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IL-17 and colorectal cancer risk in the Middle East: gene polymorphisms and expression

BACKGROUND: IL-17 expressed by Th17 cells play a crucial role in tissue inflammation by induction of proinflammatory and neutrophil mobilizing cytokines, and IL-17 polymorphisms are associated with colorectal cancer (CRC). OBJECTIVE: We investigated the expression of IL-17 and the association of IL-...

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Autores principales: Al Obeed, Omar A, Vaali-Mohamed, Mansoor-Ali, Alkhayal, Khayal A, Bin Traiki, Thamer A, Zubaidi, Ahmad M, Arafah, Maha, Harris, Robert A, Khan, Zahid, Abdulla, Maha-Hamadien
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Dove Medical Press 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6130533/
https://www.ncbi.nlm.nih.gov/pubmed/30233234
http://dx.doi.org/10.2147/CMAR.S161248
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author Al Obeed, Omar A
Vaali-Mohamed, Mansoor-Ali
Alkhayal, Khayal A
Bin Traiki, Thamer A
Zubaidi, Ahmad M
Arafah, Maha
Harris, Robert A
Khan, Zahid
Abdulla, Maha-Hamadien
author_facet Al Obeed, Omar A
Vaali-Mohamed, Mansoor-Ali
Alkhayal, Khayal A
Bin Traiki, Thamer A
Zubaidi, Ahmad M
Arafah, Maha
Harris, Robert A
Khan, Zahid
Abdulla, Maha-Hamadien
author_sort Al Obeed, Omar A
collection PubMed
description BACKGROUND: IL-17 expressed by Th17 cells play a crucial role in tissue inflammation by induction of proinflammatory and neutrophil mobilizing cytokines, and IL-17 polymorphisms are associated with colorectal cancer (CRC). OBJECTIVE: We investigated the expression of IL-17 and the association of IL-17 gene polymorphisms with CRC susceptibility in a Middle East population. MATERIALS AND METHODS: The study included 117 diagnosed CRC patients and 100 age- and gender-matched healthy controls. IL-17A rs2275913 (G197A) and IL-17F rs763780 (T7488C) single nucleotide polymorphisms, mRNA, and protein levels of IL-17A were assessed. RESULTS: We observed significant association between rs2275913 in IL-17A and susceptibility to CRC (p = 0.016228). The AG and AA genotypes conferred 2-fold and 2.8-fold, respectively, higher risk of developing CRC compared with individuals having GG genotype. Stratification of the data based on gender and age revealed very strong association of CRC with IL17A rs2275913 only in males and “AG” genotype in patients ≤57 years of age at the time of disease diagnosis. The rs763780 in IL-17F was not linked with CRCs in our cohort. Furthermore, IL-17A mRNA expression in CRCs was significantly elevated compared to adjacent normal tissues, particularly in early stages of disease (p = 0.0005). Strong immunoreactivity to IL-17A protein was observed in 70% of early stage relative to 30% of late-stage tumors. CONCLUSION: The IL-17A G197A variant may be utilized as a genetic screening marker in assessing CRC risk, and its expression can be used as a biomarker for early detection of CRC in the Saudi population.
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spelling pubmed-61305332018-09-19 IL-17 and colorectal cancer risk in the Middle East: gene polymorphisms and expression Al Obeed, Omar A Vaali-Mohamed, Mansoor-Ali Alkhayal, Khayal A Bin Traiki, Thamer A Zubaidi, Ahmad M Arafah, Maha Harris, Robert A Khan, Zahid Abdulla, Maha-Hamadien Cancer Manag Res Original Research BACKGROUND: IL-17 expressed by Th17 cells play a crucial role in tissue inflammation by induction of proinflammatory and neutrophil mobilizing cytokines, and IL-17 polymorphisms are associated with colorectal cancer (CRC). OBJECTIVE: We investigated the expression of IL-17 and the association of IL-17 gene polymorphisms with CRC susceptibility in a Middle East population. MATERIALS AND METHODS: The study included 117 diagnosed CRC patients and 100 age- and gender-matched healthy controls. IL-17A rs2275913 (G197A) and IL-17F rs763780 (T7488C) single nucleotide polymorphisms, mRNA, and protein levels of IL-17A were assessed. RESULTS: We observed significant association between rs2275913 in IL-17A and susceptibility to CRC (p = 0.016228). The AG and AA genotypes conferred 2-fold and 2.8-fold, respectively, higher risk of developing CRC compared with individuals having GG genotype. Stratification of the data based on gender and age revealed very strong association of CRC with IL17A rs2275913 only in males and “AG” genotype in patients ≤57 years of age at the time of disease diagnosis. The rs763780 in IL-17F was not linked with CRCs in our cohort. Furthermore, IL-17A mRNA expression in CRCs was significantly elevated compared to adjacent normal tissues, particularly in early stages of disease (p = 0.0005). Strong immunoreactivity to IL-17A protein was observed in 70% of early stage relative to 30% of late-stage tumors. CONCLUSION: The IL-17A G197A variant may be utilized as a genetic screening marker in assessing CRC risk, and its expression can be used as a biomarker for early detection of CRC in the Saudi population. Dove Medical Press 2018-09-05 /pmc/articles/PMC6130533/ /pubmed/30233234 http://dx.doi.org/10.2147/CMAR.S161248 Text en © 2018 Al Obeed et al. This work is published and licensed by Dove Medical Press Limited The full terms of this license are available at https://www.dovepress.com/terms.php and incorporate the Creative Commons Attribution – Non Commercial (unported, v3.0) License (http://creativecommons.org/licenses/by-nc/3.0/). By accessing the work you hereby accept the Terms. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed.
spellingShingle Original Research
Al Obeed, Omar A
Vaali-Mohamed, Mansoor-Ali
Alkhayal, Khayal A
Bin Traiki, Thamer A
Zubaidi, Ahmad M
Arafah, Maha
Harris, Robert A
Khan, Zahid
Abdulla, Maha-Hamadien
IL-17 and colorectal cancer risk in the Middle East: gene polymorphisms and expression
title IL-17 and colorectal cancer risk in the Middle East: gene polymorphisms and expression
title_full IL-17 and colorectal cancer risk in the Middle East: gene polymorphisms and expression
title_fullStr IL-17 and colorectal cancer risk in the Middle East: gene polymorphisms and expression
title_full_unstemmed IL-17 and colorectal cancer risk in the Middle East: gene polymorphisms and expression
title_short IL-17 and colorectal cancer risk in the Middle East: gene polymorphisms and expression
title_sort il-17 and colorectal cancer risk in the middle east: gene polymorphisms and expression
topic Original Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6130533/
https://www.ncbi.nlm.nih.gov/pubmed/30233234
http://dx.doi.org/10.2147/CMAR.S161248
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