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Appraisal of anti-arthritic and nephroprotective potential of Cuscuta reflexa
Context: Cuscuta reflexa Roxb. (Cuscutaceae) has been used traditionally for treating sore knees and kidney problems, but its efficacy has not been scientifically examined in treating arthritis and nephrotoxicity. Objective: Present study determines antiarthritic and nephroprotective potential of th...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Taylor & Francis
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6130659/ https://www.ncbi.nlm.nih.gov/pubmed/28103731 http://dx.doi.org/10.1080/13880209.2017.1280513 |
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author | Alamgeer, Niazi, Samia Gul Uttra, Ambreen Malik Qaiser, Muhammad Naeem Ahsan, Haseeb |
author_facet | Alamgeer, Niazi, Samia Gul Uttra, Ambreen Malik Qaiser, Muhammad Naeem Ahsan, Haseeb |
author_sort | Alamgeer, |
collection | PubMed |
description | Context: Cuscuta reflexa Roxb. (Cuscutaceae) has been used traditionally for treating sore knees and kidney problems, but its efficacy has not been scientifically examined in treating arthritis and nephrotoxicity. Objective: Present study determines antiarthritic and nephroprotective potential of the aqueous methanolic extract of Cuscuta reflexa (AMECR). Materials and methods: Antiarthritic activity of Cuscuta reflexa in formaldehyde and turpentine oil-induced rat arthritis models was appraised at 200, 400 and 600 mg/kg doses for 10 days and 6 h period, respectively, and in vitro protein denaturation (bovine serum albumin, egg albumin) inhibition was studied at 25–800 μg/mL concentration. The nephroprotective effect involved gentamicin-induced nephrotoxicity in rats at 200, 400 and 600 mg/kg doses. Results: Plant extract at 600 mg/kg significantly reduced paw oedema and joint swelling with maximal inhibition of 71.22% at the 6th hour for turpentine oil and 76.74% on 10th day for formaldehyde. Likewise, in vitro results corroborated significant concentration-dependent increase in percentage protection at 800 μg/mL against both bovine serum albumin (89.30%) and egg albumin (93.51%) denaturation. Similarly, 600 mg/kg dose showed maximum nephroprotection by reducing serum urea (41.400 ± 0.510 mg/dL), uric acid (0.740 ± 0.032 mg/dL), blood urea nitrogen (18.370 ± 0.328), creatinine (3.267 ± 0.076) and minimizing kidney weight gain (0.586 ± 0.005) and histopathological alterations on 8th day. Furthermore, phytochemical and HPLC analysis revealed the presence of important phytoconstituents. Discussion and conclusions: These results suggest that AMECR provides protection against arthritis and nephrotoxicity that might be due to the existence of phytoconstituents, thus supporting folkloric claim. |
format | Online Article Text |
id | pubmed-6130659 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | Taylor & Francis |
record_format | MEDLINE/PubMed |
spelling | pubmed-61306592018-09-27 Appraisal of anti-arthritic and nephroprotective potential of Cuscuta reflexa Alamgeer, Niazi, Samia Gul Uttra, Ambreen Malik Qaiser, Muhammad Naeem Ahsan, Haseeb Pharm Biol Research Article Context: Cuscuta reflexa Roxb. (Cuscutaceae) has been used traditionally for treating sore knees and kidney problems, but its efficacy has not been scientifically examined in treating arthritis and nephrotoxicity. Objective: Present study determines antiarthritic and nephroprotective potential of the aqueous methanolic extract of Cuscuta reflexa (AMECR). Materials and methods: Antiarthritic activity of Cuscuta reflexa in formaldehyde and turpentine oil-induced rat arthritis models was appraised at 200, 400 and 600 mg/kg doses for 10 days and 6 h period, respectively, and in vitro protein denaturation (bovine serum albumin, egg albumin) inhibition was studied at 25–800 μg/mL concentration. The nephroprotective effect involved gentamicin-induced nephrotoxicity in rats at 200, 400 and 600 mg/kg doses. Results: Plant extract at 600 mg/kg significantly reduced paw oedema and joint swelling with maximal inhibition of 71.22% at the 6th hour for turpentine oil and 76.74% on 10th day for formaldehyde. Likewise, in vitro results corroborated significant concentration-dependent increase in percentage protection at 800 μg/mL against both bovine serum albumin (89.30%) and egg albumin (93.51%) denaturation. Similarly, 600 mg/kg dose showed maximum nephroprotection by reducing serum urea (41.400 ± 0.510 mg/dL), uric acid (0.740 ± 0.032 mg/dL), blood urea nitrogen (18.370 ± 0.328), creatinine (3.267 ± 0.076) and minimizing kidney weight gain (0.586 ± 0.005) and histopathological alterations on 8th day. Furthermore, phytochemical and HPLC analysis revealed the presence of important phytoconstituents. Discussion and conclusions: These results suggest that AMECR provides protection against arthritis and nephrotoxicity that might be due to the existence of phytoconstituents, thus supporting folkloric claim. Taylor & Francis 2017-01-19 /pmc/articles/PMC6130659/ /pubmed/28103731 http://dx.doi.org/10.1080/13880209.2017.1280513 Text en © 2017 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group. http://creativecommons.org/licenses/by/4.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Alamgeer, Niazi, Samia Gul Uttra, Ambreen Malik Qaiser, Muhammad Naeem Ahsan, Haseeb Appraisal of anti-arthritic and nephroprotective potential of Cuscuta reflexa |
title | Appraisal of anti-arthritic and nephroprotective potential of Cuscuta reflexa
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title_full | Appraisal of anti-arthritic and nephroprotective potential of Cuscuta reflexa
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title_fullStr | Appraisal of anti-arthritic and nephroprotective potential of Cuscuta reflexa
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title_full_unstemmed | Appraisal of anti-arthritic and nephroprotective potential of Cuscuta reflexa
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title_short | Appraisal of anti-arthritic and nephroprotective potential of Cuscuta reflexa
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title_sort | appraisal of anti-arthritic and nephroprotective potential of cuscuta reflexa |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6130659/ https://www.ncbi.nlm.nih.gov/pubmed/28103731 http://dx.doi.org/10.1080/13880209.2017.1280513 |
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