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Phytochemical, antioxidant and protective effect of cactus cladodes extract against lithium-induced liver injury in rats

Context: Opuntia ficus-indica (L.) Mill. (Castaceae) (cactus) is used in Tunisian medicine for the treatment of various diseases. Objective: This study determines phytochemical composition of cactus cladode extract (CCE). It also investigates antioxidant activity and hepatoprotective potential of CC...

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Autores principales: Ben Saad, Anouar, Dalel, Brahmi, Rjeibi, Ilhem, Smida, Amani, Ncib, Sana, Zouari, Nacim, Zourgui, Lazhar
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Taylor & Francis 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6130665/
https://www.ncbi.nlm.nih.gov/pubmed/27951739
http://dx.doi.org/10.1080/13880209.2016.1255976
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author Ben Saad, Anouar
Dalel, Brahmi
Rjeibi, Ilhem
Smida, Amani
Ncib, Sana
Zouari, Nacim
Zourgui, Lazhar
author_facet Ben Saad, Anouar
Dalel, Brahmi
Rjeibi, Ilhem
Smida, Amani
Ncib, Sana
Zouari, Nacim
Zourgui, Lazhar
author_sort Ben Saad, Anouar
collection PubMed
description Context: Opuntia ficus-indica (L.) Mill. (Castaceae) (cactus) is used in Tunisian medicine for the treatment of various diseases. Objective: This study determines phytochemical composition of cactus cladode extract (CCE). It also investigates antioxidant activity and hepatoprotective potential of CCE against lithium carbonate (Li(2)CO(3))-induced liver injury in rats. Materials and methods: Twenty-four Wistar male rats were divided into four groups of six each: a control group given distilled water (0.5 mL/100 g b.w.; i.p.), a group injected with Li(2)CO(3) (25 mg/kg b.w.; i.p.; corresponding to 30% of the LD(50)) twice daily for 30 days, a group receiving only CCE at 100 mg/kg of b.w. for 60 days and then injected with distilled water during the last 30 days of CCE treatment, and a group receiving CCE and then injected with Li(2)CO(3) during the last 30 days of CCE treatment. The bioactive components containing the CCE were identified using chemical assays. Results: Treatment with Li(2)CO(3) caused a significant change of some haematological parameters including red blood cells (RBC), white blood cells (WBC), haemoglobin content (Hb), haematocrit (Ht) and mean corpuscular volume (VCM) compared to the control group. Moreover, significant increases in the levels of glucose, cholesterol, triglycerides and of aspartate aminotransferase (AST), alanine aminotransferase (ALT), alkaline phosphatase (ALP) and lactate dehydrogenase (LDH) activities were observed in the blood of Li(2)CO(3)-treated rats. Furthermore, exposure to Li(2)CO(3) significantly increased the LPO level and decreased superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GPx) activities in the hepatic tissues. Conclusion: CCE possesses a significant hepatoprotective effect.
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spelling pubmed-61306652018-09-27 Phytochemical, antioxidant and protective effect of cactus cladodes extract against lithium-induced liver injury in rats Ben Saad, Anouar Dalel, Brahmi Rjeibi, Ilhem Smida, Amani Ncib, Sana Zouari, Nacim Zourgui, Lazhar Pharm Biol Research Article Context: Opuntia ficus-indica (L.) Mill. (Castaceae) (cactus) is used in Tunisian medicine for the treatment of various diseases. Objective: This study determines phytochemical composition of cactus cladode extract (CCE). It also investigates antioxidant activity and hepatoprotective potential of CCE against lithium carbonate (Li(2)CO(3))-induced liver injury in rats. Materials and methods: Twenty-four Wistar male rats were divided into four groups of six each: a control group given distilled water (0.5 mL/100 g b.w.; i.p.), a group injected with Li(2)CO(3) (25 mg/kg b.w.; i.p.; corresponding to 30% of the LD(50)) twice daily for 30 days, a group receiving only CCE at 100 mg/kg of b.w. for 60 days and then injected with distilled water during the last 30 days of CCE treatment, and a group receiving CCE and then injected with Li(2)CO(3) during the last 30 days of CCE treatment. The bioactive components containing the CCE were identified using chemical assays. Results: Treatment with Li(2)CO(3) caused a significant change of some haematological parameters including red blood cells (RBC), white blood cells (WBC), haemoglobin content (Hb), haematocrit (Ht) and mean corpuscular volume (VCM) compared to the control group. Moreover, significant increases in the levels of glucose, cholesterol, triglycerides and of aspartate aminotransferase (AST), alanine aminotransferase (ALT), alkaline phosphatase (ALP) and lactate dehydrogenase (LDH) activities were observed in the blood of Li(2)CO(3)-treated rats. Furthermore, exposure to Li(2)CO(3) significantly increased the LPO level and decreased superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GPx) activities in the hepatic tissues. Conclusion: CCE possesses a significant hepatoprotective effect. Taylor & Francis 2016-12-12 /pmc/articles/PMC6130665/ /pubmed/27951739 http://dx.doi.org/10.1080/13880209.2016.1255976 Text en © 2016 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group. http://creativecommons.org/licenses/by/4.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Ben Saad, Anouar
Dalel, Brahmi
Rjeibi, Ilhem
Smida, Amani
Ncib, Sana
Zouari, Nacim
Zourgui, Lazhar
Phytochemical, antioxidant and protective effect of cactus cladodes extract against lithium-induced liver injury in rats
title Phytochemical, antioxidant and protective effect of cactus cladodes extract against lithium-induced liver injury in rats
title_full Phytochemical, antioxidant and protective effect of cactus cladodes extract against lithium-induced liver injury in rats
title_fullStr Phytochemical, antioxidant and protective effect of cactus cladodes extract against lithium-induced liver injury in rats
title_full_unstemmed Phytochemical, antioxidant and protective effect of cactus cladodes extract against lithium-induced liver injury in rats
title_short Phytochemical, antioxidant and protective effect of cactus cladodes extract against lithium-induced liver injury in rats
title_sort phytochemical, antioxidant and protective effect of cactus cladodes extract against lithium-induced liver injury in rats
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6130665/
https://www.ncbi.nlm.nih.gov/pubmed/27951739
http://dx.doi.org/10.1080/13880209.2016.1255976
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