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Effective Antimicrobial Activity of Green ZnO Nano Particles of Catharanthus roseus
In the present study, zinc oxide nanoparticles (ZnO NPs) were synthesized using leaf extract of Catharanthus roseus (C. roseus) under different physical parameters. Biosynthesis of ZnO NPs was confirmed by UV-Visible spectrophotometer and further, characterized by X-Ray Diffraction (XRD), Fourier Tr...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Frontiers Media S.A.
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6129596/ https://www.ncbi.nlm.nih.gov/pubmed/30233518 http://dx.doi.org/10.3389/fmicb.2018.02030 |
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author | Gupta, Monika Tomar, Rajesh S. Kaushik, Shuchi Mishra, Raghvendra K. Sharma, Divakar |
author_facet | Gupta, Monika Tomar, Rajesh S. Kaushik, Shuchi Mishra, Raghvendra K. Sharma, Divakar |
author_sort | Gupta, Monika |
collection | PubMed |
description | In the present study, zinc oxide nanoparticles (ZnO NPs) were synthesized using leaf extract of Catharanthus roseus (C. roseus) under different physical parameters. Biosynthesis of ZnO NPs was confirmed by UV-Visible spectrophotometer and further, characterized by X-Ray Diffraction (XRD), Fourier Transform Infrared spectroscopy (FTIR), Scanning Electron Microscopy (SEM), Transmission Electron Microscopy (TEM), Energy-Dispersive X-ray spectroscopy (EDX), Atomic Force Microscopy (AFM), Photoluminescence study and Dynamic Light Scattering (DLS). We have also confirmed that several physical parameters such as pH, temperature, concentration of metal ions and reaction time were able to regulate shape and size of synthesized ZnO NPs. XRD and TEM analysis provided the information about the average size and hexagonal morphology of ZnO NPs. FTIR spectra analysis suggested that phenolic compounds played crucial role in the biosynthesis of ZnO NPs. The significant antibacterial activity of ZnO NPs was observed against Staphylococcus aureus MTCC 9760 (S. aureus), Streptococcus pyogenes MTCC 1926 (S. pyogenes), Bacillus cereus MTCC 430 (B. cereus), Pseudomonas aeruginosa MTCC 424 (P. aeruginosa), Proteus mirabilis MTCC 3310 (P. mirabilis) and Escherichia coli MTCC 40 (E. coli). The synthesized ZnO NPs have shown antibacterial efficacy against both Gram-positive and Gram-negative pathogens. Synergistic effects of ZnO NPs and streptomycin showed increased efficacy as indicated by the increased zone of clearance in comparison to their individual effects (either ZnO NPs or streptomycin). Overall, the results elucidated a rapid, cost-effective, environmentally friendly and convenient method for ZnO NPs synthesis, which could be used as a potential antimicrobial agent against drug resistant microbes. |
format | Online Article Text |
id | pubmed-6129596 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-61295962018-09-19 Effective Antimicrobial Activity of Green ZnO Nano Particles of Catharanthus roseus Gupta, Monika Tomar, Rajesh S. Kaushik, Shuchi Mishra, Raghvendra K. Sharma, Divakar Front Microbiol Microbiology In the present study, zinc oxide nanoparticles (ZnO NPs) were synthesized using leaf extract of Catharanthus roseus (C. roseus) under different physical parameters. Biosynthesis of ZnO NPs was confirmed by UV-Visible spectrophotometer and further, characterized by X-Ray Diffraction (XRD), Fourier Transform Infrared spectroscopy (FTIR), Scanning Electron Microscopy (SEM), Transmission Electron Microscopy (TEM), Energy-Dispersive X-ray spectroscopy (EDX), Atomic Force Microscopy (AFM), Photoluminescence study and Dynamic Light Scattering (DLS). We have also confirmed that several physical parameters such as pH, temperature, concentration of metal ions and reaction time were able to regulate shape and size of synthesized ZnO NPs. XRD and TEM analysis provided the information about the average size and hexagonal morphology of ZnO NPs. FTIR spectra analysis suggested that phenolic compounds played crucial role in the biosynthesis of ZnO NPs. The significant antibacterial activity of ZnO NPs was observed against Staphylococcus aureus MTCC 9760 (S. aureus), Streptococcus pyogenes MTCC 1926 (S. pyogenes), Bacillus cereus MTCC 430 (B. cereus), Pseudomonas aeruginosa MTCC 424 (P. aeruginosa), Proteus mirabilis MTCC 3310 (P. mirabilis) and Escherichia coli MTCC 40 (E. coli). The synthesized ZnO NPs have shown antibacterial efficacy against both Gram-positive and Gram-negative pathogens. Synergistic effects of ZnO NPs and streptomycin showed increased efficacy as indicated by the increased zone of clearance in comparison to their individual effects (either ZnO NPs or streptomycin). Overall, the results elucidated a rapid, cost-effective, environmentally friendly and convenient method for ZnO NPs synthesis, which could be used as a potential antimicrobial agent against drug resistant microbes. Frontiers Media S.A. 2018-09-03 /pmc/articles/PMC6129596/ /pubmed/30233518 http://dx.doi.org/10.3389/fmicb.2018.02030 Text en Copyright © 2018 Gupta, Tomar, Kaushik, Mishra and Sharma. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Microbiology Gupta, Monika Tomar, Rajesh S. Kaushik, Shuchi Mishra, Raghvendra K. Sharma, Divakar Effective Antimicrobial Activity of Green ZnO Nano Particles of Catharanthus roseus |
title | Effective Antimicrobial Activity of Green ZnO Nano Particles of Catharanthus roseus |
title_full | Effective Antimicrobial Activity of Green ZnO Nano Particles of Catharanthus roseus |
title_fullStr | Effective Antimicrobial Activity of Green ZnO Nano Particles of Catharanthus roseus |
title_full_unstemmed | Effective Antimicrobial Activity of Green ZnO Nano Particles of Catharanthus roseus |
title_short | Effective Antimicrobial Activity of Green ZnO Nano Particles of Catharanthus roseus |
title_sort | effective antimicrobial activity of green zno nano particles of catharanthus roseus |
topic | Microbiology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6129596/ https://www.ncbi.nlm.nih.gov/pubmed/30233518 http://dx.doi.org/10.3389/fmicb.2018.02030 |
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