Antioxidant, anti-inflammatory and anti-septic potential of phenolic acids and flavonoid fractions isolated from Lolium multiflorum

Context: Interest has recently renewed in using Lolium multiflorum Lam. (Poaceae) (called Italian ryegrass; IRG) silage as an antioxidant and anti-inflammatory diet. Objective: This study investigated the antioxidant, anti-inflammatory and anti-septic potential of IRG silage and identified the prima...

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Autores principales: Choi, Ki-Choon, Son, Young-Ok, Hwang, Jung-Min, Kim, Beom-Tae, Chae, Minseon, Lee, Jeong-Chae
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Taylor & Francis 2016
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Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6130696/
https://www.ncbi.nlm.nih.gov/pubmed/27937124
http://dx.doi.org/10.1080/13880209.2016.1266673
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author Choi, Ki-Choon
Son, Young-Ok
Hwang, Jung-Min
Kim, Beom-Tae
Chae, Minseon
Lee, Jeong-Chae
author_facet Choi, Ki-Choon
Son, Young-Ok
Hwang, Jung-Min
Kim, Beom-Tae
Chae, Minseon
Lee, Jeong-Chae
author_sort Choi, Ki-Choon
collection PubMed
description Context: Interest has recently renewed in using Lolium multiflorum Lam. (Poaceae) (called Italian ryegrass; IRG) silage as an antioxidant and anti-inflammatory diet. Objective: This study investigated the antioxidant, anti-inflammatory and anti-septic potential of IRG silage and identified the primary components in IRG active fractions. Materials and methods: Total 16 fractions were separated from the chloroform-soluble extract of IRG aerial part using Sephadex LH-20 column before HPLC analysis. Antioxidant and anti-inflammatory activities of the fractions at doses of 0–100 μg/mL were investigated using various cell-free and cell-mediated assay systems. To explore anti-septic effect of IRG fractions, female ICR and BALB/c mice orally received 40 mg/kg of phenolic acid and flavonoid-rich active fractions F(7) and F(8) every other day for 10 days, respectively, followed by LPS challenge. Results: The active fractions showed greater antioxidant and anti-inflammatory potential compared with other fractions. IC(50) values of F(7) and F(8) to reduce LPS-stimulated NO and TNF-α production were around 15 and 30 μg/mL, respectively. Comparison of retention times with authentic compounds through HPLC analysis revealed the presence of caffeic acid, ferulic acid, myricetin and kaempferol in the fractions as primary components. These fractions inhibited LPS-stimulated MAPK and NF-κB activation. Supplementation with F(7) or F(8) improved the survival rates of mice to 70 and 60%, respectively, in LPS-injected mice and reduced near completely serum TNF-α and IL-6 levels. Discussion and conclusion: This study highlights antioxidant, anti-inflammatory and anti-septic activities of IRG active fractions, eventually suggesting their usefulness in preventing oxidative damage and inflammatory disorders.
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spelling pubmed-61306962018-09-27 Antioxidant, anti-inflammatory and anti-septic potential of phenolic acids and flavonoid fractions isolated from Lolium multiflorum Choi, Ki-Choon Son, Young-Ok Hwang, Jung-Min Kim, Beom-Tae Chae, Minseon Lee, Jeong-Chae Pharm Biol Research Article Context: Interest has recently renewed in using Lolium multiflorum Lam. (Poaceae) (called Italian ryegrass; IRG) silage as an antioxidant and anti-inflammatory diet. Objective: This study investigated the antioxidant, anti-inflammatory and anti-septic potential of IRG silage and identified the primary components in IRG active fractions. Materials and methods: Total 16 fractions were separated from the chloroform-soluble extract of IRG aerial part using Sephadex LH-20 column before HPLC analysis. Antioxidant and anti-inflammatory activities of the fractions at doses of 0–100 μg/mL were investigated using various cell-free and cell-mediated assay systems. To explore anti-septic effect of IRG fractions, female ICR and BALB/c mice orally received 40 mg/kg of phenolic acid and flavonoid-rich active fractions F(7) and F(8) every other day for 10 days, respectively, followed by LPS challenge. Results: The active fractions showed greater antioxidant and anti-inflammatory potential compared with other fractions. IC(50) values of F(7) and F(8) to reduce LPS-stimulated NO and TNF-α production were around 15 and 30 μg/mL, respectively. Comparison of retention times with authentic compounds through HPLC analysis revealed the presence of caffeic acid, ferulic acid, myricetin and kaempferol in the fractions as primary components. These fractions inhibited LPS-stimulated MAPK and NF-κB activation. Supplementation with F(7) or F(8) improved the survival rates of mice to 70 and 60%, respectively, in LPS-injected mice and reduced near completely serum TNF-α and IL-6 levels. Discussion and conclusion: This study highlights antioxidant, anti-inflammatory and anti-septic activities of IRG active fractions, eventually suggesting their usefulness in preventing oxidative damage and inflammatory disorders. Taylor & Francis 2016-12-09 /pmc/articles/PMC6130696/ /pubmed/27937124 http://dx.doi.org/10.1080/13880209.2016.1266673 Text en © 2016 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group. http://creativecommons.org/licenses/by/4.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Choi, Ki-Choon
Son, Young-Ok
Hwang, Jung-Min
Kim, Beom-Tae
Chae, Minseon
Lee, Jeong-Chae
Antioxidant, anti-inflammatory and anti-septic potential of phenolic acids and flavonoid fractions isolated from Lolium multiflorum
title Antioxidant, anti-inflammatory and anti-septic potential of phenolic acids and flavonoid fractions isolated from Lolium multiflorum
title_full Antioxidant, anti-inflammatory and anti-septic potential of phenolic acids and flavonoid fractions isolated from Lolium multiflorum
title_fullStr Antioxidant, anti-inflammatory and anti-septic potential of phenolic acids and flavonoid fractions isolated from Lolium multiflorum
title_full_unstemmed Antioxidant, anti-inflammatory and anti-septic potential of phenolic acids and flavonoid fractions isolated from Lolium multiflorum
title_short Antioxidant, anti-inflammatory and anti-septic potential of phenolic acids and flavonoid fractions isolated from Lolium multiflorum
title_sort antioxidant, anti-inflammatory and anti-septic potential of phenolic acids and flavonoid fractions isolated from lolium multiflorum
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6130696/
https://www.ncbi.nlm.nih.gov/pubmed/27937124
http://dx.doi.org/10.1080/13880209.2016.1266673
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