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Three-dimensional tumor cell cultures employed in virotherapy research

Oncolytic virotherapy constitutes an upcoming alternative treatment option for a broad spectrum of cancer entities. However, despite great research efforts, there is still only a single US Food and Drug Administration/European Medicines Agency-approved oncolytic virus available for clinical use. One...

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Detalles Bibliográficos
Autores principales: Kloker, Linus D, Yurttas, Can, Lauer, Ulrich M
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Dove Medical Press 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6130269/
https://www.ncbi.nlm.nih.gov/pubmed/30234074
http://dx.doi.org/10.2147/OV.S165479
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author Kloker, Linus D
Yurttas, Can
Lauer, Ulrich M
author_facet Kloker, Linus D
Yurttas, Can
Lauer, Ulrich M
author_sort Kloker, Linus D
collection PubMed
description Oncolytic virotherapy constitutes an upcoming alternative treatment option for a broad spectrum of cancer entities. However, despite great research efforts, there is still only a single US Food and Drug Administration/European Medicines Agency-approved oncolytic virus available for clinical use. One reason for that is the gap between promising preclinical data and limited clinical success. Since oncolytic viruses are biological agents, they might require more realistic in vitro tumor models than common monolayer tumor cell cultures to provide meaningful predictive preclinical evaluation results. For more realistic invitro tumor models, three-dimensional tumor cell-culture systems can be employed in preclinical virotherapy research. This review provides an overview of spheroid and hydrogel tumor cell cultures, organotypic tumor-tissue slices, organotypic raft cultures, and tumor organoids utilized in the context of oncolytic virotherapy. Furthermore, we also discuss advantages, disadvantages, techniques, and difficulties of these three-dimensional tumor cell-culture systems when applied specifically in virotherapy research.
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spelling pubmed-61302692018-09-19 Three-dimensional tumor cell cultures employed in virotherapy research Kloker, Linus D Yurttas, Can Lauer, Ulrich M Oncolytic Virother Review Oncolytic virotherapy constitutes an upcoming alternative treatment option for a broad spectrum of cancer entities. However, despite great research efforts, there is still only a single US Food and Drug Administration/European Medicines Agency-approved oncolytic virus available for clinical use. One reason for that is the gap between promising preclinical data and limited clinical success. Since oncolytic viruses are biological agents, they might require more realistic in vitro tumor models than common monolayer tumor cell cultures to provide meaningful predictive preclinical evaluation results. For more realistic invitro tumor models, three-dimensional tumor cell-culture systems can be employed in preclinical virotherapy research. This review provides an overview of spheroid and hydrogel tumor cell cultures, organotypic tumor-tissue slices, organotypic raft cultures, and tumor organoids utilized in the context of oncolytic virotherapy. Furthermore, we also discuss advantages, disadvantages, techniques, and difficulties of these three-dimensional tumor cell-culture systems when applied specifically in virotherapy research. Dove Medical Press 2018-09-05 /pmc/articles/PMC6130269/ /pubmed/30234074 http://dx.doi.org/10.2147/OV.S165479 Text en © 2018 Kloker et al. This work is published and licensed by Dove Medical Press Limited The full terms of this license are available at https://www.dovepress.com/terms.php and incorporate the Creative Commons Attribution – Non Commercial (unported, v3.0) License (http://creativecommons.org/licenses/by-nc/3.0/). By accessing the work you hereby accept the Terms. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed.
spellingShingle Review
Kloker, Linus D
Yurttas, Can
Lauer, Ulrich M
Three-dimensional tumor cell cultures employed in virotherapy research
title Three-dimensional tumor cell cultures employed in virotherapy research
title_full Three-dimensional tumor cell cultures employed in virotherapy research
title_fullStr Three-dimensional tumor cell cultures employed in virotherapy research
title_full_unstemmed Three-dimensional tumor cell cultures employed in virotherapy research
title_short Three-dimensional tumor cell cultures employed in virotherapy research
title_sort three-dimensional tumor cell cultures employed in virotherapy research
topic Review
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6130269/
https://www.ncbi.nlm.nih.gov/pubmed/30234074
http://dx.doi.org/10.2147/OV.S165479
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