort | detection and characterization of lactobacillus spp. in the porcine seminal plasma and their influence on boar semen quality |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6128545/ https://www.ncbi.nlm.nih.gov/pubmed/30192779 http://dx.doi.org/10.1371/journal.pone.0202699 |
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