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Integrative Analysis of lncRNAs in Th17 Cell Lineage to Discover New Potential Biomarkers and Therapeutic Targets in Autoimmune Diseases
Th17 cells play a critical role in the pathogenesis of autoimmune diseases, including multiple sclerosis, rheumatoid arthritis, systemic lupus erythematosus, Sjogren’s syndrome, and inflammatory bowel disease. Despite the extensive investigation into this T cell lineage, little is understood regardi...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
American Society of Gene & Cell Therapy
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6128809/ https://www.ncbi.nlm.nih.gov/pubmed/30195777 http://dx.doi.org/10.1016/j.omtn.2018.05.022 |
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author | Teimuri, Shohreh Hosseini, Aref Rezaenasab, Ahmad Ghaedi, Kamran Ghoveud, Elahe Etemadifar, Masoud Nasr-Esfahani, Mohammad Hossein Megraw, Timothy L. |
author_facet | Teimuri, Shohreh Hosseini, Aref Rezaenasab, Ahmad Ghaedi, Kamran Ghoveud, Elahe Etemadifar, Masoud Nasr-Esfahani, Mohammad Hossein Megraw, Timothy L. |
author_sort | Teimuri, Shohreh |
collection | PubMed |
description | Th17 cells play a critical role in the pathogenesis of autoimmune diseases, including multiple sclerosis, rheumatoid arthritis, systemic lupus erythematosus, Sjogren’s syndrome, and inflammatory bowel disease. Despite the extensive investigation into this T cell lineage, little is understood regarding the role of Th17 lineage-specific lncRNAs (long non-coding RNAs) > 200 nt. lncRNAs may influence disease through a variety of mechanisms; their expression could be regulated by SNPs. lncRNAs can also affect the expression of neighboring genes or complementary miRNAs, and their expression may have lineage-specific patterns. In the system biology study presented here, the effective lncRNAs from different criteria were predicted for each autoimmune disease, and we then evaluated their expression levels in 50 MS patients compared to 25 controls using qRT-PCR. We identified changes in the expression levels of AL450992.2, AC009948.5, and RP11-98D18.3 as potential peripheral blood mononuclear cell (PBMC) biomarkers for MS among our studied lncRNAs in which co-expression analysis of AL450992.2 had the most AUCs, and the relationship to RORC was also assessed. We propose that the recurrently deregulated lncRNAs identified in this report could provide a valuable resource for studies aimed at delineating the relationship between functional lncRNAs and autoimmune disorders. |
format | Online Article Text |
id | pubmed-6128809 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | American Society of Gene & Cell Therapy |
record_format | MEDLINE/PubMed |
spelling | pubmed-61288092018-09-10 Integrative Analysis of lncRNAs in Th17 Cell Lineage to Discover New Potential Biomarkers and Therapeutic Targets in Autoimmune Diseases Teimuri, Shohreh Hosseini, Aref Rezaenasab, Ahmad Ghaedi, Kamran Ghoveud, Elahe Etemadifar, Masoud Nasr-Esfahani, Mohammad Hossein Megraw, Timothy L. Mol Ther Nucleic Acids Article Th17 cells play a critical role in the pathogenesis of autoimmune diseases, including multiple sclerosis, rheumatoid arthritis, systemic lupus erythematosus, Sjogren’s syndrome, and inflammatory bowel disease. Despite the extensive investigation into this T cell lineage, little is understood regarding the role of Th17 lineage-specific lncRNAs (long non-coding RNAs) > 200 nt. lncRNAs may influence disease through a variety of mechanisms; their expression could be regulated by SNPs. lncRNAs can also affect the expression of neighboring genes or complementary miRNAs, and their expression may have lineage-specific patterns. In the system biology study presented here, the effective lncRNAs from different criteria were predicted for each autoimmune disease, and we then evaluated their expression levels in 50 MS patients compared to 25 controls using qRT-PCR. We identified changes in the expression levels of AL450992.2, AC009948.5, and RP11-98D18.3 as potential peripheral blood mononuclear cell (PBMC) biomarkers for MS among our studied lncRNAs in which co-expression analysis of AL450992.2 had the most AUCs, and the relationship to RORC was also assessed. We propose that the recurrently deregulated lncRNAs identified in this report could provide a valuable resource for studies aimed at delineating the relationship between functional lncRNAs and autoimmune disorders. American Society of Gene & Cell Therapy 2018-07-11 /pmc/articles/PMC6128809/ /pubmed/30195777 http://dx.doi.org/10.1016/j.omtn.2018.05.022 Text en © 2018 The Authors http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). |
spellingShingle | Article Teimuri, Shohreh Hosseini, Aref Rezaenasab, Ahmad Ghaedi, Kamran Ghoveud, Elahe Etemadifar, Masoud Nasr-Esfahani, Mohammad Hossein Megraw, Timothy L. Integrative Analysis of lncRNAs in Th17 Cell Lineage to Discover New Potential Biomarkers and Therapeutic Targets in Autoimmune Diseases |
title | Integrative Analysis of lncRNAs in Th17 Cell Lineage to Discover New Potential Biomarkers and Therapeutic Targets in Autoimmune Diseases |
title_full | Integrative Analysis of lncRNAs in Th17 Cell Lineage to Discover New Potential Biomarkers and Therapeutic Targets in Autoimmune Diseases |
title_fullStr | Integrative Analysis of lncRNAs in Th17 Cell Lineage to Discover New Potential Biomarkers and Therapeutic Targets in Autoimmune Diseases |
title_full_unstemmed | Integrative Analysis of lncRNAs in Th17 Cell Lineage to Discover New Potential Biomarkers and Therapeutic Targets in Autoimmune Diseases |
title_short | Integrative Analysis of lncRNAs in Th17 Cell Lineage to Discover New Potential Biomarkers and Therapeutic Targets in Autoimmune Diseases |
title_sort | integrative analysis of lncrnas in th17 cell lineage to discover new potential biomarkers and therapeutic targets in autoimmune diseases |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6128809/ https://www.ncbi.nlm.nih.gov/pubmed/30195777 http://dx.doi.org/10.1016/j.omtn.2018.05.022 |
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