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Selection of Tumor-Specific Cytotoxic T Lymphocytes in Acute Myeloid Leukemia Patients Through the Identification of T-Cells Capable to Establish Stable Interactions With the Leukemic Cells: “Doublet Technology”
The relevance of the immune system in cancer has long been studied. Autologous adoptive T cell therapies, based on the use of tumor infiltrating lymphocytes (TILs), have made great progress in recent years for the treatment of solid tumors, especially melanoma. However, further work is needed to iso...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Frontiers Media S.A.
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6129592/ https://www.ncbi.nlm.nih.gov/pubmed/30233577 http://dx.doi.org/10.3389/fimmu.2018.01971 |
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author | García-Guerrero, Estefanía Sánchez-Abarca, Luís I. Domingo, Esther Ramos, Teresa L. Bejarano-García, Jose A. Gonzalez-Campos, Jose A. Caballero-Velázquez, Teresa Pérez-Simón, Jose A. |
author_facet | García-Guerrero, Estefanía Sánchez-Abarca, Luís I. Domingo, Esther Ramos, Teresa L. Bejarano-García, Jose A. Gonzalez-Campos, Jose A. Caballero-Velázquez, Teresa Pérez-Simón, Jose A. |
author_sort | García-Guerrero, Estefanía |
collection | PubMed |
description | The relevance of the immune system in cancer has long been studied. Autologous adoptive T cell therapies, based on the use of tumor infiltrating lymphocytes (TILs), have made great progress in recent years for the treatment of solid tumors, especially melanoma. However, further work is needed to isolate tumor-reactive T cells among patients diagnosed with hematologic malignancies. The dynamics of the interaction between T cells and antigen presenting cells (APC) dictate the quality of the immune responses. While stable joints between target cells and T lymphocytes lead to the induction of T cell activation and immune response, brief contacts contribute to the induction of immune-tolerance. Taking advantage of the strong interaction between target cell and activated T-cells, we show the feasibility to identify and isolate tumor-specific cytotoxic T lymphocytes (CTLs) from acute myeloid leukemia (AML) patients by flow cytometry. Using this technology, CTLs bound through T cell receptor (TCR) to tumor cells can be identified in peripheral blood and bone marrow and subsequently selected and isolated by FACS-based cell sorting. These CTLs display higher percentage of effector cells and marked cytotoxic activity against AML blasts. In conclusion, we have developed a new procedure to identify and select specific cytotoxic T cells in patients diagnosed with acute myeloid leukemia. |
format | Online Article Text |
id | pubmed-6129592 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-61295922018-09-19 Selection of Tumor-Specific Cytotoxic T Lymphocytes in Acute Myeloid Leukemia Patients Through the Identification of T-Cells Capable to Establish Stable Interactions With the Leukemic Cells: “Doublet Technology” García-Guerrero, Estefanía Sánchez-Abarca, Luís I. Domingo, Esther Ramos, Teresa L. Bejarano-García, Jose A. Gonzalez-Campos, Jose A. Caballero-Velázquez, Teresa Pérez-Simón, Jose A. Front Immunol Immunology The relevance of the immune system in cancer has long been studied. Autologous adoptive T cell therapies, based on the use of tumor infiltrating lymphocytes (TILs), have made great progress in recent years for the treatment of solid tumors, especially melanoma. However, further work is needed to isolate tumor-reactive T cells among patients diagnosed with hematologic malignancies. The dynamics of the interaction between T cells and antigen presenting cells (APC) dictate the quality of the immune responses. While stable joints between target cells and T lymphocytes lead to the induction of T cell activation and immune response, brief contacts contribute to the induction of immune-tolerance. Taking advantage of the strong interaction between target cell and activated T-cells, we show the feasibility to identify and isolate tumor-specific cytotoxic T lymphocytes (CTLs) from acute myeloid leukemia (AML) patients by flow cytometry. Using this technology, CTLs bound through T cell receptor (TCR) to tumor cells can be identified in peripheral blood and bone marrow and subsequently selected and isolated by FACS-based cell sorting. These CTLs display higher percentage of effector cells and marked cytotoxic activity against AML blasts. In conclusion, we have developed a new procedure to identify and select specific cytotoxic T cells in patients diagnosed with acute myeloid leukemia. Frontiers Media S.A. 2018-09-03 /pmc/articles/PMC6129592/ /pubmed/30233577 http://dx.doi.org/10.3389/fimmu.2018.01971 Text en Copyright © 2018 García-Guerrero, Sánchez-Abarca, Domingo, Ramos, Bejarano-García, Gonzalez-Campos, Caballero-Velázquez and Pérez-Simón. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Immunology García-Guerrero, Estefanía Sánchez-Abarca, Luís I. Domingo, Esther Ramos, Teresa L. Bejarano-García, Jose A. Gonzalez-Campos, Jose A. Caballero-Velázquez, Teresa Pérez-Simón, Jose A. Selection of Tumor-Specific Cytotoxic T Lymphocytes in Acute Myeloid Leukemia Patients Through the Identification of T-Cells Capable to Establish Stable Interactions With the Leukemic Cells: “Doublet Technology” |
title | Selection of Tumor-Specific Cytotoxic T Lymphocytes in Acute Myeloid Leukemia Patients Through the Identification of T-Cells Capable to Establish Stable Interactions With the Leukemic Cells: “Doublet Technology” |
title_full | Selection of Tumor-Specific Cytotoxic T Lymphocytes in Acute Myeloid Leukemia Patients Through the Identification of T-Cells Capable to Establish Stable Interactions With the Leukemic Cells: “Doublet Technology” |
title_fullStr | Selection of Tumor-Specific Cytotoxic T Lymphocytes in Acute Myeloid Leukemia Patients Through the Identification of T-Cells Capable to Establish Stable Interactions With the Leukemic Cells: “Doublet Technology” |
title_full_unstemmed | Selection of Tumor-Specific Cytotoxic T Lymphocytes in Acute Myeloid Leukemia Patients Through the Identification of T-Cells Capable to Establish Stable Interactions With the Leukemic Cells: “Doublet Technology” |
title_short | Selection of Tumor-Specific Cytotoxic T Lymphocytes in Acute Myeloid Leukemia Patients Through the Identification of T-Cells Capable to Establish Stable Interactions With the Leukemic Cells: “Doublet Technology” |
title_sort | selection of tumor-specific cytotoxic t lymphocytes in acute myeloid leukemia patients through the identification of t-cells capable to establish stable interactions with the leukemic cells: “doublet technology” |
topic | Immunology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6129592/ https://www.ncbi.nlm.nih.gov/pubmed/30233577 http://dx.doi.org/10.3389/fimmu.2018.01971 |
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