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Isolation and identification of compounds from Kalanchoe pinnata having human alphaherpesvirus and vaccinia virus antiviral activity
Context:Kalanchoe pinnata (Lam.) Pers. (Crassulaceae) is a succulent plant that is known for its traditional antivirus and antibacterial usage. Objective: This work examines two compounds identified from the K. pinnata plant for their antivirus activity against human alphaherpesvirus (HHV) 1 and 2 a...
Autores principales: | , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Taylor & Francis
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6130675/ https://www.ncbi.nlm.nih.gov/pubmed/28395583 http://dx.doi.org/10.1080/13880209.2017.1310907 |
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author | Cryer, Matthew Lane, Kyle Greer, Mary Cates, Rex Burt, Scott Andrus, Merritt Zou, Jiping Rogers, Paul Hansen, Marc D. H. Burgado, Jillybeth Satheshkumar, Panayampalli Subbian Day, Craig W. Smee, Donald F. Johnson, F. Brent |
author_facet | Cryer, Matthew Lane, Kyle Greer, Mary Cates, Rex Burt, Scott Andrus, Merritt Zou, Jiping Rogers, Paul Hansen, Marc D. H. Burgado, Jillybeth Satheshkumar, Panayampalli Subbian Day, Craig W. Smee, Donald F. Johnson, F. Brent |
author_sort | Cryer, Matthew |
collection | PubMed |
description | Context:Kalanchoe pinnata (Lam.) Pers. (Crassulaceae) is a succulent plant that is known for its traditional antivirus and antibacterial usage. Objective: This work examines two compounds identified from the K. pinnata plant for their antivirus activity against human alphaherpesvirus (HHV) 1 and 2 and vaccinia virus (VACV). Materials and methods: Compounds KPB-100 and KPB-200 were isolated using HPLC and were identified using NMR and MS. Both compounds were tested in plaque reduction assay of HHV-2 wild type (WT) and VACV. Both compounds were then tested in virus spread inhibition and virus yield reduction (VYR) assays of VACV. KPB-100 was further tested in viral cytopathic effect (CPE) inhibition assay of HHV-2 TK-mutant and VYR assay of HHV-1 WT. Results: KPB-100 and KPB-200 inhibited HHV-2 at IC(50) values of 2.5 and 2.9 μg/mL, respectively, and VACV at IC(50) values of 3.1 and 7.4 μg/mL, respectively, in plaque reduction assays. In virus spread inhibition assay of VACV KPB-100 and KPB-200 yielded IC(50) values of 1.63 and 13.2 μg/mL, respectively, and KPB-100 showed a nearly 2-log reduction in virus in VYR assay of VACV at 20 μg/mL. Finally, KPB-100 inhibited HHV-2 TK- at an IC(50) value of 4.5 μg/mL in CPE inhibition assay and HHV-1 at an IC(90) of 3.0 μg/mL in VYR assay. Discussion and conclusion: Both compounds are promising targets for synthetic optimization and in vivo study. KPB-100 in particular showed strong inhibition of all viruses tested. |
format | Online Article Text |
id | pubmed-6130675 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | Taylor & Francis |
record_format | MEDLINE/PubMed |
spelling | pubmed-61306752018-09-27 Isolation and identification of compounds from Kalanchoe pinnata having human alphaherpesvirus and vaccinia virus antiviral activity Cryer, Matthew Lane, Kyle Greer, Mary Cates, Rex Burt, Scott Andrus, Merritt Zou, Jiping Rogers, Paul Hansen, Marc D. H. Burgado, Jillybeth Satheshkumar, Panayampalli Subbian Day, Craig W. Smee, Donald F. Johnson, F. Brent Pharm Biol Research Article Context:Kalanchoe pinnata (Lam.) Pers. (Crassulaceae) is a succulent plant that is known for its traditional antivirus and antibacterial usage. Objective: This work examines two compounds identified from the K. pinnata plant for their antivirus activity against human alphaherpesvirus (HHV) 1 and 2 and vaccinia virus (VACV). Materials and methods: Compounds KPB-100 and KPB-200 were isolated using HPLC and were identified using NMR and MS. Both compounds were tested in plaque reduction assay of HHV-2 wild type (WT) and VACV. Both compounds were then tested in virus spread inhibition and virus yield reduction (VYR) assays of VACV. KPB-100 was further tested in viral cytopathic effect (CPE) inhibition assay of HHV-2 TK-mutant and VYR assay of HHV-1 WT. Results: KPB-100 and KPB-200 inhibited HHV-2 at IC(50) values of 2.5 and 2.9 μg/mL, respectively, and VACV at IC(50) values of 3.1 and 7.4 μg/mL, respectively, in plaque reduction assays. In virus spread inhibition assay of VACV KPB-100 and KPB-200 yielded IC(50) values of 1.63 and 13.2 μg/mL, respectively, and KPB-100 showed a nearly 2-log reduction in virus in VYR assay of VACV at 20 μg/mL. Finally, KPB-100 inhibited HHV-2 TK- at an IC(50) value of 4.5 μg/mL in CPE inhibition assay and HHV-1 at an IC(90) of 3.0 μg/mL in VYR assay. Discussion and conclusion: Both compounds are promising targets for synthetic optimization and in vivo study. KPB-100 in particular showed strong inhibition of all viruses tested. Taylor & Francis 2017-04-11 /pmc/articles/PMC6130675/ /pubmed/28395583 http://dx.doi.org/10.1080/13880209.2017.1310907 Text en © 2017 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group. http://creativecommons.org/Licenses/by/4.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/Licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Cryer, Matthew Lane, Kyle Greer, Mary Cates, Rex Burt, Scott Andrus, Merritt Zou, Jiping Rogers, Paul Hansen, Marc D. H. Burgado, Jillybeth Satheshkumar, Panayampalli Subbian Day, Craig W. Smee, Donald F. Johnson, F. Brent Isolation and identification of compounds from Kalanchoe pinnata having human alphaherpesvirus and vaccinia virus antiviral activity |
title | Isolation and identification of compounds from Kalanchoe pinnata having human alphaherpesvirus and vaccinia virus antiviral activity |
title_full | Isolation and identification of compounds from Kalanchoe pinnata having human alphaherpesvirus and vaccinia virus antiviral activity |
title_fullStr | Isolation and identification of compounds from Kalanchoe pinnata having human alphaherpesvirus and vaccinia virus antiviral activity |
title_full_unstemmed | Isolation and identification of compounds from Kalanchoe pinnata having human alphaherpesvirus and vaccinia virus antiviral activity |
title_short | Isolation and identification of compounds from Kalanchoe pinnata having human alphaherpesvirus and vaccinia virus antiviral activity |
title_sort | isolation and identification of compounds from kalanchoe pinnata having human alphaherpesvirus and vaccinia virus antiviral activity |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6130675/ https://www.ncbi.nlm.nih.gov/pubmed/28395583 http://dx.doi.org/10.1080/13880209.2017.1310907 |
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