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Effect of aerobic training on baseline expression of signaling and respiratory proteins in human skeletal muscle

Most studies examining the molecular mechanisms underlying adaptation of human skeletal muscles to aerobic exercise focused on the response to acute exercise. Here, we examined the effect of a 2‐month aerobic training program on baseline parameters in human muscle. Ten untrained males performed a on...

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Autores principales: Popov, Daniil V., Lysenko, Evgeny A., Bokov, Roman O., Volodina, Maria A., Kurochkina, Nadia S., Makhnovskii, Pavel A., Vyssokikh, Mikhail Y., Vinogradova, Olga L.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6129775/
https://www.ncbi.nlm.nih.gov/pubmed/30198217
http://dx.doi.org/10.14814/phy2.13868
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author Popov, Daniil V.
Lysenko, Evgeny A.
Bokov, Roman O.
Volodina, Maria A.
Kurochkina, Nadia S.
Makhnovskii, Pavel A.
Vyssokikh, Mikhail Y.
Vinogradova, Olga L.
author_facet Popov, Daniil V.
Lysenko, Evgeny A.
Bokov, Roman O.
Volodina, Maria A.
Kurochkina, Nadia S.
Makhnovskii, Pavel A.
Vyssokikh, Mikhail Y.
Vinogradova, Olga L.
author_sort Popov, Daniil V.
collection PubMed
description Most studies examining the molecular mechanisms underlying adaptation of human skeletal muscles to aerobic exercise focused on the response to acute exercise. Here, we examined the effect of a 2‐month aerobic training program on baseline parameters in human muscle. Ten untrained males performed a one‐legged knee extension exercise for 1 h with the same relative intensity before and after a 2‐month aerobic training program. Biopsy samples were taken from vastus lateralis muscle at rest before and after the 2 month training program (baseline samples). Additionally, biopsy samples were taken from the exercised leg 1 and 4 h after the one‐legged continuous knee extension exercise. Aerobic training decreases baseline phosphorylation of FOXO1(Ser256), increases that of CaMKII(T) (hr286), CREB1(Ser133), increases baseline expression of mitochondrial proteins in respiratory complexes I–V, and some regulators of mitochondrial biogenesis (TFAM, NR4A3, and CRTC2). An increase in the baseline content of these proteins was not associated with a change in baseline expression of their genes. The increase in the baseline content of regulators of mitochondrial biogenesis (TFAM and NR4A3) was associated with a transient increase in transcription after acute exercise. Contrariwise, the increase in the baseline content of respiratory proteins does not seem to be regulated at the transcriptional level; rather, it is associated with other mechanisms. Adaptation of human skeletal muscle to regular aerobic exercise is associated not only with transient molecular responses to exercise, but also with changes in baseline phosphorylation and expression of regulatory proteins.
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spelling pubmed-61297752018-09-13 Effect of aerobic training on baseline expression of signaling and respiratory proteins in human skeletal muscle Popov, Daniil V. Lysenko, Evgeny A. Bokov, Roman O. Volodina, Maria A. Kurochkina, Nadia S. Makhnovskii, Pavel A. Vyssokikh, Mikhail Y. Vinogradova, Olga L. Physiol Rep Original Research Most studies examining the molecular mechanisms underlying adaptation of human skeletal muscles to aerobic exercise focused on the response to acute exercise. Here, we examined the effect of a 2‐month aerobic training program on baseline parameters in human muscle. Ten untrained males performed a one‐legged knee extension exercise for 1 h with the same relative intensity before and after a 2‐month aerobic training program. Biopsy samples were taken from vastus lateralis muscle at rest before and after the 2 month training program (baseline samples). Additionally, biopsy samples were taken from the exercised leg 1 and 4 h after the one‐legged continuous knee extension exercise. Aerobic training decreases baseline phosphorylation of FOXO1(Ser256), increases that of CaMKII(T) (hr286), CREB1(Ser133), increases baseline expression of mitochondrial proteins in respiratory complexes I–V, and some regulators of mitochondrial biogenesis (TFAM, NR4A3, and CRTC2). An increase in the baseline content of these proteins was not associated with a change in baseline expression of their genes. The increase in the baseline content of regulators of mitochondrial biogenesis (TFAM and NR4A3) was associated with a transient increase in transcription after acute exercise. Contrariwise, the increase in the baseline content of respiratory proteins does not seem to be regulated at the transcriptional level; rather, it is associated with other mechanisms. Adaptation of human skeletal muscle to regular aerobic exercise is associated not only with transient molecular responses to exercise, but also with changes in baseline phosphorylation and expression of regulatory proteins. John Wiley and Sons Inc. 2018-09-10 /pmc/articles/PMC6129775/ /pubmed/30198217 http://dx.doi.org/10.14814/phy2.13868 Text en © 2018 The Authors. Physiological Reports published by Wiley Periodicals, Inc. on behalf of The Physiological Society and the American Physiological Society. This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Research
Popov, Daniil V.
Lysenko, Evgeny A.
Bokov, Roman O.
Volodina, Maria A.
Kurochkina, Nadia S.
Makhnovskii, Pavel A.
Vyssokikh, Mikhail Y.
Vinogradova, Olga L.
Effect of aerobic training on baseline expression of signaling and respiratory proteins in human skeletal muscle
title Effect of aerobic training on baseline expression of signaling and respiratory proteins in human skeletal muscle
title_full Effect of aerobic training on baseline expression of signaling and respiratory proteins in human skeletal muscle
title_fullStr Effect of aerobic training on baseline expression of signaling and respiratory proteins in human skeletal muscle
title_full_unstemmed Effect of aerobic training on baseline expression of signaling and respiratory proteins in human skeletal muscle
title_short Effect of aerobic training on baseline expression of signaling and respiratory proteins in human skeletal muscle
title_sort effect of aerobic training on baseline expression of signaling and respiratory proteins in human skeletal muscle
topic Original Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6129775/
https://www.ncbi.nlm.nih.gov/pubmed/30198217
http://dx.doi.org/10.14814/phy2.13868
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